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We began analyzing https://link.springer.com/article/10.1007/s00432-017-2361-2, but it redirected us to https://link.springer.com/article/10.1007/s00432-017-2361-2. The analysis below is for the second page.

Title[redir]:
Exosomes-mediated transfer of long noncoding RNA ZFAS1 promotes gastric cancer progression | Journal of Cancer Research and Clinical Oncology
Description:
Background ZFAS1 is a newly identified long noncoding RNA (lncRNA) that promotes tumor growth and metastasis. Exosomes mediate cellular communications in cancer by transmitting active molecules. The presence of ZFAS1 in the circulating exosomes and the roles of exosomal ZFAS1 in gastric cancer (GC) remains unknown. The aim of this study was to investigate the potential roles of exosomal ZFAS1 in GC. Methods The expression of ZFAS1 was examined in the tumor tissues, serum samples, serum exosomes of GC patients and cell lines using qRT-PCR. The correlation between ZFAS1 expression and the clinicopathological characteristics was analyzed. The characteristics of exosomes were identified using transmission electron microscope (TEM), Nanoparticle Tracking Analysis (NTA), and western blot. The biological roles of ZFAS1 in GC cell growth and mobility were investigated using cell counting, cell colony formation, and transwell migration assay. The potential mechanism of ZFAS1 was demonstrated using flow cytometry, western blot, and qRT-PCR. Results ZFAS1 expression was elevated in GC cells, tumor tissues, serum and serum exosomes of GC patients. The increased ZFAS1 expression was significantly correlated with lymphatic metastasis and TNM stage. ZFAS1 knockdown inhibited the proliferation and migration of GC cells by suppressing cell cycle progression, inducing apoptosis, and inhibiting epithelial-mesenchymal transition (EMT). On the contrary, ZFAS1 overexpression promoted the proliferation and migration of GC cells. Moreover, ZFAS1 was present in exosomes and could be transmitted by exosomes to enhance GC cell proliferation and migration. Conclusion ZFAS1 could be delivered by exosomes to promote GC progression, which suggests that ZFAS1 may serve as a potential diagnostic and prognostic biomarker for GC.

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cancer, pubmed, article, google, scholar, zfas, long, noncoding, rna, gastric, cas, exosomes, zhang, expression, central, promotes, cell, jiangsu, clin, research, cells, rnas, potential, biomarker, res, progression, pan, metastasis, proliferation, access, oncol, oncotarget, doioncotarget, dois, zhenjiang, privacy, cookies, content, journal, lncrna, serum, breast, china, tumour, supplementary, material, information, publish, search, lei,

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month download article/chapter long-noncoding rna zfas1 long noncoding rna-loweg lncrna-rmrp promotes carcinogenesis snord-host rna zfas1 zhen-hua huang exosomal lncrna-p21 levels wen-rong xu microrna expression profiles long noncoding rnas peng-cheng jiang lncrna pandar predicts breast cancer cells inhibiting epithelial-mesenchymal transition full article pdf promote cancer progression exosomes-mediated transfer hui qian privacy choices/manage cookies promotes tumor growth clinical oncology aims lncrna pandar regulates coding rna pandar coding rna chromogenic coding rna ai364715 epigenetically repressing klf2 competing endogenous rna promising cancer biomarker promote gc progression coding rnas zfas1 zfas1 overexpression promoted zfas1 lncrnas expressions related subjects technology support program natural science foundation distinguish prostate cancer cell colony formation inhibiting cell invasion circulating long rnas breast tumour pathology individual participants included epithelial-mesenchymal transition epithelial-mesenchymal transition epithelial mesenchymal transition medical science coding rnas mirnas zfas1 knockdown inhibited european economic area transmitting active molecules transmission electron microscope

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      description:ZFAS1 is a newly identified long noncoding RNA (lncRNA) that promotes tumor growth and metastasis. Exosomes mediate cellular communications in cancer by transmitting active molecules. The presence of ZFAS1 in the circulating exosomes and the roles of exosomal ZFAS1 in gastric cancer (GC) remains unknown. The aim of this study was to investigate the potential roles of exosomal ZFAS1 in GC. The expression of ZFAS1 was examined in the tumor tissues, serum samples, serum exosomes of GC patients and cell lines using qRT-PCR. The correlation between ZFAS1 expression and the clinicopathological characteristics was analyzed. The characteristics of exosomes were identified using transmission electron microscope (TEM), Nanoparticle Tracking Analysis (NTA), and western blot. The biological roles of ZFAS1 in GC cell growth and mobility were investigated using cell counting, cell colony formation, and transwell migration assay. The potential mechanism of ZFAS1 was demonstrated using flow cytometry, western blot, and qRT-PCR. ZFAS1 expression was elevated in GC cells, tumor tissues, serum and serum exosomes of GC patients. The increased ZFAS1 expression was significantly correlated with lymphatic metastasis and TNM stage. ZFAS1 knockdown inhibited the proliferation and migration of GC cells by suppressing cell cycle progression, inducing apoptosis, and inhibiting epithelial-mesenchymal transition (EMT). On the contrary, ZFAS1 overexpression promoted the proliferation and migration of GC cells. Moreover, ZFAS1 was present in exosomes and could be transmitted by exosomes to enhance GC cell proliferation and migration. ZFAS1 could be delivered by exosomes to promote GC progression, which suggests that ZFAS1 may serve as a potential diagnostic and prognostic biomarker for GC.
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      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
      name:Department of General Surgery, The Affiliated People’s Hospital of Jiangsu University, Zhenjiang, China
      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
      name:Department of General Surgery, The Affiliated People’s Hospital of Jiangsu University, Zhenjiang, China
      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
      name:Department of General Surgery, The Affiliated People’s Hospital of Jiangsu University, Zhenjiang, China
      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
      name:Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, China
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