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google, scholar, pubmed, yeast, cas, article, pombe, schizosaccharomyces, fission, mitochondrial, mitochondria, protocol, model, belenguer, genome, privacy, cookies, content, information, publish, bonnefoy, molecular, cell, cerevisiae, biol, dna, genet, essential, research, search, chiron, guillou, clarkwalker, studies, saccharomyces, cells, chapter, protein, schäfer, rna, gene, genetics, nuclear, data, log, journal, gaisne, methods, respiratory, genetic,

Topics {✒️}

mitochondrial dynamin-related protein mrna-specific translational activation gif-sur-yvette cedex ludwig-maximilians-universität münchen schizosaccharomyces pombe plasmids privacy choices/manage cookies nadh oxidase activity clark-walker nuclear mutation ptp1-1 fission yeast mitochondria humana press fission yeast sit fission yeast enters fission yeast orthologues fission yeast msp1 conserved protein essential journal finder publish fission yeast proteins 10-min dna preparation nucleo-mitochondrial interactions yeast mitochondrial dna european economic area cytokinetic actomyosin rings elongation factor tu exchange factor conserved targeted insertion mutagenesis dnm1p-dependent manner lc-ms/ms université paul sabatier australian national university australian capital territory mitochondrial dna maintenance conditions privacy policy normal mitochondrial morphology mitochondrial mutator phenotype mitochondrial deletion mutants mediates mitochondrial fusion functional dominant mutation rna-processing emmanuelle guillou cell cycle control studying mitochondria accepting optional cookies model unicellular eukaryote mitochondrial genome structure mauricette gaisne protocol cite protocol chiron schizosaccharomyces pombe main content log

Questions {❓}

• (1999) The best yeast?
• (2000) Where does fission yeast sit on the tree of life?
• (2002) What similarity between human and fission yeast proteins is required for orthology?

Schema {🗺️}

ScholarlyArticle:
      headline:Studying Mitochondria in an Attractive Model: Schizosaccharomyces pombe
      pageEnd:105
      pageStart:91
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      genre:
         Springer Protocols
      isPartOf:
         name:Mitochondria
         isbn:
            978-1-59745-365-3
            978-1-58829-667-2
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         name:Humana Press
         logo:
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      author:
            name:Stéphane Chiron
            affiliation:
                  name:Centre de Génétique Moléculaire
                  address:
                     name:Centre de Génétique Moléculaire, Gif-sur-Yvette Cedex, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mauricette Gaisne
            affiliation:
                  name:Centre de Génétique Moléculaire
                  address:
                     name:Centre de Génétique Moléculaire, Gif-sur-Yvette Cedex, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Emmanuelle Guillou
            affiliation:
                  name:Université Paul Sabatier
                  address:
                     name:Laboratoire Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, Université Paul Sabatier, Toulouse Cedex, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Pascale Belenguer
            affiliation:
                  name:Université Paul Sabatier
                  address:
                     name:Laboratoire Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, Université Paul Sabatier, Toulouse Cedex, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:G. Desmond Clark-Walker
            affiliation:
                  name:Australian National University
                  address:
                     name:Molecular Genetics and Evolution Group, Research School of Biological Sciences, Australian National University, Canberra, Australia
                     type:PostalAddress
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                     name:Centre de Génétique Moléculaire, Gif-sur-Yvette Cedex, France
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      keywords:Cytochrome spectra, fractionation, gene disruption, mitochondria preparation, petite-negative yeast, respiratory mutants, Schizosaccharomyces pombe , ura4
      description:The fission yeast Schizosaccharomyces pombe, widely used for studies of cell cycle control and differentiation, provides an alternative and complementary model to the budding yeast Saccharomyces cerevisiae for studies of nucleo-mitochondrial interactions. There are striking similarities between S. pombe and mammalian cells, in both their respiratory physiology and their mitochondrial genome structure. This technical review briefly lists the general and specific properties that are helpful to know when starting to use fission yeast as a model system for mitochondrial studies. In addition, advice is given for cell growth and genetic techniques, tips for disruption of genes involved in respiration are presented, and a basic differential centrifugation protocol is provided for the isolation of purified mitochondria that are suitable for diverse applications such as subfractionation and in vitro import.
      datePublished:2007
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      name:Humana Press
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      address:
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               type:PostalAddress
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            address:
               name:Laboratoire Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, Université Paul Sabatier, Toulouse Cedex, France
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      name:Centre de Génétique Moléculaire, Gif-sur-Yvette Cedex, France
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