We are analyzing https://link.springer.com/article/10.1007/bf00410438.

Title:
The mitochondrial genome of the fission yeast Schizosaccharomyces pombe | Current Genetics
Description:
The three mutator strains ana r-8, ana r-14, and diu r-301 were shown to produce respiratory deficient mutants at different rates. The frequency of respiratory deficient mutants in a culture could be increased by adding ethidium bromide. According to their cytochrome spectra and enzymatic activities they form three classes, namely mutants defective in cytochrome oxidase, in cytochrome b, and in both cytochromes. By restriction enzyme analysis of mitochondrial DNA from about 100 mutants, 22 deletion mutants were identified. The deletions, ranging from 50 to 1,500 base pairs were physically mapped. Deletions were localized in the genes coding for subunit 1 of cytochrome oxidase with its two introns, within the cytochrome b gene and its intron, and within the genes for subunits 2 and 3 of cytochrome oxidase. In several cases, where the physical mapping yielded ambiguous results, pairwise genetic crosses ruled out an overlap between two neighbouring deletions. Using these mitochondrial deletion mutants as tester strains, it was shown that only tetrad analysis and chemical haploidization, but not mitotic segregation analysis, allows a decision between chromosomal and mitochondrial inheritance of respiratory deficiency in Schizosaccharomyces pombe.
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Keywords {🔍}

google, scholar, wolf, kaudewitz, genet, article, mitochondrial, mutants, lang, pombe, mol, gen, schweyen, eds, walter, gruyter, berlin, york, respiratory, cytochrome, mitochondria, schizosaccharomyces, access, privacy, cookies, content, analysis, information, publish, search, ahne, deficient, oxidase, deletions, curr, bandlow, usa, burger, data, log, journal, research, genetics, genome, yeast, deletion, september, merloslange, mutator, genes,

Topics {✒️}

maria-ward-straße la month download article/chapter saccharomyces cerevisiae selected ana-maria merlos-lange saccharomyces cerevisiae schizosaccharomyces pombe homolog related subjects mitochondrial deletion mutants full article pdf privacy choices/manage cookies genome-wide view respiratory deficient mutants mitochondrial protein synthesis respiration-deficient mutants mitochondrial genome walter de gruyter de jonge jc schizosaccharomyces pombe european economic area adding ethidium bromide small ribosomal rna wild type allele check access instant access conditions privacy policy september 1984 volume 8 mol gen genet restriction enzyme analysis mitotic segregation analysis accepting optional cookies cytoplasmic mutator article ahne main content log mt genes coding journal finder publish mitochondrial dna = respiratory deficient article log 22 deletion mutants mitochondrial inheritance merlos-lange lang bf article cite ana r-14 neighbouring deletions mutants defective privacy policy personal data respiratory deficiency slonimski pp

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         description:The three mutator strains ana r-8, ana r-14, and diu r-301 were shown to produce respiratory deficient mutants at different rates. The frequency of respiratory deficient mutants in a culture could be increased by adding ethidium bromide. According to their cytochrome spectra and enzymatic activities they form three classes, namely mutants defective in cytochrome oxidase, in cytochrome b, and in both cytochromes. By restriction enzyme analysis of mitochondrial DNA from about 100 mutants, 22 deletion mutants were identified. The deletions, ranging from 50 to 1,500 base pairs were physically mapped. Deletions were localized in the genes coding for subunit 1 of cytochrome oxidase with its two introns, within the cytochrome b gene and its intron, and within the genes for subunits 2 and 3 of cytochrome oxidase. In several cases, where the physical mapping yielded ambiguous results, pairwise genetic crosses ruled out an overlap between two neighbouring deletions. Using these mitochondrial deletion mutants as tester strains, it was shown that only tetrad analysis and chemical haploidization, but not mitotic segregation analysis, allows a decision between chromosomal and mitochondrial inheritance of respiratory deficiency in Schizosaccharomyces pombe.
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      headline:The mitochondrial genome of the fission yeast Schizosaccharomyces pombe
      description:The three mutator strains ana r-8, ana r-14, and diu r-301 were shown to produce respiratory deficient mutants at different rates. The frequency of respiratory deficient mutants in a culture could be increased by adding ethidium bromide. According to their cytochrome spectra and enzymatic activities they form three classes, namely mutants defective in cytochrome oxidase, in cytochrome b, and in both cytochromes. By restriction enzyme analysis of mitochondrial DNA from about 100 mutants, 22 deletion mutants were identified. The deletions, ranging from 50 to 1,500 base pairs were physically mapped. Deletions were localized in the genes coding for subunit 1 of cytochrome oxidase with its two introns, within the cytochrome b gene and its intron, and within the genes for subunits 2 and 3 of cytochrome oxidase. In several cases, where the physical mapping yielded ambiguous results, pairwise genetic crosses ruled out an overlap between two neighbouring deletions. Using these mitochondrial deletion mutants as tester strains, it was shown that only tetrad analysis and chemical haploidization, but not mitotic segregation analysis, allows a decision between chromosomal and mitochondrial inheritance of respiratory deficiency in Schizosaccharomyces pombe.
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         Deletions
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         Petite negative yeast
         Microbial Genetics and Genomics
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