
NCBI . NLM . NIH . GOV {
}
Title[redir]:
CRISPR Display: A modular method for locus-specific targeting of long noncoding RNAs and synthetic RNA devices in vivo - PMC
Description:
Noncoding RNAs (ncRNAs) comprise an important class of regulatory molecules that mediate a vast array of biological processes. This broad functional capacity has also facilitated the design of artificial ncRNAs with novel functions. To further ...
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ππ Tremendous Traffic: 10M - 20M visitors per month
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Keywords {π}
doi, fig, rna, supplementary, cells, pubmed, google, scholar, pmc, dcas, article, crispdisp, constructs, activation, free, domains, sgrna, top, expression, assays, cell, loci, reporter, table, methods, bridged, dna, complexes, luciferase, gene, lncrna, plasmid, functional, nature, sequence, experiments, targeted, int, imaging, technologies, rnas, natural, figs, construct, media, performed, pool, accessory, artificial, life,
Topics {βοΈ}
genome-scale crispr-mediated control 100 Β΅g/ml poly-l-lysine l7ae-kink-turn rnp switch nunc lab-tek glass high-efficiency crisp-disp construct high-resolution structure/function analysis split-cas9 enzyme complex genome-scale transcriptional activation induce double-stranded breaks minimal βprnaβ stem-loop34 crisp-disp enables modular noncoding rna revolution-trashing insert restores base-pairing pmc beta search pol iii-driven counterparts19 crispr-cas9-based transcription factors results adapting crispr-cas9 tailed t-test relative rna-guided crispr cas9 pyogenes d10a/h841a cas9 optimized crispr/cas system immunoprecipitated dcas9β’int-n25pool complexes viable crisp-disp substrates viral rna-protein complex single plasmid maxi-prep buffer-equilibrated magnetic protein crispr rna-guided activation programs cas9 dna-targeting rna-guided gene activation spinach2-appended telomere-targeting sgrna rna-catalyzed rna polymerization rna/binding-protein pairs xl10-gold ultracompetent cells engineered crispr-cas9 complex standard restriction enzyme-based bearing stem-loops recognized internal pp7 stem-loops maintaining rna-directed localization test crisp-disp function rna-deploying βconduitsβ targeted specific rna-protein interactions spinach2 ligand dfhbi-1t crispr-based biotechnology applications17β22 orthogonal rna-binding proteins ice-cold annealing buffer rnase-free dnas sequence-specific tal effectors natural lncrnas post-transcriptionally single crisp-disp experiment applications crispr-display enables
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- Learn how profitable https://github.com/ncbi is on a monthly basis
- How much does https://www.google.com/maps/place/8600+Rockville+Pike,+Bethesda,+MD+20894/%4038.9959508, -77.101021,17z/data%3D!3m1!4b1!4m5!3m4!1s0x89b7c95e25765ddb%3A0x19156f88b27635b8!8m2!3d38.9959508! 4d-77.0988323 rake in every month?
- How much profit is https://www.nlm.nih.gov/web_policies.html making per month?
- What is the earnings of https://www.nih.gov/institutes-nih/nih-office-director/office-communications-public-liaison/freedom-information-act-office?
- What's https://www.hhs.gov/vulnerability-disclosure-policy/index.html's gross income?
- What are the earnings of https://support.nlm.nih.gov/?pagename=cloudpmc-viewer%3Apmc%3Aarticle%3Amanuscript?
- How much cash flow does https://www.nlm.nih.gov/accessibility.html have monthly?
- What's the profit of https://www.nlm.nih.gov/careers/careers.html?
- How much income does https://www.nlm.nih.gov/ have?
- What's the total monthly financial gain of https://www.nih.gov/?
- How much does https://www.hhs.gov/ pull in?
- How profitable is https://www.usa.gov/?
Analytics and Tracking {π}
- Google Analytics
- Google Analytics 4
- Google Tag Manager
Libraries {π}
- jQuery
- jQuery module (jquery-3.6.0)
- Lazy.js
- Zoom.js
Emails and Hosting {βοΈ}
Mail Servers:
- nihcesxway.hub.nih.gov
- nihcesxway2.hub.nih.gov
- nihcesxway3.hub.nih.gov
- nihcesxway4.hub.nih.gov
- nihcesxway5.hub.nih.gov
Name Servers:
- dns1-ncbi.ncbi.nlm.nih.gov
- dns2-ncbi.ncbi.nlm.nih.gov
- lhcns1.nlm.nih.gov
- lhcns2.nlm.nih.gov
CDN Services {π¦}
- Ncbi