
DOI . ORG {
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Title[redir]:
TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions | Genome Biology | Full Text
Description:
TopHat is a popular spliced aligner for RNA-sequence (RNA-seq) experiments. In this paper, we describe TopHat2, which incorporates many significant enhancements to TopHat. TopHat2 can align reads of various lengths produced by the latest sequencing technologies, while allowing for variable-length indels with respect to the reference genome. In addition to de novo spliced alignment, TopHat2 can align reads across fusion breaks, which can occur after genomic translocations. TopHat2 combines the ability to identify novel splice sites with direct mapping to known transcripts, producing sensitive and accurate alignments, even for highly repetitive genomes or in the presence of pseudogenes. TopHat2 is available at http://ccb.jhu.edu/software/tophat .
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Keywords {π}
reads, tophat, alignments, genome, alignment, read, rnaseq, mapping, pubmed, figure, table, splice, spliced, genes, article, number, sites, edit, human, file, star, mapsplice, gene, indels, additional, gsnap, realignment, transcripts, annotations, exons, aligned, pseudogene, results, google, scholar, central, accuracy, data, pseudogenes, distance, methods, authors, experiments, full, original, size, rum, cas, multiple, programs,
Topics {βοΈ}
keywords=e-mtab-513&expandefo= springer nature author information authors supplementary material simple command-line switch rna-seq unified mapper rna-seq experimental designs authors scientific editing rna-seq reads gathered tthe rna-seq reads real rna-seq dataset call short-anchored reads rna-seq mapping algorithms fixed-length k-mers enhances indel-finding ability exon-spanning reads privacy choices/manage cookies entire human genome rna-seq alignment algorithms rna-seq mapping tools real rna-seq reads years splice site authorsβ original file full-length transcripts defined short-anchored reads separately bmc rna-seq read alignment full size image rna-seq alignment program junction-spanning reads extend average exon length simulated rna-seq data core read-alignment engine abbreviations bp multiple sequencing runs long paired-end reads rna-sequencing analysis software 1474-760x contact resulting edit distance edit distance values entire read sequence rna-seq dataset article kim 1186/gb-2013-14-4-r36 share spliced-read alignments increases figures s3-s4 deletion rna-seq transcriptome mapping improves rna-seq mapping
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headline:TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions
description:TopHat is a popular spliced aligner for RNA-sequence (RNA-seq) experiments. In this paper, we describe TopHat2, which incorporates many significant enhancements to TopHat. TopHat2 can align reads of various lengths produced by the latest sequencing technologies, while allowing for variable-length indels with respect to the reference genome. In addition to de novo spliced alignment, TopHat2 can align reads across fusion breaks, which can occur after genomic translocations. TopHat2 combines the ability to identify novel splice sites with direct mapping to known transcripts, producing sensitive and accurate alignments, even for highly repetitive genomes or in the presence of pseudogenes. TopHat2 is available at
http://ccb.jhu.edu/software/tophat
.
datePublished:2013-04-25T00:00:00Z
dateModified:2013-04-25T00:00:00Z
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Splice Site
Edit Distance
Entire Human Genome
Multiple Exon
Transcriptome Mapping
Animal Genetics and Genomics
Human Genetics
Plant Genetics and Genomics
Microbial Genetics and Genomics
Bioinformatics
Evolutionary Biology
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headline:TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions
description:TopHat is a popular spliced aligner for RNA-sequence (RNA-seq) experiments. In this paper, we describe TopHat2, which incorporates many significant enhancements to TopHat. TopHat2 can align reads of various lengths produced by the latest sequencing technologies, while allowing for variable-length indels with respect to the reference genome. In addition to de novo spliced alignment, TopHat2 can align reads across fusion breaks, which can occur after genomic translocations. TopHat2 combines the ability to identify novel splice sites with direct mapping to known transcripts, producing sensitive and accurate alignments, even for highly repetitive genomes or in the presence of pseudogenes. TopHat2 is available at
http://ccb.jhu.edu/software/tophat
.
datePublished:2013-04-25T00:00:00Z
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Splice Site
Edit Distance
Entire Human Genome
Multiple Exon
Transcriptome Mapping
Animal Genetics and Genomics
Human Genetics
Plant Genetics and Genomics
Microbial Genetics and Genomics
Bioinformatics
Evolutionary Biology
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