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Title[redir]:
VPS35 in Dopamine Neurons Is Required for Endosome-to-Golgi Retrieval of Lamp2a, a Receptor of Chaperone-Mediated Autophagy That Is Critical for α-Synuclein Degradation and Prevention of Pathogenesis of Parkinson's Disease - PMC
Description:
Vacuolar protein sorting-35 (VPS35) is essential for endosome-to-Golgi retrieval of membrane proteins. Mutations in the VPS35 gene have been identified in patients with autosomal dominant PD. However, it remains poorly understood if and how VPS35 ...
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Keywords {🔍}
vps, neurons, lampa, doi, αsynuclein, mice, fig, pubmed, google, scholar, cells, analysis, lamp, pmc, degradation, article, free, reduced, protein, mutant, mouse, deficiency, vpsdeficient, results, reduction, retrieval, cell, data, brain, sem, presented, monthold, disease, shown, endosometogolgi, snpc, quantification, representative, pathogenesis, str, expression, increase, images, aged, retromer, mutation, accumulation, impaired, levels, western,
Topics {✒️}
paav-cdna-egfp-t2a-v5 vector aav5-vps35-d620n-infected da neurons bis-tris sds-page gel vps35-lamp2a-α-synuclein degradation pathway aav-gfp-vps35-d620n infection cma-mediated α-synuclein degradation aav5-vps35-d620n-infected vm pd-linked vps35-d620n mutation disease-related mutant alpha-synuclein aav-gfp-infected control neurons vps35-d620n-expressing nlt cells ampa-type glutamate receptors mirna-vps35-emgfp expression vector pmc beta search vps35-deficient snpc-da neurons pd-relevant neuropathological feature grasp-marked trans-golgi network boosting chaperone-mediated autophagy exogenous gfp-fused vps35-wt ci-m6pr-regulated lysosome function s655 phosphorylation state-dependent gfp-infected cells expressing eea1-labeled early endosomes netrin-1-induced plc signalling retromer-mediated direct sorting model-guided microarray implicates wild type alpha-synuclein pd-linked vps35 mutant ice-cold perchloric acid chaperone-mediated autophagy markers mouse anti p62/sqstm1 d620n-expressing da neurons real-time pcr analysis mediate α-synuclein degradation α-synuclein puncta size α-synuclein increase selectively pmc copyright notice pd-linked vps35 mutation intracellular α-synuclein signals vps35-d620n-expressing neurons vps35-deficient da neurons phosphatidylinositol transfer protein-alpha alpha-synuclein impairs macroautophagy digital grip-strength meter promoting α-synuclein degradation β-gal activity reached vps35-deficient nlt cells tubular-based endosomal sorting α-synuclein exhibits monomeric vps35-d620n-expressing cells
Questions {❓}
- How does VPS35 deficiency result in α-synuclein-accumulation?
- How does VPS35 regulate CMA and lysosome function?
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