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We began analyzing https://journals.biologists.com/jcs/article/123/5/756/31477/Retromer-mediated-direct-sorting-is-required-for, but it redirected us to https://journals.biologists.com/jcs/article/123/5/756/31477/Retromer-mediated-direct-sorting-is-required-for. The analysis below is for the second page.

Title[redir]:
Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron transporter DMT1 | Journal of Cell Science | The Company of Biologists
Description:
Endosomal recycling of the mammalian iron transporter DMT1 is assumed to be important for efficient and rapid uptake of iron across the endosomal membrane in the transferrin cycle. Here, we show that the retromer, a complex that mediates retrograde transport of transmembrane cargoes from endosomes to the trans-Golgi network, is required for endosomal recycling of DMT1-II, an alternative splicing isoform of DMT1. Bacterially expressed Vps26-Vsp29-Vsp35 trimer, a retromer cargo recognition complex, specifically binds to the cytoplasmic tail domain of DMT1-II in vitro. In particular, this binding is dependent on a specific hydrophobic motif of DMT1-II, which is required for its endosomal recycling. DMT1-II colocalizes with the Vps35 subunit of the retromer in TfR-positive endosomes. Depletion of the retromer by siRNA against Vps35 leads to mis-sorting of DMT1-II to LAMP2-positive structures, and expression of siRNA-resistant Vps35 can rescue this effect. These findings demonstrate that the retromer recognizes the recycling signal of DMT1-II and ensures its proper endosomal recycling.

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Keywords {🔍}

dmtii, recycling, retromer, cells, vps, endosomal, fig, google, scholarcrossref, cell, tail, sorting, iron, endosomes, proteins, protein, complex, membrane, domain, cytoplasmic, localization, dmt, motif, structures, analysis, igg, amino, pclamp, signal, colocalization, lamppositive, expression, mutant, sirna, seaman, lamp, alexa, fluor, conjugated, acid, sequence, hep, hours, biol, cimpr, vpsdepleted, transporter, tfr, required, mutants,

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horseradish-peroxidase-conjugated anti-goat igg open pdf horseradish-peroxidase-conjugated anti-mouse pires neo-gfp-sirnar-vps35wt plasmid gst-gfp fusion-protein-immobilized column isopropyl-1-thio-β-d-galactopyranoside pgex-5x-1-ci-mpr tail plasmid gst-dmt1-ii tail wild-type goat anti-mouse igg research project grants goat anti-rabbit igg goat anti-rat igg sirna-resistant vps35 wild-type article information cation-independent mannose-6-phosphate receptor open menu gfp-dmt1-ii wild-type pires neo-gfp plasmid vps35 double-positive punctate-structures goat anti-vps35 pab rabbit anti-ha pab rat anti-ha mab rabbit anti-gfp pab pires neo-3×flag-vps26 wild-type gfp-vps35 3×flag-tagged retromer complex flag-tagged retromer complex generate pires neo-gfp gst-dmt1-ii tail y555a s3 s4 manuscripts 3×flag-vps26-vps29-vps35-his6 mouse anti-lamp2 mab anti-rabbit igg 3×ha-tagged dmt1-ii cargo-recognition complex composed stealth™/sirna duplex oligoribonucleotides mouse anti-tfr mab adp-ribosylation factor-binding retromer cargo-recognition complex cargo-selective retromer subcomplex ni-nta agarose column gfp-tagged dmt1-ii dmt1-ii-cytoplasmic tail domain sirna-resistant vps35 rescued double-positive punctate structures sorting-signal-dependent endosomal recycling dmt1-ii wild-type fast-tracked decision making

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ScholarlyArticle:
      context:https://schema.org
      id:https://journals.biologists.com/jcs/article/123/5/756/31477/Retromer-mediated-direct-sorting-is-required-for
      name:Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron transporter DMT1
      datePublished:2010-03-01
      isPartOf:
         id:https://journals.biologists.com/jcs/issue/123/5
         type:PublicationIssue
         issueNumber:5
         datePublished:2010-03-01
         isPartOf:
            id:https://journals.biologists.com/jcs
            type:Periodical
            name:Journal of Cell Science
            issn:
               1477-9137
      url:https://dx.doi.org/10.1242/jcs.060574
      keywords:
         Endosome
         Retromer
         Recycling
         DMT1
         Iron metabolism
         Transferrin cycle
      inLanguage:en
      copyrightHolder:
      copyrightYear:2025
      publisher:
      author:
            name:Tabuchi, Mitsuaki
            affiliation:Department of Molecular Genetics, Kawasaki Medical School , 577 Matsushima, Kurashiki, Okayama 701-0192 , Japan
            type:Person
            name:Yanatori, Izumi
            affiliation:Department of Molecular Genetics, Kawasaki Medical School , 577 Matsushima, Kurashiki, Okayama 701-0192 , Japan
            type:Person
            name:Kawai, Yasuhiro
            affiliation:Department of Molecular Genetics, Kawasaki Medical School , 577 Matsushima, Kurashiki, Okayama 701-0192 , Japan
            type:Person
            name:Kishi, Fumio
            affiliation:Department of Molecular Genetics, Kawasaki Medical School , 577 Matsushima, Kurashiki, Okayama 701-0192 , Japan
            type:Person
      description:Endosomal recycling of the mammalian iron transporter DMT1 is assumed to be important for efficient and rapid uptake of iron across the endosomal membrane in the transferrin cycle. Here, we show that the retromer, a complex that mediates retrograde transport of transmembrane cargoes from endosomes to the trans-Golgi network, is required for endosomal recycling of DMT1-II, an alternative splicing isoform of DMT1. Bacterially expressed Vps26-Vsp29-Vsp35 trimer, a retromer cargo recognition complex, specifically binds to the cytoplasmic tail domain of DMT1-II in vitro. In particular, this binding is dependent on a specific hydrophobic motif of DMT1-II, which is required for its endosomal recycling. DMT1-II colocalizes with the Vps35 subunit of the retromer in TfR-positive endosomes. Depletion of the retromer by siRNA against Vps35 leads to mis-sorting of DMT1-II to LAMP2-positive structures, and expression of siRNA-resistant Vps35 can rescue this effect. These findings demonstrate that the retromer recognizes the recycling signal of DMT1-II and ensures its proper endosomal recycling.
      pageStart:756
      pageEnd:766
      siteName:The Company of Biologists
      thumbnailURL:https://cob.silverchair-cdn.com/cob/content_public/journal/jcs/issue/123/5/3/m_joces_123_5_cover.png?Expires=1813922614&Signature=yA~gZuDeNt86ne6LV23qOrrwPaGbn1qgrVMsiVL9NnCXk6xZoQkal2Hk0CnSMOIT1PPpEc0y9E~vlaaA5rNAMSBgGMPlQV58U21K-Xk9WcFV09r8Fx2E3~2eWZAE1z-GCUDMHv0cksQl9~LMGzcgpHMU6XoUfB31-bjabeJ-eVphsX2nhrlplkk6vYYhMMlLkRudU1rgehySOCzGqcQj-DyIw5agwLUWSLIVLJzeM0m-MIziw6w8rWzRQQU~IahquTCllYN030yePortm7I2O10roBaRdgAzC5GSiHaX9PB01YQPIiK0NoFdPVl0Q6gsvfmqbd1lWKWJ-x-7UC1H~Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
      headline:Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron transporter DMT1
      image:https://cob.silverchair-cdn.com/cob/content_public/journal/jcs/issue/123/5/3/m_joces_123_5_cover.png?Expires=1813922614&Signature=yA~gZuDeNt86ne6LV23qOrrwPaGbn1qgrVMsiVL9NnCXk6xZoQkal2Hk0CnSMOIT1PPpEc0y9E~vlaaA5rNAMSBgGMPlQV58U21K-Xk9WcFV09r8Fx2E3~2eWZAE1z-GCUDMHv0cksQl9~LMGzcgpHMU6XoUfB31-bjabeJ-eVphsX2nhrlplkk6vYYhMMlLkRudU1rgehySOCzGqcQj-DyIw5agwLUWSLIVLJzeM0m-MIziw6w8rWzRQQU~IahquTCllYN030yePortm7I2O10roBaRdgAzC5GSiHaX9PB01YQPIiK0NoFdPVl0Q6gsvfmqbd1lWKWJ-x-7UC1H~Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
      image:alt:Issue Cover
PublicationIssue:
      id:https://journals.biologists.com/jcs/issue/123/5
      issueNumber:5
      datePublished:2010-03-01
      isPartOf:
         id:https://journals.biologists.com/jcs
         type:Periodical
         name:Journal of Cell Science
         issn:
            1477-9137
Periodical:
      id:https://journals.biologists.com/jcs
      name:Journal of Cell Science
      issn:
         1477-9137
Person:
      name:Tabuchi, Mitsuaki
      affiliation:Department of Molecular Genetics, Kawasaki Medical School , 577 Matsushima, Kurashiki, Okayama 701-0192 , Japan
      name:Yanatori, Izumi
      affiliation:Department of Molecular Genetics, Kawasaki Medical School , 577 Matsushima, Kurashiki, Okayama 701-0192 , Japan
      name:Kawai, Yasuhiro
      affiliation:Department of Molecular Genetics, Kawasaki Medical School , 577 Matsushima, Kurashiki, Okayama 701-0192 , Japan
      name:Kishi, Fumio
      affiliation:Department of Molecular Genetics, Kawasaki Medical School , 577 Matsushima, Kurashiki, Okayama 701-0192 , Japan

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