
DOI . ORG {
}
Title[redir]:
Conditional Expression of Parkinson's Disease-Related Mutant α-Synuclein in the Midbrain Dopaminergic Neurons Causes Progressive Neurodegeneration and Degradation of Transcription Factor Nuclear Receptor Related 1 | Journal of Neuroscience
Description:
α-Synuclein (α-syn) plays a prominent role in the degeneration of midbrain dopaminergic (mDA) neurons in Parkinson
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Keywords {🔍}
mice, neurons, mda, αsyn, expression, nurr, monthold, fig, scholar, ntg, transgenic, midbrain, protein, staining, human, data, thpositive, loss, striatum, snc, openurlcrossrefpubmedgoogle, cells, presented, overexpression, control, dopamine, sem, alphasynuclein, release, genotype, levels, mouse, blot, analysis, littermate, gene, bar, cell, pitx, significant, western, significantly, full, degradation, compared, disease, parkinsons, mutant, age, scale,
Topics {✒️}
littermate pitx3-ires2-tta/ teto-h2bj-gfp/teto-a53t pitx3-ires2-tta/teto-a53t double-transgenic mice pitx3-ires2-tta/teto-h2bj-gfp parkinson's-disease-related mutant alpha-synuclein pitx3/h2bjgfp/a53t triple-transgenic mice obtain pitx3+/ires2-tta-lnl mice ires2-tta-lnl gene-targeting cassette generate pitx3+/ires2-tta mice pitx3-ires2-tta/teto-a53t pitx3/h2bjgfp control double-transgenic disease-linked ala-53→thr mutation pitx3-ires2-tta heterozygous knock α-syn-positive high-molecular-weight spherical α-syn-positive inclusions pitx3+/ires2-tta-lnl mice teto-h2bj-gfp mice ameliorate α-syn-induced degeneration pd-related mutant α-syn disease-related mutant α-synuclein pitx3-ires2-tta mice provide teto-a53t single-transgenic mice disease-related a53t alpha-synuclein pd-related a53t α-syn crossbreeding pitx3-ires2-tta α-syn-dependent dopaminergic dysfunction serum-free medium supplemented α-syn-positive hmw bands autophagy/lysosome-dependent degradation pathways α-syn-induced preferential degeneration α-syn-mediated preferential degeneration autophagy/lysosome-mediated degradation pathway autophagy/lysosomal-mediated degradation pathway α-syn-transfected hek293 cells a53t α-syn-induced loss human α-syn-specific antibody α-syn-induced nurr1 degradation α-syn-induced substantial reduction α-syn-mediated subcellular abnormalities α-syn-induced cell loss α-syn-induced mda neurodegeneration ires2-tta-lnl cassette er/golgi-involved vesicle trafficking mutated human alpha-synuclein truncated human alpha-synuclein histone-gfp fusion protein human α-syn-specific antiserum prefibrillar alpha-synuclein aggregates pitx3-ires2-tta knock pitx3-tta-independent “leaky” expression teto-a53t single-transgenic
Questions {❓}
- ↵ Chesselet MF (2008) In vivo alpha-synuclein overexpression in rodents: a useful model of Parkinson's disease?
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