
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Pcf11 orchestrates transcription termination pathways in yeast - PMC
Description:
In Saccharomyces cerevisiae, short noncoding RNA (ncRNA) generated by RNA polymerase II (Pol II) are terminated by the NRD complex consisting of Nrd1, Nab3, and Sen1. Grzechnik et al. show that Pcf11, a component of the cleavage and polyadenylation ...
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Keywords {🔍}
pcf, nrd, termination, transcription, fig, pol, doi, pubmed, google, scholar, sen, rna, genes, pmc, article, nrddependent, ctd, free, recruitment, analysis, snorna, ser, supplemental, mol, yeast, terminators, cid, phosphorylation, polymerase, interaction, rnadna, regions, cell, complex, downstream, data, ncrna, wild, type, serp, kim, levels, accumulation, end, nab, mutant, domain, chip, snr, biol,
Topics {✒️}
cleavage-dependent/polyadenylation-independent 3′-processing apparatus rpb1 c-terminal domain pol ii–sen1-r302w interactions c-terminal domain pmc beta search high-throughput genomics group rna14/rna15-interacting domain nrd-independent protein-coding genes promote xrn2-dependent termination lacking ctd-binding capacity thiolutin-dependent transcriptional shutoff xrn1-sensitive unstable transcripts nrd1 ctd-interacting domain chip–chip signals smoothed sen1–pol ii interaction cpac-mediated cleavage domain pcf11–ctd interaction prevents protein-coding gene tsss carboxyl-terminal domain transcriptome-wide binding sites nrd-attenuated protein-coding sen1–pol ii binding nrd-dependent transcriptional termination coimmunoprecipitated pol ii org/cgi/doi/10 genome-wide analysis shows single-gene examples confirmed rna–dna hybrid sequence compromised pcf11–ctd interaction nrd-dependent transcription termination direct sen1–rpb1 interaction binds ser5–ser2 phosphorylated pcf11 cid–ctd interaction affect nrd-dependent termination ctd-interacting domain cpac-mediated transcription termination selected nrd-dependent loci short nrd-dependent rna nrd-dependent termination pathway embo long-term fellowship temperature-sensitive pcf11-13 growth nrd-binding sites [nbss] boxh/aca snorna snr5 sen1-r302w-myc analysis pcf11-13 temperature-sensitive phenotype nrd-independent gene pma1 sen1-dependent transcription termination sen1-r302w-myc detection rt-qpcr analysis showed nrd1-dependent transcription units
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