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Author Summary The termination of transcription requires two major multisubunit complexes in budding yeast. These termination complexes are localized at the 3β² end of genes. Recent studies have found the termination factors occupying the 5β² end of genes as well. In this study, we investigate the physiological role of a termination factor at the 5β² end of a gene. Our results show that the CF1 termination complex affects the recruitment of the transcription enzyme RNAP II onto the promoter for reinitiation of transcription. The complex also affects the directionality of transcription of the promoter-bound polymerase. We also found that the looped gene conformation was disrupted in the absence of a functional termination complex. The overall conclusion of these results is that the terminator-bound factors contact the 5β² end of genes due to gene looping, and affect both the recruitment of the polymerase at the promoter for reinitiation, and directionality of the promoter-initiated transcription. Thus, the role of termination factors is not restricted to the 3β² end of the gene, but they are also involved in promoter-associated transcription.
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Keywords {π}
transcription, complex, rna, article, cfa, view, end, google, scholar, figure, gene, cha, termination, mutant, promoter, clp, factors, analysis, rnap, genes, yeast, polymerase, ino, results, antisense, cells, role, tfiib, chip, initiation, region, cell, subunits, temperature, performed, recruitment, reinitiation, minutes, wild, type, looping, rtpcr, shown, transcripts, decrease, general, strain, tro, mrna, pcr,
Topics {βοΈ}
yeast chromosome biology spt-ada-gcn5-acetyltransferase functions probed yeast systems revealed plos genet 9 branson digital sonifier cookies plos plos reverse transcriptase contributed reagents/materials/analysis tools strand-specific rt-pcr analysis strand-specific rt-pcr signal reaction mix 5Γ reaction buffer performed strand-specific rt-pcr open-access article distributed promoter-initiated anti-sense transcripts reverse-transcribing sense transcripts transcriptional coactivator pc4/sub1 reaction volume promoter-initiated anti-sense transcription strand specific rt-pcr engaged rna polymerases nuclear rna polymerases reaction p1-t1 pcr reflects p1-t1 pcr signals reverse-transcribe sense mrna medicine reverse-transcribed rna samples transcriptional termination signals divergent anti-sense transcription rnap ii-mediated transcription tssβ=βtranscription start site chromatin immunoprecipitation analysis f1-r1 pcr represents c-terminus myc-tagged tfiib x-ray film overnight anti-rpb3 antibodies obtained anti-tbp antibodies obtained original author transcribing polymerases terminator-bound factors contact rt-pcr analysis revealed reverse transcription step f2-r1 pcr signals anti-myc antibodies obtained air-dried rna pellet termination-competent cf1a complex upstream polymerases ntps/rnase inhibitor mix
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