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Keywords {🔍}

pcf, ctd, pcfp, termination, cleavage, rna, transcription, binding, processing, end, google, scholar, cid, rnap, polymerase, polyadenylation, mutations, cell, cells, domain, polya, yeast, proteins, protein, vitro, figure, lane, lanes, shown, results, interaction, activity, proudfoot, premrna, factor, crossref, analysis, mutant, essential, mol, expression, transcriptional, mrna, site, manley, wildtype, cterminal, factors, sequence, genes,

Topics {✒️}

hpaii‐digested pbr322 dna ura3‐marked plasmid pfl38‐pcf11 tnt‐coupled transcription–translation system embo journal online gal4 dna‐binding domain amy hin yan tong genome-wide crispr screens 32p‐labelled cyc1 rna pre‐mrna processing machineries synthetic cyc1 pre‐mrna polyclonal anti‐pcf11p serum pre‐mrna processing reactions potential zinc‐finger motifs carboxy‐terminal domain kinase methods yeast strains embo journal vol google scholar minvielle‐sebastia serine/arginine‐rich proteins cpf subunit yhh1p/cft1p termination‐competent condition prior monitors β‐galactosidase expression gst–ctd fusion construct isogenic wild‐type strain wild‐type cf ia open reading frames references alen protein–protein contacts required ch view gst–ctd fusion protein download pdf serine‐arginine‐rich embo journal rna polymerase ii wild‐type cid sequence authors submit trends biochem sci restricted pcf11‐2p function zinc‐finger motifs mrna processing factors pre‐mrna accumulation specific chromatin structures gal4 activation domain rna transcript interferes full‐length transcript hela nuclear extract integrating mrna processing mrna processing machinery gst–δn419‐h6 constructs downstream termination requires conserved amino acids

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