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Title[redir]:
Nuclear surveillance and degradation of hypomodified initiator tRNAMet in S. cerevisiae - PMC
Description:
The tRNA m1A58 methyltransferase is composed of two subunits encoded by the essential genes TRM6 and TRM61 (formerly GCD10 and GCD14). The trm6-504 mutation results in a defective m1A methyltransferase (Mtase) and a temperature-sensitive growth ...
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Keywords {🔍}
trm, trnaimet, degradation, pubmed, doi, google, scholar, trfp, exosome, pretrnaimet, rna, mutant, yeast, trf, hypomodified, pmc, article, trna, dna, ura, rrp, lacking, polya, mrna, rrpδ, fig, analysis, free, phenotype, growth, cells, polymerase, strains, genes, study, gcn, nuclear, wildtype, function, anderson, levels, level, cerevisiae, required, processing, cell, mature, methods, results, total,
Topics {✒️}
high-copy-number plasmid yeplac195 wild-type pre-snornas u14 5-kb sspi-hindiii fragment 5-kb hindiii-pmei fragment pmc beta search single-copy plasmid failed single-copy shuttle vector [doi] [pubmed] hilleren single-copy genomic library [doi] [pubmed] gehrke [doi] [pubmed] kushner ten-minute dna preparation highly conserved stem-loop [doi] [pubmed] sadoff xbai/sali-digested ycplac33 sali/xmai-digested ycplac33 wild-type pre-snornas aminoacyl-transfer rna populations sali/xbai-digested yiplac211 cold spring harbor introducing clai-digested b145 degrades hypomodified pre-trnaimet higher steady-state levels pre-trnaimet lacking m1a58 ureaand 1× tris-borate-edta polyadenylate pre-trnaimet prior org/cgi/doi/10 pre-trnaimet vary significantly 5-foa-resistant colonies retaining conducted marker-rescue experiments human pm-scl dis3/rrp44 gene subcloned identifying polyadenylated pre-trnaimet hypomodified pre-trnaimet occurs wild-type pre-trnaimet genomic dna contained wild-type trnaimet expression plasmid trf4mp2 bearing single species similar conferring temperature sensitivity control initiator-trna binding smai/hindiii prs306 multicopy-number plasmid sali/xmai yeplac195 aberrant pre-trna degradation genomic dna inserts license information pmcid saci/psti yeplac195 swedish cancer society wild-type copy
Questions {❓}
- How does the loss of Trf4p function lead to an increased level of tRNAiMet lacking m1A58?
- The exosome: A proteasome for RNA?
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