
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Microfluidic, marker-free isolation of circulating tumor cells from blood samples - PMC
Description:
The ability to isolate and analyze rare circulating tumor cells (CTCs) has the potential to further our understanding of cancer metastasis and enhance the care of cancer patients. In this protocol, we describe the procedure for isolating rare CTCs ...
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Keywords {🔍}
ctcichip, cells, doi, blood, google, scholar, pubmed, fig, magnetic, cancer, device, ctcs, cell, buffer, tumor, tubing, manifold, pmc, pdms, syringe, article, system, cat, sample, product, free, step, waste, circulating, steps, microfluidic, supplementary, stage, min, flow, patients, design, critical, inlet, pressure, focusing, magnetophoresis, chip, separation, video, devices, top, bottom, array, channel,
Topics {✒️}
ez-link nhs-peg-biotin kit anti-cd45–coated superparamagnetic beads custom-made ctc-ichip2 manifold custom-made ctc-ichip1 manifold anti-cd45–coated magnetic beads poly-lysine–coated glass slide ctc-antigen–dependent capture methods single-cell rna-profiling methods supplemental 2 nihms627581-supplement-supplemental_2 supplemental 3 nihms627581-supplement-supplemental_3 supplemental 4 nihms627581-supplement-supplemental_4 supplemental 5 nihms627581-supplement-supplemental_5 supplemental 6 nihms627581-supplement-supplemental_data nat protoc abstract org/resources/protocols-procedures larger-band-pass dld stage full-length mrna-seq rbc lysis pre-processing tumor-specific staining19 excels pre-established operational ranges micrometer-sized paramagnetic beads pmc beta search supplementary materials included ctc-ichip2 initially separates modified ctc-ichip architecture58 deflecting bead-labeled cells ctc-ichip successfully brings immunoaffinity-based selection system deformability-based cell classification high-magnetic-field gradient colorectal carcinoma-specific antigen chip free-flow magnetophoresis bulk blood-processing methods microvortex-generating herringbone-chip micro-macro interfacing exploits ctc-ichip2 manifold houses ctc-ichip1 lanes blocked cancer type–specific antigens6 anti-human cd45 antibody ctc-ichip2 ctc-ichip1 free beads remain pbs-rinsed vacutainer tube cross-linking reagent tumor antigen–independent detection circulating cytokeratin-positive cells protocol including references ctc-ichip2 magnetic manifold ctc-ichip2 timing 2–5 epcam-labeled magnetic beads ctc-ichip2 manifold properly
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