DOI . ORG {
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Title[redir]:
Detection of Circulating Tumor Cells in Blood Using an Optimized Density Gradient Centrifugation | SpringerLink
Description:
The aim of the study was to compare the new density gradient centrifugation system OncoQuick with the standard density gradient centrifugation system Ficoll for improved tumor cell enrichment in blood of tumor patients. Evaluation of OncoQuick and Ficoll density...
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Keywords {🔍}
tumor, cells, cancer, blood, detection, density, gradient, centrifugation, cell, oncoquick, ficoll, google, scholar, privacy, cookies, content, information, publish, research, paper, circulating, patients, search, system, preview, access, article, res, cas, data, log, journal, molecular, staging, conference, gertler, results, enrichment, evaluation, mononuclear, immunocytochemical, download, microfluidic, chapter, knebel, doeberitz, pubmed, surgery, klinikum, universität,
Topics {✒️}
label-free single-cell separation tissue-specific gene expression density gradient centrifugation micrometastas-tic cancer cells von knebel doeberitz tumor cell density privacy choices/manage cookies reverse transcription pcr cancer ralf gertler ludwig maximilians universität tumor-free probands mononuclear cells separated download preview pdf rare cell populations immunocytochemistry detection system circulating tumor cells disseminated tumor cells enriched cell fraction oncoquick preparation compared european economic area paper cite journal finder publish micrometastatic carcinoma cells conditions privacy policy mononuclear blood cells breast cancer patients gastrointestinal cancer patients venous blood isolates accepting optional cookies book series complete immunocytochemical evaluation knebel doeberitz molecular staging paper gertler main content log patients undergoing surgery check access ethics access cancer research permissions reprints tumor cells cell fraction similar content tumor patients klinikum großhadern germany friedrich heidelberg chapter log cancer res 57 journal publish
Schema {🗺️}
ScholarlyArticle:
headline:Detection of Circulating Tumor Cells in Blood Using an Optimized Density Gradient Centrifugation
pageEnd:155
pageStart:149
image:https://media.springernature.com/w153/springer-static/cover/book/978-3-642-59349-9.jpg
genre:
Medicine
Medicine (R0)
isPartOf:
name:Molecular Staging of Cancer
isbn:
978-3-642-59349-9
978-3-642-63945-6
type:Book
publisher:
name:Springer Berlin Heidelberg
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Ralf Gertler
affiliation:
name:Klinikum rechts der Isar der Technischen Universität München
address:
name:Chirurgische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany
type:PostalAddress
type:Organization
type:Person
name:Robert Rosenberg
affiliation:
type:Person
name:Katrin Fuehrer
affiliation:
type:Person
name:Michael Dahm
affiliation:
type:Person
name:Hjalmar Nekarda
affiliation:
type:Person
name:Joerg Ruediger Siewert
affiliation:
type:Person
keywords:Circulate Tumor Cell, Minimal Residual Disease, Density Gradient Centrifugation, Disseminate Tumor Cell, Tumor Cell Density
description:The aim of the study was to compare the new density gradient centrifugation system OncoQuick with the standard density gradient centrifugation system Ficoll for improved tumor cell enrichment in blood of tumor patients. Evaluation of OncoQuick and Ficoll density gradient centrifugation was performed by flow-cytometry and immunocytochemistry using 10 ml unspiked and tumor cell-spiked blood samples of tumor-free probands. From 10 ml blood, OncoQuick density gradient centrifugation separated a cell fraction which consisted of a mean cell number of 9.5×l04 mononuclear cells compared to 1.8×l07 cells by Ficoll. Density gradient centrifugation of tumor cell-spiked blood samples with OncoQuick and Ficoll led to similar tumor cell recovery rates, between 70% and 90% for both methods. The improved depletion of mononuclear blood cells by OncoQuick simplified further immunocytochemical evaluation of the enriched cell fraction, which could be spun onto 1–2 glass slides by cytocentrifugation. In comparison, the mononuclear cells separated by Ficoll had to be spun onto more than 50 glass slides for complete immunocytochemical evaluation. Consequently, tumor cell density on each cytospin was higher after OncoQuick preparation compared to Ficoll. Density gradient centrifugation with OncoQuick results in higher relative tumor cell enrichment than Ficoll density gradient centrifugation. This simplifies further immunocytochemical tumor cell detection and is a promising tool for the detection of circulating tumor cells in blood of tumor patients.
datePublished:2003
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Book:
name:Molecular Staging of Cancer
isbn:
978-3-642-59349-9
978-3-642-63945-6
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name:Springer Berlin Heidelberg
logo:
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name:Klinikum rechts der Isar der Technischen Universität München
address:
name:Chirurgische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany
type:PostalAddress
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Person:
name:Ralf Gertler
affiliation:
name:Klinikum rechts der Isar der Technischen Universität München
address:
name:Chirurgische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany
type:PostalAddress
type:Organization
name:Robert Rosenberg
affiliation:
name:Katrin Fuehrer
affiliation:
name:Michael Dahm
affiliation:
name:Hjalmar Nekarda
affiliation:
name:Joerg Ruediger Siewert
affiliation:
PostalAddress:
name:Chirurgische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München, Munich, Germany
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