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Long non-coding RNA tagging and expression manipulation via CRISPR/Cas9-mediated targeted insertion | Protein & Cell
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Keywords {π}
targeted, insertion, expression, lncrna, cells, lncrnas, pubmed, established, fig, article, transcriptional, cas, tagging, termination, site, analysis, google, scholar, rna, system, sites, vector, ptenp, gene, genes, pcr, central, inside, noncoding, manipulation, trap, protein, cell, long, qiu, modified, level, examined, cre, crisprcasmediated, functions, chang, crisprcas, neighboring, cheng, zilong, rnas, human, ctrl, dna,
Topics {βοΈ}
explore lncrnas-dna/rna/protein interactions genome-targeting cas9-sgrna complex vector-targeting cas9-sgrna complex crispri-based genome-scale identification crispr/cas9-mediated targeted insertion cell type-specific transcriptomes full size image article download pdf genome-scale deletion screening donor plasmid-targeting sgrna tian-lin cheng conserved multi-exonic structure ms2-egfp fusion protein crispr/cas systems protein-coding messenger rnas transcriptional termination site zilong qiu declares genome-targeting sgrna cmv-puromycin selection cassette lncrna-regulated gene targets lncrna transcriptional termination transcriptional termination sites transcriptional start site crispr-mediated tagging vivo genome editing real-time quantitative pcr combined crispr/cas9 article cheng full access sgrnas targeting sites crispr/cas9 system privacy choices/manage cookies transcriptional start sites cre-mediated recombination targeted insertion site multiplex genome engineering established target insertion entire qiu lab protein-coding ability gene-trap mutagenesis state key laboratory cre-mediated reversion protein-coding rnas protein-coding genes zilong qiu universal donor vector rna transcripts longer gene trap system multi-exonic structures desired genome locus
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