
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR library - PMC
Description:
CRISPR/Cas9 screens have been widely adopted to analyse coding gene functions, but high throughput screening of non-coding elements using this method is more challenging, because indels caused by a single cut in non-coding regions are unlikely to ...
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Keywords {🔍}
doi, lncrnas, pgrnas, cells, fig, cell, supplementary, pubmed, google, scholar, genes, targeting, selected, pmc, cancer, gene, article, library, lncrna, free, pgrna, genomic, screening, negatively, grnas, deletion, expression, control, human, genome, analysis, crisprcas, regions, proliferation, positive, functional, dna, positively, data, negative, lentiviral, promoter, linc, deletions, pairs, designed, sequences, table, infection, essential,
Topics {✒️}
bsmbi-digested scaffold-linker-u6 fragment grna-mediated double-strand break genome-scale crispr-mediated control lentiviral paired-guide rna crispr/cas9-mediated genetic screening crispr/cas9-mediated genomic deletion pmc beta search rna-guided cas9 nucleases oligo-synthesis error rates engineered crispr-cas9 complex dana-farber cancer institute lentivirally-delivered pgrnas affected del velasco-herrera mc produced large-fragment deletions scaffold-linker-u6 fragment crispr/cas9 screen strategy long noncoding rnas lentiviral pgrna-expressing vector genome-scale transcriptional activation trans1-t1 competent cells large-fragment deletions induced rna-seq expression profiles produce large-fragment deletions α-robust rank aggregation pgrna-mediated genomic deletion single guide rnas investigate cis-element function9 krab-dcas9-p2a-mcherry crispr/cas9 pgrna library pgrnas-mediated genomic deletion high-throughput sequencing analysis individual crispr/cas9 knockout ezh2-mediated concordant repression high-throughput sequencing data neighbouring protein-coding genes high-throughput functional genomics grna-coding regions integrated create 60-bp homologies gene-set enrichment analysis large-fragment deletions comprehensive genomic characterization culturing time post-transduction integrative genomic profiling stably expresses cas9 sgrnas carrying egfp overlapping dna fragments crispr-cas9 system crispr/cas9 system robust rank aggregation license information pmcid
Questions {❓}
- Long intronic noncoding RNA transcription: expression noise or expression choice?
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Analytics and Tracking {📊}
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