We are analyzing https://link.springer.com/article/10.1007/s00726-017-2436-z.

Title:
GPNA inhibits the sodium-independent transport system l for neutral amino acids | Amino Acids
Description:
l-γ-Glutamyl-p-nitroanilide (GPNA) is widely used to inhibit the glutamine transporter ASCT2, although it is known that it also inhibits other sodium-dependent amino acid transporters. In a panel of human cancer cell lines, which express the system l transporters LAT1 and LAT2, GPNA inhibits the sodium-independent influx of leucine and glutamine. The kinetics of the effect suggests that GPNA is a low affinity, competitive inhibitor of system l transporters. In Hs683 human oligodendroglioma cells, the incubation in the presence of GPNA, but not ASCT2 silencing, lowers the cell content of leucine. Under the same conditions the activity of mTORC1 is inhibited. Decreased cell content of branched chain amino acids and mTORC1 inhibition are observed in most of the other cell lines upon incubation with GPNA. It is concluded that GPNA hinders the uptake of essential amino acids through system l transporters and lowers their cell content.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 8,149,968 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

The income method remains a mystery to us.

Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Link.springer.com has a secret sauce for making money, but we can't detect it yet.

Keywords {🔍}

pubmed, article, google, scholar, amino, cas, glutamine, cancer, transporter, acid, central, acids, cell, gpna, parma, content, transport, asct, lat, system, transporters, human, cells, access, chiu, mtorc, privacy, essential, cookies, research, leucine, open, growth, author, university, data, information, publish, search, inhibits, neutral, bianchi, ovidio, bussolati, uptake, targeting, ltype, slca, biol, wang,

Topics {✒️}

month download article/chapter sodium-independent transport system γ-glutamyltransferase enzyme activity nγ-aryl glutamine analogues mycn-amplified neuroblastoma cells lat1-4f2hc leu transporter amino acid transporter glutamine transporter asct2 related subjects neutral amino acids full article pdf ovidio bussolati bidirectional transport privacy choices/manage cookies valproic acid induces human oligodendroglioma cells essential amino acids serum/glutamine starvation sodium-independent influx article chiu l-glutamine 3t3-l1 cells check access instant access author correspondence decreased cell content glutamine uptake european economic area preliminary biological evaluation triple-negative basal amino acids 43 amino acids 49 article log tumour growth accepting optional cookies cancer cells reveals leucine induces phosphorylation article cite conditions privacy policy main content log research fellowship journal finder publish promotes mtorc1 activation glutamine depletion asct2 silencing asct2 activation cell lines heavy chain mtorc1 pathway

Questions {❓}

Fuchs BC, Bode BP (2005) Amino acid transporters ASCT2 and LAT1 in cancer: partners in crime?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:GPNA inhibits the sodium-independent transport system l for neutral amino acids
         description: l-γ-Glutamyl-p-nitroanilide (GPNA) is widely used to inhibit the glutamine transporter ASCT2, although it is known that it also inhibits other sodium-dependent amino acid transporters. In a panel of human cancer cell lines, which express the system l transporters LAT1 and LAT2, GPNA inhibits the sodium-independent influx of leucine and glutamine. The kinetics of the effect suggests that GPNA is a low affinity, competitive inhibitor of system l transporters. In Hs683 human oligodendroglioma cells, the incubation in the presence of GPNA, but not ASCT2 silencing, lowers the cell content of leucine. Under the same conditions the activity of mTORC1 is inhibited. Decreased cell content of branched chain amino acids and mTORC1 inhibition are observed in most of the other cell lines upon incubation with GPNA. It is concluded that GPNA hinders the uptake of essential amino acids through system l transporters and lowers their cell content.
         datePublished:2017-05-17T00:00:00Z
         dateModified:2017-05-17T00:00:00Z
         pageStart:1365
         pageEnd:1372
         sameAs:https://doi.org/10.1007/s00726-017-2436-z
         keywords:
            GPNA
            Leucine
            System l
            LAT1
            ASCT2
            Glutamine
            Biochemistry
            general
            Analytical Chemistry
            Biochemical Engineering
            Life Sciences
            Proteomics
            Neurobiology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig3_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig4_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig5_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig6_HTML.gif
         isPartOf:
            name:Amino Acids
            issn:
               1438-2199
               0939-4451
            volumeNumber:49
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Vienna
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Martina Chiu
               affiliation:
                     name:University of Parma
                     address:
                        name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Cosimo Sabino
               affiliation:
                     name:University of Parma
                     address:
                        name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Giuseppe Taurino
               affiliation:
                     name:University of Parma
                     address:
                        name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Massimiliano G. Bianchi
               affiliation:
                     name:University of Parma
                     address:
                        name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Roberta Andreoli
               affiliation:
                     name:University of Parma
                     address:
                        name:Laboratory of Industrial Toxicology, Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Nicola Giuliani
               affiliation:
                     name:University of Parma
                     address:
                        name:Laboratory of Hematology, Department of Medicine and Surgery, University of Parma, University of Parma, 43126 Parma, Italy
                        type:PostalAddress
                     type:Organization
                     name:Azienda Ospedaliero-Universitaria di Parma
                     address:
                        name:Hematology and BMT Center, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Ovidio Bussolati
               url:http://orcid.org/0000-0002-4301-2939
               affiliation:
                     name:University of Parma
                     address:
                        name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:GPNA inhibits the sodium-independent transport system l for neutral amino acids
      description: l-γ-Glutamyl-p-nitroanilide (GPNA) is widely used to inhibit the glutamine transporter ASCT2, although it is known that it also inhibits other sodium-dependent amino acid transporters. In a panel of human cancer cell lines, which express the system l transporters LAT1 and LAT2, GPNA inhibits the sodium-independent influx of leucine and glutamine. The kinetics of the effect suggests that GPNA is a low affinity, competitive inhibitor of system l transporters. In Hs683 human oligodendroglioma cells, the incubation in the presence of GPNA, but not ASCT2 silencing, lowers the cell content of leucine. Under the same conditions the activity of mTORC1 is inhibited. Decreased cell content of branched chain amino acids and mTORC1 inhibition are observed in most of the other cell lines upon incubation with GPNA. It is concluded that GPNA hinders the uptake of essential amino acids through system l transporters and lowers their cell content.
      datePublished:2017-05-17T00:00:00Z
      dateModified:2017-05-17T00:00:00Z
      pageStart:1365
      pageEnd:1372
      sameAs:https://doi.org/10.1007/s00726-017-2436-z
      keywords:
         GPNA
         Leucine
         System l
         LAT1
         ASCT2
         Glutamine
         Biochemistry
         general
         Analytical Chemistry
         Biochemical Engineering
         Life Sciences
         Proteomics
         Neurobiology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig3_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig4_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig5_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00726-017-2436-z/MediaObjects/726_2017_2436_Fig6_HTML.gif
      isPartOf:
         name:Amino Acids
         issn:
            1438-2199
            0939-4451
         volumeNumber:49
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Vienna
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Martina Chiu
            affiliation:
                  name:University of Parma
                  address:
                     name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Cosimo Sabino
            affiliation:
                  name:University of Parma
                  address:
                     name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Giuseppe Taurino
            affiliation:
                  name:University of Parma
                  address:
                     name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Massimiliano G. Bianchi
            affiliation:
                  name:University of Parma
                  address:
                     name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Roberta Andreoli
            affiliation:
                  name:University of Parma
                  address:
                     name:Laboratory of Industrial Toxicology, Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Nicola Giuliani
            affiliation:
                  name:University of Parma
                  address:
                     name:Laboratory of Hematology, Department of Medicine and Surgery, University of Parma, University of Parma, 43126 Parma, Italy
                     type:PostalAddress
                  type:Organization
                  name:Azienda Ospedaliero-Universitaria di Parma
                  address:
                     name:Hematology and BMT Center, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ovidio Bussolati
            url:http://orcid.org/0000-0002-4301-2939
            affiliation:
                  name:University of Parma
                  address:
                     name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Amino Acids
      issn:
         1438-2199
         0939-4451
      volumeNumber:49
Organization:
      name:Springer Vienna
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Parma
      address:
         name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
         type:PostalAddress
      name:University of Parma
      address:
         name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
         type:PostalAddress
      name:University of Parma
      address:
         name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
         type:PostalAddress
      name:University of Parma
      address:
         name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
         type:PostalAddress
      name:University of Parma
      address:
         name:Laboratory of Industrial Toxicology, Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy
         type:PostalAddress
      name:University of Parma
      address:
         name:Laboratory of Hematology, Department of Medicine and Surgery, University of Parma, University of Parma, 43126 Parma, Italy
         type:PostalAddress
      name:Azienda Ospedaliero-Universitaria di Parma
      address:
         name:Hematology and BMT Center, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy
         type:PostalAddress
      name:University of Parma
      address:
         name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Martina Chiu
      affiliation:
            name:University of Parma
            address:
               name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
               type:PostalAddress
            type:Organization
      name:Cosimo Sabino
      affiliation:
            name:University of Parma
            address:
               name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
               type:PostalAddress
            type:Organization
      name:Giuseppe Taurino
      affiliation:
            name:University of Parma
            address:
               name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
               type:PostalAddress
            type:Organization
      name:Massimiliano G. Bianchi
      affiliation:
            name:University of Parma
            address:
               name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
               type:PostalAddress
            type:Organization
      name:Roberta Andreoli
      affiliation:
            name:University of Parma
            address:
               name:Laboratory of Industrial Toxicology, Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy
               type:PostalAddress
            type:Organization
      name:Nicola Giuliani
      affiliation:
            name:University of Parma
            address:
               name:Laboratory of Hematology, Department of Medicine and Surgery, University of Parma, University of Parma, 43126 Parma, Italy
               type:PostalAddress
            type:Organization
            name:Azienda Ospedaliero-Universitaria di Parma
            address:
               name:Hematology and BMT Center, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy
               type:PostalAddress
            type:Organization
      name:Ovidio Bussolati
      url:http://orcid.org/0000-0002-4301-2939
      affiliation:
            name:University of Parma
            address:
               name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
      name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
      name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
      name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
      name:Laboratory of Industrial Toxicology, Department of Medicine and Surgery, University of Parma, 43126 Parma, Italy
      name:Laboratory of Hematology, Department of Medicine and Surgery, University of Parma, University of Parma, 43126 Parma, Italy
      name:Hematology and BMT Center, Azienda Ospedaliero-Universitaria di Parma, Parma, Italy
      name:Laboratory of General Pathology, Department of Medicine and Surgery, University of Parma, Parma, Italy
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(130)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.4s.

LINK . SPRINGER . COM {}