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Title:
Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes | Scientific Reports
Description:
Recent studies have shown that circular RNAs (circRNAs) are abundant, widely expressed in mammals, and can display cell-type specific expression. However, how production of circRNAs is regulated and their precise biological function remains largely unknown. To study how circRNAs might be regulated during colorectal cancer progression, we used three isogenic colon cancer cell lines that differ only in KRAS mutation status. Cellular RNAs from the parental DLD-1 cells that contain both wild-type and G13D mutant KRAS alleles and isogenically-matched derivative cell lines, DKO-1 (mutant KRAS allele only) and DKs-8 (wild-type KRAS allele only) were analyzed using RNA-Seq. We developed a bioinformatics pipeline to identify and evaluate circRNA candidates from RNA-Seq data. Hundreds of high-quality circRNA candidates were identified in each cell line. Remarkably, circRNAs were significantly down-regulated at a global level in DLD-1 and DKO-1 cells compared to DKs-8 cells, indicating a widespread effect of mutant KRAS on circRNA abundance. This finding was confirmed in two independent colon cancer cell lines HCT116 (KRAS mutant) and HKe3 (KRAS WT). In all three cell lines, circRNAs were also found in secreted extracellular-vesicles, and circRNAs were more abundant in exosomes than cells. Our results suggest that circRNAs may serve as promising cancer biomarkers.
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circrnas, cell, pubmed, article, circrna, kras, doi, google, scholar, exosomes, cas, rnas, cells, rna, mutant, analysis, candidates, expression, lines, circular, cancer, genes, nature, results, linear, dks, reads, fig, central, host, downregulated, read, data, backsplice, genome, mates, table, abundant, human, performed, mapped, regulated, dld, wildtype, levels, compared, shown, dko, exosomal, high,
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nature portfolio rna-editing enzymes adar springer international publishing privacy policy author information authors advertising colorectal-tumor development cancer research 73 cell research nature methods 9 nature biotechnology 32 0/ reprints gov/genomes/refseq/bacteria/assembly_summary selected libraries generated nature 280 nature 495 nature 513 nature gu/ag splice sites epithelial-luminal cell state fas apoptosis-promoting receptor gu/ac splice sites scientific reports 5 wild-type cell line wild-type cell lines wild-type kras allele age-dependent neural accumulation back-splicing detection algorithm high-throughput sequencing data existing tools cell-type specific features sequence alignment/map format wild-type kras cells median ewc/enc ratios rna-binding proteins present back-splice read counts rna-binding protein qki fast gapped-read alignment important post-transcriptional regulator41 back-splicing read counts mrna library preparation high-quality circrna candidates bio-rad cfx384 instrument remaining back-splice reads tools back-splice reads mapping linear rna content permissions downstream rna-seq applications rna library construction
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headline:Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes
description:Recent studies have shown that circular RNAs (circRNAs) are abundant, widely expressed in mammals, and can display cell-type specific expression. However, how production of circRNAs is regulated and their precise biological function remains largely unknown. To study how circRNAs might be regulated during colorectal cancer progression, we used three isogenic colon cancer cell lines that differ only in KRAS mutation status. Cellular RNAs from the parental DLD-1 cells that contain both wild-type and G13D mutant KRAS alleles and isogenically-matched derivative cell lines, DKO-1 (mutant KRAS allele only) and DKs-8 (wild-type KRAS allele only) were analyzed using RNA-Seq. We developed a bioinformatics pipeline to identify and evaluate circRNA candidates from RNA-Seq data. Hundreds of high-quality circRNA candidates were identified in each cell line. Remarkably, circRNAs were significantly down-regulated at a global level in DLD-1 and DKO-1 cells compared to DKs-8 cells, indicating a widespread effect of mutant KRAS on circRNA abundance. This finding was confirmed in two independent colon cancer cell lines HCT116 (KRAS mutant) and HKe3 (KRAS WT). In all three cell lines, circRNAs were also found in secreted extracellular-vesicles, and circRNAs were more abundant in exosomes than cells. Our results suggest that circRNAs may serve as promising cancer biomarkers.
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headline:Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes
description:Recent studies have shown that circular RNAs (circRNAs) are abundant, widely expressed in mammals, and can display cell-type specific expression. However, how production of circRNAs is regulated and their precise biological function remains largely unknown. To study how circRNAs might be regulated during colorectal cancer progression, we used three isogenic colon cancer cell lines that differ only in KRAS mutation status. Cellular RNAs from the parental DLD-1 cells that contain both wild-type and G13D mutant KRAS alleles and isogenically-matched derivative cell lines, DKO-1 (mutant KRAS allele only) and DKs-8 (wild-type KRAS allele only) were analyzed using RNA-Seq. We developed a bioinformatics pipeline to identify and evaluate circRNA candidates from RNA-Seq data. Hundreds of high-quality circRNA candidates were identified in each cell line. Remarkably, circRNAs were significantly down-regulated at a global level in DLD-1 and DKO-1 cells compared to DKs-8 cells, indicating a widespread effect of mutant KRAS on circRNA abundance. This finding was confirmed in two independent colon cancer cell lines HCT116 (KRAS mutant) and HKe3 (KRAS WT). In all three cell lines, circRNAs were also found in secreted extracellular-vesicles, and circRNAs were more abundant in exosomes than cells. Our results suggest that circRNAs may serve as promising cancer biomarkers.
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