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We are analyzing https://www.nature.com/articles/srep17948.

Title:
PDGFRα plays a crucial role in connective tissue remodeling | Scientific Reports
Description:
Platelet derived growth factor (PDGF) plays a pivotal role in the remodeling of connective tissues. Emerging data indicate the distinctive role of PDGF receptor-α (PDGFRα) in this process. In the present study, the Pdgfra gene was systemically inactivated in adult mouse (α-KO mouse) and the role of PDGFRα was examined in the subcutaneously implanted sponge matrices. PDGFRα expressed in the fibroblasts of Pdgfra-preserving control mice (Flox mice), was significantly reduced in the sponges in α-KO mice. Neovascularized areas were largely suppressed in the α-KO mice than in the Flox mice, whereas the other parameters related to the blood vessels and endothelial cells were similar. The deposition of collagen and fibronectin and the expression of collagen 1a1 and 3a1 genes were significantly reduced in α-KO mice. There was a significantly decrease in the number and dividing fibroblasts in the α-KO mice and those of macrophages were similar between the two genotypes. Hepatocyte growth factor (Hgf) gene expression was suppressed in Pdgfra-inactivated fibroblasts and connective tissue. The findings implicate the role of PDGFRα-dependent ECM and HGF production in fibroblasts that promotes the remodeling of connective tissue and suggest that PDGFRα may be a relevant target to regulate connective tissue remodeling.
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mice, αko, article, pdgfrα, flox, cells, fig, google, scholar, cas, fibroblasts, tissue, connective, sponge, endothelial, growth, collagen, expression, genotypes, day, blood, hgf, angiogenesis, factor, implanted, fibronectin, cell, significantly, sponges, similar, ecm, supplementary, japan, vascular, nature, role, suppressed, vessels, ctr, analysis, pdgfra, gene, deposition, healing, decreased, implantation, area, areas, wound, ingrowing,

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nature portfolio scientific research privacy policy altered intrinsic nature advertising core research platelet-derived growth factor nature ethanol solutions mouse β-actin platelet-derived growth factors cd31-positive-mimicking tip cells mac-2/ki67-positive proliferating macrophages cellular/extracellular matrix cross-talk alexa-fluor633-conjugated secondary antibodies modified eagle media e-stained cross-sectional area periostin/ki67-positive proliferating fibroblasts 0/ reprints mac-2/phh3-positive dividing macrophages periostin/phh3-positive dividing fibroblasts tamoxifen-induced cre recombinase rabbit polyclonal anti-gfp growth factor-binding sites45 middle rabbit polyclonal anti-periostin pdgfra-preserving pr-mc mice tamoxifen-induced cre activation free access ki67-positive proliferating cells pgk-neo selection cassette real-time pcr analyses rabbit polyclonal anti-vegf pgk-pa signal sequence high germline-differentiating potency rat monoclonal anti-ki67 rabbit monoclonal anti-pdgfrβ rabbit polyclonal anti-pdgfrα rabbit polyclonal anti-pdgfrβ rat monoclonal anti-cd31 increased ki67-positive cells phospho-histone h3 225 mg/kg body weight hgf-administrated α-ko mice cd31-positive endothelial cells social media goat polyclonal anti-pdgfrα pr-mc mice led pgk-neo cassette flanked transgenic mice harboring

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            name:Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
            address:
               name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
               type:PostalAddress
            type:Organization
      name:Hisashi Mori
      affiliation:
            name:Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
            address:
               name:Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
               type:PostalAddress
            type:Organization
      name:Toshihiko Fujimori
      affiliation:
            name:National Institute for Basic Biology
            address:
               name:Division of Embryology, National Institute for Basic Biology, Okazaki, Japan
               type:PostalAddress
            type:Organization
      name:Jie Shen
      affiliation:
            name:Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
            address:
               name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
               type:PostalAddress
            type:Organization
      name:Ran Inoue
      affiliation:
            name:Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
            address:
               name:Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
               type:PostalAddress
            type:Organization
      name:Hirofumi Nishizono
      affiliation:
            name:Life Science Research Center, University of Toyama
            address:
               name:Division of Animal Experimental Laboratory, Life Science Research Center, University of Toyama, Toyama, Japan
               type:PostalAddress
            type:Organization
      name:Hiroshi Itoh
      affiliation:
            name:Graduate School of Medicine, Yamaguchi University
            address:
               name:Department of Molecular Pathology, Graduate School of Medicine, Yamaguchi University, Ube, Japan
               type:PostalAddress
            type:Organization
      name:Masataka Majima
      affiliation:
            name:School of Medicine, Kitasato University
            address:
               name:Department of Pharmacology, School of Medicine, Kitasato University, Kanagawa, Japan
               type:PostalAddress
            type:Organization
      name:David Abraham
      affiliation:
            name:University College London, Royal Free Campus
            address:
               name:Division of Medicine, University College London, Royal Free Campus, London, UK
               type:PostalAddress
            type:Organization
      name:Toshio Miyawaki
      affiliation:
            name:Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
            address:
               name:Department of Pediatrics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
               type:PostalAddress
            type:Organization
      name:Masakiyo Sasahara
      affiliation:
            name:Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
            address:
               name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Department of Pediatrics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Division of Embryology, National Institute for Basic Biology, Okazaki, Japan
      name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Division of Animal Experimental Laboratory, Life Science Research Center, University of Toyama, Toyama, Japan
      name:Department of Molecular Pathology, Graduate School of Medicine, Yamaguchi University, Ube, Japan
      name:Department of Pharmacology, School of Medicine, Kitasato University, Kanagawa, Japan
      name:Division of Medicine, University College London, Royal Free Campus, London, UK
      name:Department of Pediatrics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan
      name:Department of Pathology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan

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