We are analyzing https://link.springer.com/article/10.1007/s10735-007-9124-3.

Title:
In vitro wounding: effects of hypoxia and transforming growth factor β1 on proliferation, migration and myofibroblastic differentiation in an endothelial cell-fibroblast co-culture model | Journal of Molecular Histology
Description:
The adequate reconstitution of human soft tissue wounds requires the coordinated interaction of endothelial cells and fibroblasts during the proliferation phase of healing. Endothelial cells assure neoangiogenesis, fibroblasts fill the defect and provide extracellular matrix proteins, and myofibroblasts are believed to support the reconstitution of microvessels. In the present study, we combined in vitro-wound size measurement and multicolour immunocytochemical staining of co-cultured human dermal microvascular endothelial cells and normal human dermal fibroblasts, recently introduced as co-culture scratch-wound migration assay. Applying antibodies for α-smooth-muscle actin, von Willebrand factor, extra domain A fibronectin and endothelin-1, we were able to monitor proliferation, migration and the differentiation process from fibroblasts to myofibroblasts as a response to hypoxia. Furthermore, we verified, whether transforming growth factor β1 (TGFβ1) and endothelin-1 are able to mediate this response. We show, that proliferation and migration of endothelial cells and fibroblasts decreased under hypoxia. The additional administration of TGFβ1 did not significantly attenuate this decrease. Solely the myofibroblast population in co-culture adapted well to hypoxia, when cultures were supplemented with TGFβ1. Considerating the data concerning TGFβ1 and endothelin-1, we propose a model explaining the cellular interaction during early and late proliferation phase of human wound healing.
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Keywords {🔍}

google, scholar, article, pubmed, cas, cell, human, fibroblasts, wound, growth, endothelial, cells, healing, endothelin, hypoxia, migration, transforming, vitro, factor, myofibroblasts, dermal, proliferation, myofibroblast, gabbiani, differentiation, coculture, microvascular, tgfβ, biol, journal, oberringer, muscle, privacy, cookies, content, extracellular, actin, response, access, chen, invest, pathol, data, publish, search, effects, myofibroblastic, model, meins, bubel,

Topics {✒️}

rac/phosphoinositide 3-kinase/akt-dependent pathway transforming growth factor-beta transforming growth factor-β1 α-smooth-muscle actin α-smooth muscle actin transforming growth factor-beta1 month download article/chapter monika bubel & tim pohlemann growth factor latency von willebrand factor vitro-wound size measurement microvascular endothelial cells skin cell motility anti-angiogenic factors human endothelial cells smooth muscle differentiation endothelin-1 gene expression full article pdf tgfß1 receptor expression wound healing access privacy choices/manage cookies endothelial cell-fibroblast bochaton-piallat ml human dermal fibroblasts paracrine cells important human wound healing hypoxic environments stimulates bronchial fibroblast isolated endothelin-1 induces proliferation cultured human endothelium receptor-dependent activation cell cycle responses human lung fibroblasts human cardiac fibroblasts related subjects bovine aortic endothelial growing cultured fibroblasts cutaneous wound healing wound healing protagonists article oberringer connective tissue remodelling european economic area multicolour immunocytochemical staining 6-diamidino-2-phenylindole ecm `connor-mccourt md plasminogen activator inhibitor du bois rm enhanced contractile phenotype conditions privacy policy molecular histology aims

Questions {❓}

In vitro cultured fetal fibroblasts have myofibroblast-associated characteristics and produce a fibrotic-like environment upon stimulation with TGF-β1: Is there a thin line between fetal scarless healing and fibrosis?
Thiemermann C, Corder R (1992) Is endothelin-1 the regulator of myofibroblast contraction during wound healing?

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         description:The adequate reconstitution of human soft tissue wounds requires the coordinated interaction of endothelial cells and fibroblasts during the proliferation phase of healing. Endothelial cells assure neoangiogenesis, fibroblasts fill the defect and provide extracellular matrix proteins, and myofibroblasts are believed to support the reconstitution of microvessels. In the present study, we combined in vitro-wound size measurement and multicolour immunocytochemical staining of co-cultured human dermal microvascular endothelial cells and normal human dermal fibroblasts, recently introduced as co-culture scratch-wound migration assay. Applying antibodies for α-smooth-muscle actin, von Willebrand factor, extra domain A fibronectin and endothelin-1, we were able to monitor proliferation, migration and the differentiation process from fibroblasts to myofibroblasts as a response to hypoxia. Furthermore, we verified, whether transforming growth factor β1 (TGFβ1) and endothelin-1 are able to mediate this response. We show, that proliferation and migration of endothelial cells and fibroblasts decreased under hypoxia. The additional administration of TGFβ1 did not significantly attenuate this decrease. Solely the myofibroblast population in co-culture adapted well to hypoxia, when cultures were supplemented with TGFβ1. Considerating the data concerning TGFβ1 and endothelin-1, we propose a model explaining the cellular interaction during early and late proliferation phase of human wound healing.
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      description:The adequate reconstitution of human soft tissue wounds requires the coordinated interaction of endothelial cells and fibroblasts during the proliferation phase of healing. Endothelial cells assure neoangiogenesis, fibroblasts fill the defect and provide extracellular matrix proteins, and myofibroblasts are believed to support the reconstitution of microvessels. In the present study, we combined in vitro-wound size measurement and multicolour immunocytochemical staining of co-cultured human dermal microvascular endothelial cells and normal human dermal fibroblasts, recently introduced as co-culture scratch-wound migration assay. Applying antibodies for α-smooth-muscle actin, von Willebrand factor, extra domain A fibronectin and endothelin-1, we were able to monitor proliferation, migration and the differentiation process from fibroblasts to myofibroblasts as a response to hypoxia. Furthermore, we verified, whether transforming growth factor β1 (TGFβ1) and endothelin-1 are able to mediate this response. We show, that proliferation and migration of endothelial cells and fibroblasts decreased under hypoxia. The additional administration of TGFβ1 did not significantly attenuate this decrease. Solely the myofibroblast population in co-culture adapted well to hypoxia, when cultures were supplemented with TGFβ1. Considerating the data concerning TGFβ1 and endothelin-1, we propose a model explaining the cellular interaction during early and late proliferation phase of human wound healing.
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