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Title:
Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion and tumorigenesis in bladder carcinoma cells | Scientific Reports
Description:
Growth differentiation factor-15 (GDF15), a member of the TGF-β superfamily, affects tumor biology of certain cancers, but remains poorly understood in bladder cancer cells. This study determined the expression, regulation, function and potential downstream target genes of GDF15 in bladder carcinoma cells. The transitional papilloma carcionoma cells (RT4) expressed higher levels of GDF15 as compared with the bladder carcinoma cells (HT1376 and T24). Treatments of recombinant human GDF15 (rhGDF15) reduced the proliferations of HT1376 and T24 cells. Expression of GDF15 was upregulated via DNA demethylation and p53. The cell proliferation, invasion and tumorigenesis were reduced in ectopic overexpression of GDF15, while enhanced in GDF15 knockdown. The expressions of mammary serine protease inhibitor (MASPIN) and N-myc downstream-regulated family genes (NDRG1, NDRG2 and NDRG3) were upregulated by GDF15 overexpressions and rhGDF15 treatments in bladder carcinoma cells. GDF15 knockdown induced epithelial-mesenchymal transition (EMT) and F-actin polarization in HT1376 cells. Our results suggest that enhanced expressions of MASPIN and N-myc downstream-regulated family genes and the modulation of EMT may account for the inhibitory functions of GDF15 in the cell proliferation, invasion and tumorigenesis of bladder carcinoma cells. The GDF15 should be considered as a tumor suppressor in human bladder carcinoma cells.
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nature portfolio privacy policy epithelial-mesenchymal transition markers research center nature n-myc downstream-regulated gene advertising epithelial-mesenchymal transition 0/ reprints japan anti-pd-l1 resistance transitional cell tumour tzu-yi lee molecular regulatory mechanisms yu-hsiang lin real-time pcr system combined mts/phenazine methosulfate texas red x-phalloidin macrophage inhibitory cytokine-1 10 μg/ml puromycin dihydrochloride target gene/β-actin 3h-thymidine incorporation assay tumour‐specific antigen p53 wild-type muscle-invasive bladder cancer ht-colsi cell group 3h-thymidine incorporation assays ht-dna cell group development prominent f-actin expression f-actin protein expression growth differentiation factor-15 growth/differentiation factor-15 t24-dna cell group chang gung university permissions urinary bladder carcinoma remains poorly understood downstream target gene nsaid-activated gene-1 cell-type specific tsui-hsia feng atm-dependent p53 expression ht-gdf15si cells decreased n-cadherin expression showed overcome anti-pd-1 blocks cell proliferation tgf-β superfamily inhibits cell proliferation transient p53-overexpressed ht1376
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- Concise review: growth differentiation factor 15 in pathology: a clinical role?
- Growth/differentiation factor-15: prostate cancer suppressor or promoter?
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headline:Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion and tumorigenesis in bladder carcinoma cells
description:Growth differentiation factor-15 (GDF15), a member of the TGF-β superfamily, affects tumor biology of certain cancers, but remains poorly understood in bladder cancer cells. This study determined the expression, regulation, function and potential downstream target genes of GDF15 in bladder carcinoma cells. The transitional papilloma carcionoma cells (RT4) expressed higher levels of GDF15 as compared with the bladder carcinoma cells (HT1376 and T24). Treatments of recombinant human GDF15 (rhGDF15) reduced the proliferations of HT1376 and T24 cells. Expression of GDF15 was upregulated via DNA demethylation and p53. The cell proliferation, invasion and tumorigenesis were reduced in ectopic overexpression of GDF15, while enhanced in GDF15 knockdown. The expressions of mammary serine protease inhibitor (MASPIN) and N-myc downstream-regulated family genes (NDRG1, NDRG2 and NDRG3) were upregulated by GDF15 overexpressions and rhGDF15 treatments in bladder carcinoma cells. GDF15 knockdown induced epithelial-mesenchymal transition (EMT) and F-actin polarization in HT1376 cells. Our results suggest that enhanced expressions of MASPIN and N-myc downstream-regulated family genes and the modulation of EMT may account for the inhibitory functions of GDF15 in the cell proliferation, invasion and tumorigenesis of bladder carcinoma cells. The GDF15 should be considered as a tumor suppressor in human bladder carcinoma cells.
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