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We are analyzing https://www.nature.com/articles/s41467-018-04070-6.

Title:
Autophagy promotes the survival of dormant breast cancer cells and metastatic tumour recurrence | Nature Communications
Description:
Cancer recurrence after initial diagnosis and treatment is a major cause of breast cancer (BC) mortality, which results from the metastatic outbreak of dormant tumour cells. Alterations in the tumour microenvironment can trigger signalling pathways in dormant cells leading to their proliferation. However, processes involved in the initial and the long-term survival of disseminated dormant BC cells remain largely unknown. Here we show that autophagy is a critical mechanism for the survival of disseminated dormant BC cells. Pharmacologic or genetic inhibition of autophagy in dormant BC cells results in significantly decreased cell survival and metastatic burden in mouse and human 3D in vitro and in vivo preclinical models of dormancy. In vivo experiments identify autophagy gene autophagy-related 7 (ATG7) to be essential for autophagy activation. Mechanistically, inhibition of the autophagic flux in dormant BC cells leads to the accumulation of damaged mitochondria and reactive oxygen species (ROS), resulting in cell apoptosis. Highly metastatic dormant cancer cells contribute to breast cancer recurrence, but the underlying mechanism is less understood. Here, the authors show that dormant breast cancer cells depend on autophagy to ensure their long term survival and distant recurrence
Website Age:
30 years and 10 months (reg. 1994-08-11).

Matching Content Categories {๐Ÿ“š}

  • Science
  • Education
  • Health & Fitness

Content Management System {๐Ÿ“}

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Custom-built

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Traffic Estimate {๐Ÿ“ˆ}

What is the average monthly size of nature.com audience?

๐ŸŒ  Phenomenal Traffic: 5M - 10M visitors per month


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How Does Nature.com Make Money? {๐Ÿ’ธ}


Display Ads {๐ŸŽฏ}


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Direct Advertisers (10)
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Reseller Advertisers (38)
conversantmedia.com, rubiconproject.com, pubmatic.com, appnexus.com, openx.com, smartadserver.com, lijit.com, sharethrough.com, video.unrulymedia.com, google.com, yahoo.com, triplelift.com, onetag.com, sonobi.com, contextweb.com, 33across.com, indexexchange.com, media.net, themediagrid.com, adform.com, richaudience.com, sovrn.com, improvedigital.com, freewheel.tv, smaato.com, yieldmo.com, amxrtb.com, adyoulike.com, adpone.com, criteo.com, smilewanted.com, 152media.info, e-planning.net, smartyads.com, loopme.com, opera.com, mediafuse.com, betweendigital.com

How Much Does Nature.com Make? {๐Ÿ’ฐ}


Display Ads {๐ŸŽฏ}

$63,100 per month
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Keywords {๐Ÿ”}

cells, autophagy, bme, dormant, hcq, metastatic, day, fig, cell, pubmed, article, cancer, days, inhibition, google, scholar, culture, treatment, cas, data, tumour, treated, mice, comparisons, mitochondrial, col, sem, burden, supplementary, compared, proliferative, matrices, nature, survival, lung, assay, central, analysis, dormancy, results, vivo, fibrosis, twosided, autophagic, proliferation, significantly, vitro, observed, expression, wells,

Topics {โœ’๏ธ}

/product-category/physiologic-cell-culture/extracellular-matrix-proteins/ nature portfolio privacy policy andalusian regional ministry intramural research program thermo scientific anti-cyclophillin proliferative index determined advertising nature 529 nature tandem reporter mcherry-gfp-lc3 reprints col-i-enriched fibrotic environment thermo fisher scientific research cd1nu/nu athymic mice isolate mcherry-gfp-lc3 d2 cdnu/nu athymic mice article vera-ramirez mannโ€“whitney u-test tandem fluorescent-tagged lc3 library preparation references maishman cd1nu/nu mice injected adenovector-mediated gene transfer mcherry-gfp-lc3 cells showed mcherry-gfp-lc3 cells isolated supplementary figuresย 9 50โ€‰mg/kg body weight mcherry-gfp-lc3 cells grown original author social media complete media methods cell lines cross sections genitourinary medical oncology park2/parkin-dependent mitophagy mda-mb-231 cell lines initial orga-specific metastasis cytotox-glo cytoxocity assay frequent post-translational modifications develop oxidative-based therapies double-positive puncta total tumour burden/lung mda-mb-231 viable cells full size image mcherry-gfp-lc3 cells author lipidated form lc3-ii average tumour burden/lung

Schema {๐Ÿ—บ๏ธ}

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         headline:Autophagy promotes the survival of dormant breast cancer cells and metastatic tumour recurrence
         description:Cancer recurrence after initial diagnosis and treatment is a major cause of breast cancer (BC) mortality, which results from the metastatic outbreak of dormant tumour cells. Alterations in the tumour microenvironment can trigger signalling pathways in dormant cells leading to their proliferation. However, processes involved in the initial and the long-term survival of disseminated dormant BC cells remain largely unknown. Here we show that autophagy is a critical mechanism for the survival of disseminated dormant BC cells. Pharmacologic or genetic inhibition of autophagy in dormant BC cells results in significantly decreased cell survival and metastatic burden in mouse and human 3D in vitro and in vivo preclinical models of dormancy. In vivo experiments identify autophagy gene autophagy-related 7 (ATG7) to be essential for autophagy activation. Mechanistically, inhibition of the autophagic flux in dormant BC cells leads to the accumulation of damaged mitochondria and reactive oxygen species (ROS), resulting in cell apoptosis. Highly metastatic dormant cancer cells contribute to breast cancer recurrence, but the underlying mechanism is less understood. Here, the authors show that dormant breast cancer cells depend on autophagy to ensure their long term survival and distant recurrence
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      headline:Autophagy promotes the survival of dormant breast cancer cells and metastatic tumour recurrence
      description:Cancer recurrence after initial diagnosis and treatment is a major cause of breast cancer (BC) mortality, which results from the metastatic outbreak of dormant tumour cells. Alterations in the tumour microenvironment can trigger signalling pathways in dormant cells leading to their proliferation. However, processes involved in the initial and the long-term survival of disseminated dormant BC cells remain largely unknown. Here we show that autophagy is a critical mechanism for the survival of disseminated dormant BC cells. Pharmacologic or genetic inhibition of autophagy in dormant BC cells results in significantly decreased cell survival and metastatic burden in mouse and human 3D in vitro and in vivo preclinical models of dormancy. In vivo experiments identify autophagy gene autophagy-related 7 (ATG7) to be essential for autophagy activation. Mechanistically, inhibition of the autophagic flux in dormant BC cells leads to the accumulation of damaged mitochondria and reactive oxygen species (ROS), resulting in cell apoptosis. Highly metastatic dormant cancer cells contribute to breast cancer recurrence, but the underlying mechanism is less understood. Here, the authors show that dormant breast cancer cells depend on autophagy to ensure their long term survival and distant recurrence
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      name:Department of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, USA
      name:Laboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, USA
      name:Laboratory of Cancer Biology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, USA

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