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Title:
Half life of yeast messenger RNA | Nature
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WE have determined the half life of Saccharomyces cerevisiae messenger RNA (mRNA) and its associated poly (A) sequences, from the kinetics of their labelling with 14C-adenine. Corrections have been made for changes in the ATP pool specific activity during the labelling period. This method of determining the mRNA half life is preferred to observing the decay of mRNA in the presence of inhibitors because changes in the cell metabolism caused by an inhibitor may also change the mRNA half life1. One of our purposes in determining these half lives is for their use in the characterisation of temperature-sensitive mutants which are defective in macromolecular synthesis2. For this reason we have determined the half lives of mRNA and poly(A) in two different conditions, at 23 and 36 Β°C.
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nature portfolio permissions reprints privacy policy advertising subscribe nature social media nature nature 260 personal data data protection springerlink instant access permissions yeastsaccharomyces cerevisiae leland saccharomyces cerevisiae yeast messenger rna privacy yeast iii steven messenger rna nancy explore content subscription content european economic area temperature-sensitive mutants institutional subscriptions read current transcriptional network general genetics mgg accepting optional cookies journals search log issue learn dna synthesis mutants molecular biology institute manage preferences cell metabolism caused mrna enrichment strategies mrna half life1 content article purchase mrna half life article petersen access los angeles article cite https 1038/260070a0 yeast mutant nierlich rights optional cookies half lives choices conditions rna synthesis
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