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We began analyzing https://link.springer.com/article/10.1007/BF00337788, but it redirected us to https://link.springer.com/article/10.1007/BF00337788. The analysis below is for the second page.

Title[redir]:
The half-life of mRNA in Saccharomyces cerevisiae | Molecular Genetics and Genomics
Description:
The decay kinetics of mRNA was studied in a yeast temperature-sensitive mutant, ts136, which is defective in cytoplasmic RNA production at 37° C. The disappearance of the synthetic capacity of mRNA was determined by withdrawing equal volumes of ts136 cell culture and pulse-labelling with [35S]methionine at various time intervals after the shift to 37° C from 23° C. The synthesized proteins were separated on a two-dimensional gel electrophoretic system and then quantitatively analyzed for their incorporated radioactivities by scintillation counting. Our results show that yeast mRNAs have divergent functional half-lives ranging from 4.5 to 41 min, with an average value of 22 min. Each mRNA exhibits a simple exponential decay with its own characteristic decay pattern. Of the approximately 500 major polypeptides made by yeast cells, which are detectable on autoradiograms of the gels, 80 were arbitrarily selected and the mRNAs coding for those polypeptides were examined for their decay kinetics.

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Keywords {🔍}

google, scholar, saccharomyces, cerevisiae, yeast, bacteriol, article, decay, mrna, mclaughlin, synthesis, rna, privacy, cookies, content, kinetics, access, warner, ribonucleic, acid, information, publish, search, proteins, cells, messenger, biol, data, log, journal, research, molecular, general, halflife, chia, temperaturesensitive, defective, production, cell, chapter, discover, cooper, usa, hartwell, enzyme, nature, download, springer, optional, processing,

Topics {✒️}

month download article/chapter yeast temperature-sensitive mutant rapid uridine pulse-chase cytoplasmic rna production scintillation counting simple exponential decay characteristic decay pattern yeast messenger rna messenger rna synthesis messenger rna turnover saccharomyces cerevisiae published privacy choices/manage cookies article chia related subjects temperature-sensitive mutants full article pdf decay kinetics european economic area withdrawing equal volumes de robichon-szulmajster s-adenosylmethionine pool ribonucleic acid production conditions privacy policy check access messenger ribonucleic acid instant access decay rate yeast mutants defective polypeptide chains saccharomyces cerevisiae eucaryotic ribosomal proteins accepting optional cookies ribonucleic acid synthesis main content log repressed enzyme synthesis journal finder publish metabolism article molecular ts136 cell culture half-life gajewski rights yeast mrna privacy policy article log personal data ribonucleic acid processing books a mrna exhibits general control

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