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LINK . SPRINGER . COM {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. External Links
  11. Analytics And Tracking
  12. Libraries
  13. CDN Services

We are analyzing https://link.springer.com/article/10.1186/s12943-021-01359-x.

Title:
CircPTPRA blocks the recognition of RNA N6-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression | Molecular Cancer
Description:
Background Circular RNAs (circRNAs) have been found to have significant impacts on bladder cancer (BC) progression through various mechanisms. In this study, we aimed to identify novel circRNAs that regulate the function of IGF2BP1, a key m6A reader, and explore the regulatory mechanisms and clinical significances in BC. Methods Firstly, the clinical role of IGF2BP1 in BC was studied. Then, RNA immunoprecipitation sequencing (RIP-seq) analysis was performed to identify the circRNAs interacted with IGF2BP1 in BC cells. The overall biological roles of IGF2BP1 and the candidate circPTPRA were investigated in both BC cell lines and animal xenograft studies. Subsequently, we evaluated the regulation effects of circPTPRA on IGF2BP1 and screened out its target genes through RNA sequencing. Finally, we explored the underlying molecular mechanisms that circPTPRA might act as a blocker in recognition of m6A. Results We demonstrated that IGF2BP1 was predominantly binded with circPTPRA in the cytoplasm in BC cells. Ectopic expression of circPTPRA abolished the promotion of cell proliferation, migration and invasion of BC cells induced by IGF2BP1. Importantly, circPTPRA downregulated IGF2BP1-regulation of MYC and FSCN1 expression via interacting with IGF2BP1. Moreover, the recognition of m6A-modified RNAs mediated by IGF2BP1 was partly disturbed by circPTPRA through its interaction with KH domains of IGF2BP1. Conclusions This study identifies exonic circular circPTPRA as a new tumor suppressor that inhibits cancer progression through endogenous blocking the recognition of IGF2BP1 to m6A-modified RNAs, indicating that circPTPRA may serve as an exploitable therapeutic target for patients with BC.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Education
  • Science
  • Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
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How Does Link.springer.com Make Money? {💸}

We're unsure if the website is profiting.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com could be secretly minting cash, but we can't detect the process.

Keywords {🔍}

igfbp, circptpra, cells, pubmed, article, rna, cell, expression, fig, cancer, google, scholar, myc, cas, overexpression, invasion, fscn, bladder, transfected, mrna, assay, central, migration, analysis, circular, tissues, assays, data, circrnas, control, tumor, protein, zhang, rnas, regulation, images, performed, proliferation, vitro, stably, significantly, progression, study, patients, usa, cycle, results, growth, stability, mrnas,

Topics {✒️}

aff4/nf-κb/myc signaling network hsa-mir-145-zeb1/2-fscn1 pathway tumor volume = π/6 × length×width2 article download pdf mir-182-5p/p27 axis [12] mir-182-5p/p27 axis protein-coding transcripts region protease/phosphatase inhibitor cocktail small interference rnas igf2 mrna-binding proteins imp-1 displays cross-talk flag-tagged full-length cytoplasmic protein–rna complexes cd31-positive intratumoral microvessels positive feed-back regulation m6a-modified rnas mediated c-myc mrna decay rna–protein binding mixture rna n6-methyladenosine methyltransferase mapk signaling transduction kaplan-meier curves indicating increasing rna-sequencing analyses downstream m6a-modified mrnas surprisingly long half-life flag-tagged igf2bp1 protein promotes cap-independent translation fscn1 mrna half-life lncrna lin28b-as1 canonical rna-binding domains distinct rna-binding domains kaplan–meier survival curve 200 μl serum-free medium impairing mirna-directed downregulation underlying molecular mechanisms cy3-labeled circptpra probes invasive bladder cancer noubissi fk igf2bp1-mediated gene regulation important tumor suppressor igf2bp1 single-guide rna igf2bp1-overexpressed xenograft tumors lin28b mrna stability human bladder cancer 6-methyladenosine search search real-time pcr crd-bp mediates stabilization mrna decay assay m6a-dependent binding mode c-myc mrna stability promotes cancer proliferation

Questions {❓}

  • The evolution of bladder cancer genomics: what have we learned and how can we use it?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:CircPTPRA blocks the recognition of RNA N6-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression
         description:Circular RNAs (circRNAs) have been found to have significant impacts on bladder cancer (BC) progression through various mechanisms. In this study, we aimed to identify novel circRNAs that regulate the function of IGF2BP1, a key m6A reader, and explore the regulatory mechanisms and clinical significances in BC. Firstly, the clinical role of IGF2BP1 in BC was studied. Then, RNA immunoprecipitation sequencing (RIP-seq) analysis was performed to identify the circRNAs interacted with IGF2BP1 in BC cells. The overall biological roles of IGF2BP1 and the candidate circPTPRA were investigated in both BC cell lines and animal xenograft studies. Subsequently, we evaluated the regulation effects of circPTPRA on IGF2BP1 and screened out its target genes through RNA sequencing. Finally, we explored the underlying molecular mechanisms that circPTPRA might act as a blocker in recognition of m6A. We demonstrated that IGF2BP1 was predominantly binded with circPTPRA in the cytoplasm in BC cells. Ectopic expression of circPTPRA abolished the promotion of cell proliferation, migration and invasion of BC cells induced by IGF2BP1. Importantly, circPTPRA downregulated IGF2BP1-regulation of MYC and FSCN1 expression via interacting with IGF2BP1. Moreover, the recognition of m6A-modified RNAs mediated by IGF2BP1 was partly disturbed by circPTPRA through its interaction with KH domains of IGF2BP1. This study identifies exonic circular circPTPRA as a new tumor suppressor that inhibits cancer progression through endogenous blocking the recognition of IGF2BP1 to m6A-modified RNAs, indicating that circPTPRA may serve as an exploitable therapeutic target for patients with BC.
         datePublished:2021-04-14T00:00:00Z
         dateModified:2022-11-01T00:00:00Z
         pageStart:1
         pageEnd:17
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s12943-021-01359-x
         keywords:
            Bladder cancer
            circPTPRA
            IGF2BP1
             N -methyladenosine
            Cancer Research
            Oncology
         image:
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            issn:
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               name:Fei Xie
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                     name:Huazhong University of Science and Technology
                     address:
                        name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
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                        name:Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, China
                        type:PostalAddress
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                     name:Huazhong University of Science and Technology
                     address:
                        name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
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               name:Feng Liu
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                     name:Huazhong University of Science and Technology
                     address:
                        name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Hui Zhang
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
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               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jiayin Sun
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Xiaoping Zhang
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                        type:PostalAddress
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               email:[email protected]
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               name:Guosong Jiang
               url:http://orcid.org/0000-0002-7329-3962
               affiliation:
                     name:Huazhong University of Science and Technology
                     address:
                        name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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ScholarlyArticle:
      headline:CircPTPRA blocks the recognition of RNA N6-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression
      description:Circular RNAs (circRNAs) have been found to have significant impacts on bladder cancer (BC) progression through various mechanisms. In this study, we aimed to identify novel circRNAs that regulate the function of IGF2BP1, a key m6A reader, and explore the regulatory mechanisms and clinical significances in BC. Firstly, the clinical role of IGF2BP1 in BC was studied. Then, RNA immunoprecipitation sequencing (RIP-seq) analysis was performed to identify the circRNAs interacted with IGF2BP1 in BC cells. The overall biological roles of IGF2BP1 and the candidate circPTPRA were investigated in both BC cell lines and animal xenograft studies. Subsequently, we evaluated the regulation effects of circPTPRA on IGF2BP1 and screened out its target genes through RNA sequencing. Finally, we explored the underlying molecular mechanisms that circPTPRA might act as a blocker in recognition of m6A. We demonstrated that IGF2BP1 was predominantly binded with circPTPRA in the cytoplasm in BC cells. Ectopic expression of circPTPRA abolished the promotion of cell proliferation, migration and invasion of BC cells induced by IGF2BP1. Importantly, circPTPRA downregulated IGF2BP1-regulation of MYC and FSCN1 expression via interacting with IGF2BP1. Moreover, the recognition of m6A-modified RNAs mediated by IGF2BP1 was partly disturbed by circPTPRA through its interaction with KH domains of IGF2BP1. This study identifies exonic circular circPTPRA as a new tumor suppressor that inhibits cancer progression through endogenous blocking the recognition of IGF2BP1 to m6A-modified RNAs, indicating that circPTPRA may serve as an exploitable therapeutic target for patients with BC.
      datePublished:2021-04-14T00:00:00Z
      dateModified:2022-11-01T00:00:00Z
      pageStart:1
      pageEnd:17
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s12943-021-01359-x
      keywords:
         Bladder cancer
         circPTPRA
         IGF2BP1
          N -methyladenosine
         Cancer Research
         Oncology
      image:
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      isPartOf:
         name:Molecular Cancer
         issn:
            1476-4598
         volumeNumber:20
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Fei Xie
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
                  name: The Affiliated Hospital of Qingdao University
                  address:
                     name:Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Chao Huang
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Feng Liu
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hui Zhang
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xingyuan Xiao
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jiayin Sun
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Xiaoping Zhang
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Guosong Jiang
            url:http://orcid.org/0000-0002-7329-3962
            affiliation:
                  name:Huazhong University of Science and Technology
                  address:
                     name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
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      name:Molecular Cancer
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      address:
         name:Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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         name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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      name:Huazhong University of Science and Technology
      address:
         name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
      address:
         name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
         type:PostalAddress
      name:Huazhong University of Science and Technology
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         type:PostalAddress
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      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Fei Xie
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
            name: The Affiliated Hospital of Qingdao University
            address:
               name:Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, China
               type:PostalAddress
            type:Organization
      name:Chao Huang
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Feng Liu
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Hui Zhang
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Xingyuan Xiao
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Jiayin Sun
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      name:Xiaoping Zhang
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Guosong Jiang
      url:http://orcid.org/0000-0002-7329-3962
      affiliation:
            name:Huazhong University of Science and Technology
            address:
               name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, China
      name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      name:Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

External Links {🔗}(258)

Analytics and Tracking {📊}

  • Google Tag Manager

Libraries {📚}

  • Clipboard.js
  • Prism.js

CDN Services {📦}

  • Crossref

6.11s.