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Keywords {🔍}

igfbp, doi, igfbps, cells, rna, fig, mrna, binding, supplementary, scholar, google, pubmed, pmc, data, proteins, article, myc, free, cell, targets, expression, genes, independent, analysis, protein, mettl, stability, hepg, crd, target, control, human, knockdown, western, hekt, experiments, mrnas, sites, gene, performed, buffer, domains, readers, cancer, translation, calculated, hela, luciferase, wildtype, stress,

Topics {✒️}

nlc-esi-ms/ms analyses targeting fto/m6a/myc/cebpa signaling single-stranded rna-binding proteins17 p-body component usp52/pan2 nonparametric mann–whitney u-test determining read-number cutoffs published par-clip-seq targets22 pcdna3-ha-ha-humancmyc 5x tris-borate-edta buffer [free rna]+[rna-protein] lc-ms/ms fifty contributed reagents/analytic tools regulating pre-mrna splicing pmc beta search optimized crispr/cas system n6-methyladenosine rna demethylase dual-luciferase reporter assay rip-lc-ms/ms metal-ion induced fragmentation 05u/μl dnase rq1 crispr-cas9 mediated knockout n6-methyladenosine-dependent regulation lc-ms/ms quantification overexpressed flag-tagged igf2bps high-confidence rna targets nlc-ms/ms m6a-dependent binding mode transcriptome-wide data analyses crd-bp mediates stabilization mrna half-life analyses nonsense-mediated mrna decay rna-binding protein imp-2 high-throughput sequencing data canonical rna-binding domains post-transcriptional gene regulation published rna-binding protein c-myc mrna stability wilcoxon rank-sum test shared high-confidence targets ythdf2-mediated decay pathway10 tcga pan-cancer studies [google scholar] 48 significant lower guanine-cytosine biotin-labelled ss gel shift assay mrna log2-fold change igf2bp3 high-confidence targets gene-specific m6a assays full length proteins par-clip target genes

Questions {❓}

• Insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs): post-transcriptional drivers of cancer progression?

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