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  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Link.springer.com Make Money
  6. Keywords
  7. Topics
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We are analyzing https://link.springer.com/article/10.1186/s12943-018-0820-2.

Title:
The lncRNA MACC1-AS1 promotes gastric cancer cell metabolic plasticity via AMPK/Lin28 mediated mRNA stability of MACC1 | Molecular Cancer
Description:
Background Metabolic plasticity has been increasingly thought to be a determinant of tumor growth and metastasis. MACC1, a transcriptional regulator of MET, was recognized as an oncogene in gastric cancer (GC); however, its transcriptional or post-translational regulation was not clear. We previously reported the metabolic role of MACC1 in glycolysis to promote GC progression. MACC1-AS1 is the antisense lncRNA of MACC1, yet its function was previously unknown. Methods We profiled and analyzed the expression of MACC1-AS1 utilizing the TCGA database as well as in situ hybridization using 123 pairs of GC tissues and matched adjacent normal gastric mucosa tissues (ANTs). The biological role of MACC1-AS1 in cell growth and metastasis was determined by performing in vitro and in vivo functional experiments. Glycolysis and antioxidant capabilities were assayed to examine its metabolic function. Further, the specific regulatory effect of MACC1-AS1 on MACC1 was explored transcriptionally and post-transcriptionally. Results MACC1-AS1 was shown to be expressed significantly higher in GC tissues than in ANTs, which predicted poor prognosis in GC patients. MACC1-AS1 promoted GC cell proliferation and inhibited cell apoptosis under metabolic stress. Mechanistically, MACC1-AS1 stabilized MACC1 mRNA and post-transcriptionally augmented MACC1 expression. Further, MACC1-AS1 was shown to mediate metabolic plasticity through MACC1 upregulation and subsequent enhanced glycolysis and anti-oxidative capabilities, and this was suggested to be coordinated by the AMPK/Lin28 pathway. Conclusions Elevated expression of MACC1-AS1 in gastric cancer tissues is linked to poor prognosis and promotes malignant phenotype upon cancer cells. MACC1-AS1 is elevated under metabolic stress and facilitates metabolic plasticity by promoting MACC1 expression through mRNA stabilization. Our study implicates lncRNA MACC1-AS1 as a valuable biomarker for GC diagnosis and prognosis.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Education
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Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

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Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


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Keywords {🔍}

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Topics {✒️}

plenti-ef1a-luc-f2a-puro-cmv-macc1-as1 constructs plkd-u6-shrna-ef1a-luc-f2a-puro-macc1 ros scavenger n-acetyl-l-cysteine macc1-as1/macc1 axis functioned large-scale clip-seq data pet-ct image analysis article download pdf macc1-as1 overexpression group n-acetyl-l-cysteine lin28-mediated post-transcriptional regulation macc1-as1 promotes migration macc1-as1 expression mitigated profile gene expression cyclohexamide-induced macc1 degradation macc1-as1 promoted migration sds–polyacrylamide gel electrophoresis serial saline-sodium citrate hgf/c-met axis macc1-as1 regulates glycolysis macc1-as1 partially abrogated macc1-as1 positive group macc1-as1 promoted atp macc1-as1 promoted glutathione macc1-as1 negative group pgl3-macc1-3′utr vector kaplan–meier analysis showed macc1-as1 enhances glycolysis macc1-as1 promoted hk synthetic sequence-scrambled sirna macc1-as1 overexpression groups biotin-labeled macc1-as1 target hgf/c-met showed macc1-as1 enhanced hgf/c-met inhibitors measure macc1-as1 expression antisense macc1-as1 sequence forming rna-rna complexes firefly/renilla luciferase assays nadp+/nadph quantification kit protein–rna interaction networks macc1-as1 partially reversed high macc1-as1 expression macc1 shrna-induced glut1 reduced disease-free survival ampk inhibition-induced suppression fluorescent-tagged secondary antibody promotes metabolic plasticity glucose deprivation-induced expression gssg/gsh ratios decreased gsh/gssg quantification kit

Schema {🗺️}

WebPage:
      mainEntity:
         headline:The lncRNA MACC1-AS1 promotes gastric cancer cell metabolic plasticity via AMPK/Lin28 mediated mRNA stability of MACC1
         description:Metabolic plasticity has been increasingly thought to be a determinant of tumor growth and metastasis. MACC1, a transcriptional regulator of MET, was recognized as an oncogene in gastric cancer (GC); however, its transcriptional or post-translational regulation was not clear. We previously reported the metabolic role of MACC1 in glycolysis to promote GC progression. MACC1-AS1 is the antisense lncRNA of MACC1, yet its function was previously unknown. We profiled and analyzed the expression of MACC1-AS1 utilizing the TCGA database as well as in situ hybridization using 123 pairs of GC tissues and matched adjacent normal gastric mucosa tissues (ANTs). The biological role of MACC1-AS1 in cell growth and metastasis was determined by performing in vitro and in vivo functional experiments. Glycolysis and antioxidant capabilities were assayed to examine its metabolic function. Further, the specific regulatory effect of MACC1-AS1 on MACC1 was explored transcriptionally and post-transcriptionally. MACC1-AS1 was shown to be expressed significantly higher in GC tissues than in ANTs, which predicted poor prognosis in GC patients. MACC1-AS1 promoted GC cell proliferation and inhibited cell apoptosis under metabolic stress. Mechanistically, MACC1-AS1 stabilized MACC1 mRNA and post-transcriptionally augmented MACC1 expression. Further, MACC1-AS1 was shown to mediate metabolic plasticity through MACC1 upregulation and subsequent enhanced glycolysis and anti-oxidative capabilities, and this was suggested to be coordinated by the AMPK/Lin28 pathway. Elevated expression of MACC1-AS1 in gastric cancer tissues is linked to poor prognosis and promotes malignant phenotype upon cancer cells. MACC1-AS1 is elevated under metabolic stress and facilitates metabolic plasticity by promoting MACC1 expression through mRNA stabilization. Our study implicates lncRNA MACC1-AS1 as a valuable biomarker for GC diagnosis and prognosis.
         datePublished:2018-03-06T00:00:00Z
         dateModified:2018-03-06T00:00:00Z
         pageStart:1
         pageEnd:16
         license:http://creativecommons.org/publicdomain/zero/1.0/
         sameAs:https://doi.org/10.1186/s12943-018-0820-2
         keywords:
            MACC1-AS1
            MACC1
            Long non-coding RNA
            Gastric cancer
            Metabolic plasticity
            mRNA stability
            Cancer Research
            Oncology
         image:
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         isPartOf:
            name:Molecular Cancer
            issn:
               1476-4598
            volumeNumber:17
            type:
               Periodical
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            name:BioMed Central
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               type:ImageObject
            type:Organization
         author:
               name:Yang Zhao
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
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               name:Yajing Liu
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Li Lin
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Qiong Huang
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Wanming He
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Shuyi Zhang
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Shumin Dong
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Zhaowei Wen
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jinjun Rao
               affiliation:
                     name:Southern Medical University
                     address:
                        name:Key laboratory of new drug screening of Guangdong province, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Wangjun Liao
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Min Shi
               affiliation:
                     name:Nanfang Hospital, Southern Medical University
                     address:
                        name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:The lncRNA MACC1-AS1 promotes gastric cancer cell metabolic plasticity via AMPK/Lin28 mediated mRNA stability of MACC1
      description:Metabolic plasticity has been increasingly thought to be a determinant of tumor growth and metastasis. MACC1, a transcriptional regulator of MET, was recognized as an oncogene in gastric cancer (GC); however, its transcriptional or post-translational regulation was not clear. We previously reported the metabolic role of MACC1 in glycolysis to promote GC progression. MACC1-AS1 is the antisense lncRNA of MACC1, yet its function was previously unknown. We profiled and analyzed the expression of MACC1-AS1 utilizing the TCGA database as well as in situ hybridization using 123 pairs of GC tissues and matched adjacent normal gastric mucosa tissues (ANTs). The biological role of MACC1-AS1 in cell growth and metastasis was determined by performing in vitro and in vivo functional experiments. Glycolysis and antioxidant capabilities were assayed to examine its metabolic function. Further, the specific regulatory effect of MACC1-AS1 on MACC1 was explored transcriptionally and post-transcriptionally. MACC1-AS1 was shown to be expressed significantly higher in GC tissues than in ANTs, which predicted poor prognosis in GC patients. MACC1-AS1 promoted GC cell proliferation and inhibited cell apoptosis under metabolic stress. Mechanistically, MACC1-AS1 stabilized MACC1 mRNA and post-transcriptionally augmented MACC1 expression. Further, MACC1-AS1 was shown to mediate metabolic plasticity through MACC1 upregulation and subsequent enhanced glycolysis and anti-oxidative capabilities, and this was suggested to be coordinated by the AMPK/Lin28 pathway. Elevated expression of MACC1-AS1 in gastric cancer tissues is linked to poor prognosis and promotes malignant phenotype upon cancer cells. MACC1-AS1 is elevated under metabolic stress and facilitates metabolic plasticity by promoting MACC1 expression through mRNA stabilization. Our study implicates lncRNA MACC1-AS1 as a valuable biomarker for GC diagnosis and prognosis.
      datePublished:2018-03-06T00:00:00Z
      dateModified:2018-03-06T00:00:00Z
      pageStart:1
      pageEnd:16
      license:http://creativecommons.org/publicdomain/zero/1.0/
      sameAs:https://doi.org/10.1186/s12943-018-0820-2
      keywords:
         MACC1-AS1
         MACC1
         Long non-coding RNA
         Gastric cancer
         Metabolic plasticity
         mRNA stability
         Cancer Research
         Oncology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12943-018-0820-2/MediaObjects/12943_2018_820_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1186%2Fs12943-018-0820-2/MediaObjects/12943_2018_820_Fig2_HTML.gif
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      isPartOf:
         name:Molecular Cancer
         issn:
            1476-4598
         volumeNumber:17
         type:
            Periodical
            PublicationVolume
      publisher:
         name:BioMed Central
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Yang Zhao
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yajing Liu
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Li Lin
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Qiong Huang
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Wanming He
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Shuyi Zhang
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Shumin Dong
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Zhaowei Wen
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jinjun Rao
            affiliation:
                  name:Southern Medical University
                  address:
                     name:Key laboratory of new drug screening of Guangdong province, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Wangjun Liao
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Min Shi
            affiliation:
                  name:Nanfang Hospital, Southern Medical University
                  address:
                     name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Molecular Cancer
      issn:
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      name:BioMed Central
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      name:Nanfang Hospital, Southern Medical University
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         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
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      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Southern Medical University
      address:
         name:Key laboratory of new drug screening of Guangdong province, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
      name:Nanfang Hospital, Southern Medical University
      address:
         name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
         type:PostalAddress
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      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
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      name:Yang Zhao
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Yajing Liu
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Li Lin
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Qiong Huang
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Wanming He
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Shuyi Zhang
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Shumin Dong
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Zhaowei Wen
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Jinjun Rao
      affiliation:
            name:Southern Medical University
            address:
               name:Key laboratory of new drug screening of Guangdong province, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Wangjun Liao
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      name:Min Shi
      affiliation:
            name:Nanfang Hospital, Southern Medical University
            address:
               name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Key laboratory of new drug screening of Guangdong province, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China
      name:Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China

External Links {🔗}(194)

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