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Title:
Tumor-associated copy number changes in the circulation of patients with prostate cancer identified through whole-genome sequencing | Genome Medicine
Description:
Background Patients with prostate cancer may present with metastatic or recurrent disease despite initial curative treatment. The propensity of metastatic prostate cancer to spread to the bone has limited repeated sampling of tumor deposits. Hence, considerably less is understood about this lethal metastatic disease, as it is not commonly studied. Here we explored whole-genome sequencing of plasma DNA to scan the tumor genomes of these patients non-invasively. Methods We wanted to make whole-genome analysis from plasma DNA amenable to clinical routine applications and developed an approach based on a benchtop high-throughput platform, that is, Illuminas MiSeq instrument. We performed whole-genome sequencing from plasma at a shallow sequencing depth to establish a genome-wide copy number profile of the tumor at low costs within 2 days. In parallel, we sequenced a panel of 55 high-interest genes and 38 introns with frequent fusion breakpoints such as the TMPRSS2-ERG fusion with high coverage. After intensive testing of our approach with samples from 25 individuals without cancer we analyzed 13 plasma samples derived from five patients with castration resistant (CRPC) and four patients with castration sensitive prostate cancer (CSPC). Results The genome-wide profiling in the plasma of our patients revealed multiple copy number aberrations including those previously reported in prostate tumors, such as losses in 8p and gains in 8q. High-level copy number gains in the AR locus were observed in patients with CRPC but not with CSPC disease. We identified the TMPRSS2-ERG rearrangement associated 3-Mbp deletion on chromosome 21 and found corresponding fusion plasma fragments in these cases. In an index case multiregional sequencing of the primary tumor identified different copy number changes in each sector, suggesting multifocal disease. Our plasma analyses of this index case, performed 13 years after resection of the primary tumor, revealed novel chromosomal rearrangements, which were stable in serial plasma analyses over a 9-month period, which is consistent with the presence of one metastatic clone. Conclusions The genomic landscape of prostate cancer can be established by non-invasive means from plasma DNA. Our approach provides specific genomic signatures within 2 days which may therefore serve as
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cancer, plasma, prostate, pubmed, sequencing, dna, article, crpc, tumor, patients, google, scholar, cspc, cas, samples, number, copy, analysis, zscores, approach, central, figure, analyses, genome, disease, file, plasmaseq, data, approximately, regions, performed, zscore, genomewide, additional, metastatic, genes, mbp, controls, wholegenome, previously, chromosome, cases, mutations, obtained, blood, reads, clinical, panel, cells, identified,
Topics {βοΈ}
org/cancergenomics/prostate/data/mskcc_pca_segmented_copy-number uk/genetics/cgp/cosmic/ http increased genome-wide z-score gc-corrected read-count ratios tmprss2-erg-negative prostate cancers article download pdf tmprss2-erg-positive prostate cancers segmented dna-copy-number data benchtop high-throughput platform calculated gene-specific z-scores theresa benezeder androgen-receptor signaling axis european genome-phenome archive genome-wide z-score genome-wide z- score genome-wide z-scores z-score calculation microvortex-generating herringbone-chip tumor-specific copy number array-cgh profiles show blood copy-number signatures reliable copy-number measurements gene-specific z-scores distinguishing tmprss2-erg-positive akt-inactivating phosphatase phlpp1 androgen receptor gene cross-validated z-scores de bono js cell-free nucleic acids ar copy-number status castration-resistant prostate cancer androgen receptor signaling copy number profiles high-throughput sequencing data copy number profile array-cgh array-cgh par-masked hg19 genome simulated copy-number analyses cancer-specific genomic rearrangements published ctc-based studies copy number aberrations ets gene fusions large-scale sequencing projects counted high-quality alignments open-source package calculated 'segmental z-scores' clinic search search array-cgh profiles array-cgh profile identical copy number
Questions {β}
- Albertsen PC: Treatment of localized prostate cancer: when is active surveillance appropriate?
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headline:Tumor-associated copy number changes in the circulation of patients with prostate cancer identified through whole-genome sequencing
description:Patients with prostate cancer may present with metastatic or recurrent disease despite initial curative treatment. The propensity of metastatic prostate cancer to spread to the bone has limited repeated sampling of tumor deposits. Hence, considerably less is understood about this lethal metastatic disease, as it is not commonly studied. Here we explored whole-genome sequencing of plasma DNA to scan the tumor genomes of these patients non-invasively. We wanted to make whole-genome analysis from plasma DNA amenable to clinical routine applications and developed an approach based on a benchtop high-throughput platform, that is, Illuminas MiSeq instrument. We performed whole-genome sequencing from plasma at a shallow sequencing depth to establish a genome-wide copy number profile of the tumor at low costs within 2 days. In parallel, we sequenced a panel of 55 high-interest genes and 38 introns with frequent fusion breakpoints such as the TMPRSS2-ERG fusion with high coverage. After intensive testing of our approach with samples from 25 individuals without cancer we analyzed 13 plasma samples derived from five patients with castration resistant (CRPC) and four patients with castration sensitive prostate cancer (CSPC). The genome-wide profiling in the plasma of our patients revealed multiple copy number aberrations including those previously reported in prostate tumors, such as losses in 8p and gains in 8q. High-level copy number gains in the AR locus were observed in patients with CRPC but not with CSPC disease. We identified the TMPRSS2-ERG rearrangement associated 3-Mbp deletion on chromosome 21 and found corresponding fusion plasma fragments in these cases. In an index case multiregional sequencing of the primary tumor identified different copy number changes in each sector, suggesting multifocal disease. Our plasma analyses of this index case, performed 13 years after resection of the primary tumor, revealed novel chromosomal rearrangements, which were stable in serial plasma analyses over a 9-month period, which is consistent with the presence of one metastatic clone. The genomic landscape of prostate cancer can be established by non-invasive means from plasma DNA. Our approach provides specific genomic signatures within 2 days which may therefore serve as 'liquid biopsy'.
datePublished:2013-04-05T00:00:00Z
dateModified:2013-04-05T00:00:00Z
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keywords:
Prostate Cancer
Androgen Receptor
Metastatic Prostate Cancer
Copy Number Change
Copy Number Aberration
Human Genetics
Metabolomics
Bioinformatics
Medicine/Public Health
general
Cancer Research
Systems Biology
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headline:Tumor-associated copy number changes in the circulation of patients with prostate cancer identified through whole-genome sequencing
description:Patients with prostate cancer may present with metastatic or recurrent disease despite initial curative treatment. The propensity of metastatic prostate cancer to spread to the bone has limited repeated sampling of tumor deposits. Hence, considerably less is understood about this lethal metastatic disease, as it is not commonly studied. Here we explored whole-genome sequencing of plasma DNA to scan the tumor genomes of these patients non-invasively. We wanted to make whole-genome analysis from plasma DNA amenable to clinical routine applications and developed an approach based on a benchtop high-throughput platform, that is, Illuminas MiSeq instrument. We performed whole-genome sequencing from plasma at a shallow sequencing depth to establish a genome-wide copy number profile of the tumor at low costs within 2 days. In parallel, we sequenced a panel of 55 high-interest genes and 38 introns with frequent fusion breakpoints such as the TMPRSS2-ERG fusion with high coverage. After intensive testing of our approach with samples from 25 individuals without cancer we analyzed 13 plasma samples derived from five patients with castration resistant (CRPC) and four patients with castration sensitive prostate cancer (CSPC). The genome-wide profiling in the plasma of our patients revealed multiple copy number aberrations including those previously reported in prostate tumors, such as losses in 8p and gains in 8q. High-level copy number gains in the AR locus were observed in patients with CRPC but not with CSPC disease. We identified the TMPRSS2-ERG rearrangement associated 3-Mbp deletion on chromosome 21 and found corresponding fusion plasma fragments in these cases. In an index case multiregional sequencing of the primary tumor identified different copy number changes in each sector, suggesting multifocal disease. Our plasma analyses of this index case, performed 13 years after resection of the primary tumor, revealed novel chromosomal rearrangements, which were stable in serial plasma analyses over a 9-month period, which is consistent with the presence of one metastatic clone. The genomic landscape of prostate cancer can be established by non-invasive means from plasma DNA. Our approach provides specific genomic signatures within 2 days which may therefore serve as 'liquid biopsy'.
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dateModified:2013-04-05T00:00:00Z
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keywords:
Prostate Cancer
Androgen Receptor
Metastatic Prostate Cancer
Copy Number Change
Copy Number Aberration
Human Genetics
Metabolomics
Bioinformatics
Medicine/Public Health
general
Cancer Research
Systems Biology
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type:PostalAddress
type:Organization
name:Sabine Riethdorf
affiliation:
name:Institute of Tumor Biology, University Medical Center Hamburg Eppendorf
address:
name:Institute of Tumor Biology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
type:PostalAddress
type:Organization
name:Klaus Pantel
affiliation:
name:Institute of Tumor Biology, University Medical Center Hamburg Eppendorf
address:
name:Institute of Tumor Biology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
type:PostalAddress
type:Organization
name:Hellmut Samonigg
affiliation:
name:Medical University of Graz
address:
name:Division of Oncology, Medical University of Graz, Graz, Austria
type:PostalAddress
type:Organization
name:Gerald Hoefler
affiliation:
name:Institute of Pathology, Medical University of Graz
address:
name:Institute of Pathology, Medical University of Graz, Graz, Austria
type:PostalAddress
type:Organization
name:Herbert Augustin
affiliation:
name:Medical University of Graz
address:
name:Department of Urology, Medical University of Graz, Graz, Austria
type:PostalAddress
type:Organization
name:Jochen B Geigl
affiliation:
name:Institute of Human Genetics, Medical University of Graz
address:
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
type:PostalAddress
type:Organization
email:[email protected]
name:Michael R Speicher
affiliation:
name:Institute of Human Genetics, Medical University of Graz
address:
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
type:PostalAddress
type:Organization
email:[email protected]
PostalAddress:
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
name:Department of Urology, Medical University of Graz, Graz, Austria
name:Institute for Medical Informatics, Statistics and Documentation, Medical University of Graz, Graz, Austria
name:Department of Urology, Medical University of Graz, Graz, Austria
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
name:Institute of Pathology, Medical University of Graz, Graz, Austria
name:Division of Oncology, Medical University of Graz, Graz, Austria
name:Division of Oncology, Medical University of Graz, Graz, Austria
name:Department of Obstetrics and Gynecology, Medical University of Graz, Graz, Austria
name:Institute of Tumor Biology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
name:Institute of Tumor Biology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
name:Division of Oncology, Medical University of Graz, Graz, Austria
name:Institute of Pathology, Medical University of Graz, Graz, Austria
name:Department of Urology, Medical University of Graz, Graz, Austria
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
name:Institute of Human Genetics, Medical University of Graz, Graz, Austria
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