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Title:
Analysis of cell-based RNAi screens | Genome Biology
Description:
RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS ( http://www.dkfz.de/signaling/cellHTS ). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.
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data, analysis, file, article, rnai, google, scholar, figure, plate, pubmed, package, cellhts, cas, cellbased, screens, screening, screen, table, quality, experiment, provided, information, shown, plates, experiments, full, assay, plots, gene, dsrnas, nature, cell, genes, set, dataset, signal, median, biological, size, values, files, normalization, controls, shows, additional, software, results, original, rna, phenotype,
Topics {✒️}
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headline:Analysis of cell-based RNAi screens
description:RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (
http://www.dkfz.de/signaling/cellHTS
). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.
datePublished:2006-07-25T00:00:00Z
dateModified:2006-07-25T00:00:00Z
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Quality Metrics
Median Absolute Deviation
RNAi Experiment
RNAi Screen
Animal Genetics and Genomics
Human Genetics
Plant Genetics and Genomics
Microbial Genetics and Genomics
Bioinformatics
Evolutionary Biology
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headline:Analysis of cell-based RNAi screens
description:RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (
http://www.dkfz.de/signaling/cellHTS
). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.
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Quality Metrics
Median Absolute Deviation
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Animal Genetics and Genomics
Human Genetics
Plant Genetics and Genomics
Microbial Genetics and Genomics
Bioinformatics
Evolutionary Biology
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