
NCBI . NLM . NIH . GOV {
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Title[redir]:
Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways - PMC
Description:
We demonstrate the efficacy of double-stranded RNA-mediated interference (RNAi) of gene expression in generating βknock-outβ phenotypes for specific proteins in several Drosophila cell lines. We prove the applicability of this technique for studying ...
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Keywords {π}
dsrna, dshpx, doi, cells, cell, dack, drosophila, rnai, protein, google, scholar, pubmed, insulin, proteins, expression, dock, culture, kinase, media, fig, results, signaling, erka, dsrnas, western, pmc, signal, transduction, pathway, dsor, activation, analysis, levels, biol, production, rna, lines, gene, specific, phosphorylation, effect, domain, days, activity, daktpkb, antibodies, nlm, pathways, article, tyrosine,
Topics {βοΈ}
extracellular signal-regulated kinase double-stranded rna-mediated interference double-stranded rna interference mitogen-activated protein kinase double-stranded rna phox homology domain central phox homology phosphoinositol-3 kinase insulin receptor substrate mediated interference px irs anti-sense rna experiments 4g10 anti-phosphotyrosine antibody identified downstream target/substrate mammalian insulin receptor vitro kinase assays phosphoinositide-dependent kinase drosophila akt signal transduction cascades regulate dakt/pkb activity /protein kinase pmc beta search 6xhis-tagged-sh2 domain 6xhis-tagged sh2 domain akt/pkb dakt/pkb activity measured tyrosine kinase nh2-terminal sh3 domain trisphosphate [pi hydrolyze pi cellular pi specific rna drosophila ack mapk pathway rna processing kinase upstream publisher site pdf insulin signaling pathway pi3k branches drosophila cell lines activate dakt/pkb 6xhis-tagged version dakt/pkb activity specific interference high throughput screens insulin signaling cascade effector/substrate drosophila cell culture dock protein levels
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