We are analyzing https://link.springer.com/article/10.1186/bcr3593.

Title:
New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells | Breast Cancer Research
Description:
Introduction Parity-identified mammary epithelial cells (PI-MECs) are an interesting cellular subset because they survive involution and are a presumptive target for transformation by human epidermal growth factor receptor 2 (HER2)/neu in mammary tumors. Depending on the type of assay, PI-MECs have been designated lobule-restricted progenitors or multipotent stem/progenitor cells. PI-MECs were reported to be part of the basal population of mammary epithelium based on flow cytometry. We investigated the cellular identity and lineage potential of PI-MECs in intact mammary glands. Methods We performed a quantitative and qualitative analysis of the contribution of PI-MECs to mammary epithelial cell lineages in pregnant and involuted mammary glands by immunohistochemistry, fluorescence-activated cells sorting (FACS), and quantitative polymerase chain reaction. PI-MECs were labeled by the activation of Whey Acidic Protein (WAP)-Cre during pregnancy that results in permanent expression of yellow fluorescent protein. Results After involution, PI-MECs are present exclusively in the luminal layer of mammary ducts. During pregnancy, PI-MECs contribute to the luminal layer but not the basal layer of alveolar lobules. Strikingly, whereas all luminal estrogen receptor (ER)-negative cells in an alveolus can be derived from PI-MECs, the alveolar ER-positive cells are unlabeled and reminiscent of Notch2-traced L cells. Notably, we observed a significant population of unlabeled alveolar progenitors that resemble PI-MECs based on transcriptional and histological analysis. Conclusions Our demonstration that PI-MECs are luminal cells underscores that not only basal cells display multi-lineage potential in transplantation assays. However, the lineage potential of PI-MECs in unperturbed mammary glands is remarkably restricted to luminal ER-negative cells of the secretory alveolar lineage. The identification of an unlabeled but functionally similar population of luminal alveolar progenitor cells raises the question of whether PI-MECs are a unique population or the result of stochastic labeling. Interestingly, even when all luminal ER-negative cells of an alveolus are PI-MEC-derived, the basal cells and hormone-sensing cells are derived from a different source, indicating that cooperative outgrowth of cells from different lineages is common in alveologenesis.
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Keywords {🔍}

cells, mammary, pimecs, cell, luminal, figure, alveolar, pregnancy, epithelial, alveoli, basal, yfppos, pubmed, population, yfp, article, glands, progenitor, expression, file, google, scholar, lineage, mice, hormonesensing, days, gland, additional, day, cas, stem, wap, yfpneg, facs, analysis, fluorescent, protein, populations, unlabeled, data, parityidentified, receptor, involuted, yellow, reporter, potential, labeled, red, central, layer,

Topics {✒️}

rosa26-lox-stop-lox-yfp locus amplify rosa26-lox-stop-lox-yellow fluorescent protein wnt/β-catenin-responsive stem cells rosa26-lox-stop-lox-yfp mice [24] rosa26-lox-stop-lox-yfp rt rosa26-lox-stop-lox-yfp cf633nm-coupled donkey anti-rat alexa488-coupled goat anti-rabbit alexa568-coupled goat anti-rabbit alexa488-coupled goat anti-mouse alexa568-coupled goat anti-mouse rosa-lsl-yfp mammary glands multipotent stem/progenitor cells er-positive hormone-sensing cell rosa-lsl-yfp animals showed yellow fluorescent protein-negative er-positive hormone-sensing cells article download pdf yellow fluorescent protein-positive figure 6e versus figure 4a rosa-lsl-yfp transgenes whey acidic protein partial-versus-totally labeled alveoli rosa-lsl-yfp locus steroid receptor-positive cells single-stain antibody controls rosa-lsl-yfp glands fluorescence-activated cell sorting rosa-lsl-yfp strain rosa-lsl-yfp mice anti-rat compensation beads designated lobule-restricted progenitors bio-rad iselect kit axin2-traced cells remained hormone-sensing cells lose hormone-sensing cells play generates hormone-sensing cells bipotent lobule-restricted progenitor rosa-lsl-yfp females fluorescence-activated cells sorting related subjects multipotent mammary progenitor cell stem cell single stem cell independent gland-autonomous effects mmtv-neu transgenic mice hormone-sensing cell population includes er-positive cells wap-cre/yfp labeling system her2/neu-driven tumorigenesis [12–14]

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