We are analyzing https://link.springer.com/article/10.1186/bcr1538.

Title:
Estrogen receptor-α and progesterone receptor are expressed in label-retaining mammary epithelial cells that divide asymmetrically and retain their template DNA strands | Breast Cancer Research
Description:
Introduction Stem cells of somatic tissues are hypothesized to protect themselves from mutation and cancer risk through a process of selective segregation of their template DNA strands during asymmetric division. Mouse mammary epithelium contains label-retaining epithelial cells that divide asymmetrically and retain their template DNA. Method Immunohistochemistry was used in murine mammary glands that had been labeled with [3H]thymidine during allometric growth to investigate the co-expression of DNA label retention and estrogen receptor (ER)-α or progesterone receptor (PR). Using the same methods, we investigated the co-localization of [3H]thymidine and ER-α or PR in mammary tissue from mice that had received treatment with estrogen, progesterone, and prolactin subsequent to a long chase period to identify label-retaining cells. Results Label-retaining epithelial cells (LRECs) comprised approximately 2.0% of the entire mammary epithelium. ER-α-positive and PR-positive cells represented about 30–40% of the LREC subpopulation. Administration of estrogen, progesterone, and prolactin altered the percentage of LRECs expressing ER-α. Conclusion The results presented here support the premise that there is a subpopulation of LRECs in the murine mammary gland that is positive for ER-α and/or PR. This suggests that certain mammary LRECs (potentially stem cells) remain stably positive for these receptors, raising the possibility that LRECs comprise a hierarchy of asymmetrically cycling mammary stem/progenitor cells that are distinguished by the presence or absence of nuclear steroid receptor expression.
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Keywords {🔍}

cells, mammary, lrecs, epithelial, erα, hthymidine, positive, receptor, estrogen, cell, article, progesterone, labeled, epithelium, erαpositive, estradiol, figure, labelretaining, dna, stem, label, tissue, growth, pubmed, expression, cycle, group, received, original, day, google, scholar, cancer, gland, days, authors, mouse, hormone, cas, mice, treatment, animals, data, breast, asymmetrically, chase, period, prpositive, percentage, receptors,

Topics {✒️}

er-α-positive long-label-retaining cells long-label-retaining stem/progenitors cells er-α-negative label-retaining cells er-α-positive label-retaining cells long-label-retaining mammary cells tgf-β1-mediated restraint appeared er-α-negative/pr-negative lrecs er-α-positive/pr-positive lrecs label-retaining stromal cells label-retaining epithelial cells label-retaining epithelial cell full size image identify label-retaining cells numerous er-α-negative stem/progenitor cell expansion nuclear label [3h]thymidine article download pdf er-negative cells initiating steroid receptor-positive cells er-α-negative cells human breast cancer constitutively active tgf-β1 periductal stromal cells er-α positive rose er-α-positive cells breast cancer res human breast progenitors giving [3h]thymidine label pr-positive epithelial cells mitogen-activated protein kinase long label retaining stem/progenitor cells epithelial stem cells epithelial stem cell initial [3h]thymidine injections transforming growth factor-beta1 labeled er-α-positive mouse mammary gland label-retaining cells er-α-positive staining lrecs expressing er-α potentially stem cells estrogen receptor-alpha increased er-α expression er-α-positive lrecs [3h]thymidine-labeled cells adult mammary tissues final [3h]thymidine injection full access mammary epithelial cells

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         headline:Estrogen receptor-α and progesterone receptor are expressed in label-retaining mammary epithelial cells that divide asymmetrically and retain their template DNA strands
         description:Stem cells of somatic tissues are hypothesized to protect themselves from mutation and cancer risk through a process of selective segregation of their template DNA strands during asymmetric division. Mouse mammary epithelium contains label-retaining epithelial cells that divide asymmetrically and retain their template DNA. Immunohistochemistry was used in murine mammary glands that had been labeled with [3H]thymidine during allometric growth to investigate the co-expression of DNA label retention and estrogen receptor (ER)-α or progesterone receptor (PR). Using the same methods, we investigated the co-localization of [3H]thymidine and ER-α or PR in mammary tissue from mice that had received treatment with estrogen, progesterone, and prolactin subsequent to a long chase period to identify label-retaining cells. Label-retaining epithelial cells (LRECs) comprised approximately 2.0% of the entire mammary epithelium. ER-α-positive and PR-positive cells represented about 30–40% of the LREC subpopulation. Administration of estrogen, progesterone, and prolactin altered the percentage of LRECs expressing ER-α. The results presented here support the premise that there is a subpopulation of LRECs in the murine mammary gland that is positive for ER-α and/or PR. This suggests that certain mammary LRECs (potentially stem cells) remain stably positive for these receptors, raising the possibility that LRECs comprise a hierarchy of asymmetrically cycling mammary stem/progenitor cells that are distinguished by the presence or absence of nuclear steroid receptor expression.
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            Progesterone Receptor
            Mammary Epithelial Cell
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            Progesterone Receptor Expression
            Cancer Research
            Oncology
            Surgical Oncology
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      headline:Estrogen receptor-α and progesterone receptor are expressed in label-retaining mammary epithelial cells that divide asymmetrically and retain their template DNA strands
      description:Stem cells of somatic tissues are hypothesized to protect themselves from mutation and cancer risk through a process of selective segregation of their template DNA strands during asymmetric division. Mouse mammary epithelium contains label-retaining epithelial cells that divide asymmetrically and retain their template DNA. Immunohistochemistry was used in murine mammary glands that had been labeled with [3H]thymidine during allometric growth to investigate the co-expression of DNA label retention and estrogen receptor (ER)-α or progesterone receptor (PR). Using the same methods, we investigated the co-localization of [3H]thymidine and ER-α or PR in mammary tissue from mice that had received treatment with estrogen, progesterone, and prolactin subsequent to a long chase period to identify label-retaining cells. Label-retaining epithelial cells (LRECs) comprised approximately 2.0% of the entire mammary epithelium. ER-α-positive and PR-positive cells represented about 30–40% of the LREC subpopulation. Administration of estrogen, progesterone, and prolactin altered the percentage of LRECs expressing ER-α. The results presented here support the premise that there is a subpopulation of LRECs in the murine mammary gland that is positive for ER-α and/or PR. This suggests that certain mammary LRECs (potentially stem cells) remain stably positive for these receptors, raising the possibility that LRECs comprise a hierarchy of asymmetrically cycling mammary stem/progenitor cells that are distinguished by the presence or absence of nuclear steroid receptor expression.
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         Mammary Gland
         Progesterone Receptor
         Mammary Epithelial Cell
         Mammary Epithelium
         Progesterone Receptor Expression
         Cancer Research
         Oncology
         Surgical Oncology
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