We are analyzing https://link.springer.com/article/10.1186/1471-2172-3-7.

Title:
IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype | BMC Immunology
Description:
Background "Alternatively-activated" macrophages are found in Th2-mediated inflammatory settings such as nematode infection and allergic pulmonary inflammation. Due in part to a lack of markers, these cells have not been well characterized in vivo and their function remains unknown. Results We have used murine macrophages elicited by nematode infection (NeMφ) as a source of in vivo derived alternatively activated macrophages. Using three distinct yet complementary molecular approaches we have established a gene expression profile of alternatively activated macrophages and identified macrophage genes that are regulated in vivo by IL-4. First, genes abundantly expressed were identified by an expressed sequence tag strategy. Second, an array of 1176 known mouse genes was screened for differential expression between NeMφ from wild type or IL-4 deficient mice. Third, a subtractive library was screened to identify novel IL-4 dependent macrophage genes. Differential expression was confirmed by real time RT-PCR analysis. Conclusions Our data demonstrate that alternatively activated macrophages generated in vivo have a gene expression profile distinct from any macrophage population described to date. Several of the genes we identified, including those most abundantly expressed, have not previously been associated with macrophages and thus this study provides unique new information regarding the phenotype of macrophages found in Th2-mediated, chronic inflammatory settings. Our data also provide additional in vivo evidence for parallels between the inflammatory processes involved in nematode infection and allergy.
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Keywords {🔍}

macrophages, expression, genes, pubmed, google, scholar, article, cas, mice, gene, nemφ, analysis, cells, infection, macrophage, cdna, vivo, activated, expressed, arginase, dependent, activation, pcr, alternatively, library, purified, array, peritoneal, journal, nematode, mouse, cytokines, identified, est, figure, immunology, found, malayi, inflammation, murine, abundantly, parasite, subtractive, fizzrelmα, sequences, function, inflammatory, results, products, upregulated,

Topics {✒️}

regulate neuro-immunological cross-talk gene expression profiles lightcycler-dna sybr green quantitative real-time pcr cysteine-rich secreted protein pcmv-script plasmid vector uk/seq_tables/macrophage/macro surgically implanted intra-peritoneally real-time pcr analysis nitric oxide synthase-2 article download pdf major acute-phase reactant peritoneal exudate cells open access license eosinophil chemotactic factor lipopolysaccharide-induced proteins secreted th2-mediated inflammatory settings john parkinson magnetic bead sorting ova-induced pulmonary inflammation lps-stimulated human monocytes promoting th2-mediated pathology stat6 dependent manner cysteine rich gene pro-inflammatory th1 pathways growth factor ia authors’ original file real-time pcr privacy choices/manage cookies gene expression profile la flamme ac classically-activated macrophage fizz/relm gene family peritoneal exudate semi-quantitative pcr eosinophil chemotactic cytokine th1/th2-regulated expression semiquantitative rt-pcr constitutive nuclear factor macrophage population cell-binding characteristics article loke infected il-4-deficient mice rt-pcr analysis il-4-deficient nemφ relative fizz1/relm-α suggests alternatively activated macrophages nematode elicited macrophages f4/80+ macrophages derived full size image

Schema {🗺️}

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         description:"Alternatively-activated" macrophages are found in Th2-mediated inflammatory settings such as nematode infection and allergic pulmonary inflammation. Due in part to a lack of markers, these cells have not been well characterized in vivo and their function remains unknown. We have used murine macrophages elicited by nematode infection (NeMφ) as a source of in vivo derived alternatively activated macrophages. Using three distinct yet complementary molecular approaches we have established a gene expression profile of alternatively activated macrophages and identified macrophage genes that are regulated in vivo by IL-4. First, genes abundantly expressed were identified by an expressed sequence tag strategy. Second, an array of 1176 known mouse genes was screened for differential expression between NeMφ from wild type or IL-4 deficient mice. Third, a subtractive library was screened to identify novel IL-4 dependent macrophage genes. Differential expression was confirmed by real time RT-PCR analysis. Our data demonstrate that alternatively activated macrophages generated in vivo have a gene expression profile distinct from any macrophage population described to date. Several of the genes we identified, including those most abundantly expressed, have not previously been associated with macrophages and thus this study provides unique new information regarding the phenotype of macrophages found in Th2-mediated, chronic inflammatory settings. Our data also provide additional in vivo evidence for parallels between the inflammatory processes involved in nematode infection and allergy.
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      description:"Alternatively-activated" macrophages are found in Th2-mediated inflammatory settings such as nematode infection and allergic pulmonary inflammation. Due in part to a lack of markers, these cells have not been well characterized in vivo and their function remains unknown. We have used murine macrophages elicited by nematode infection (NeMφ) as a source of in vivo derived alternatively activated macrophages. Using three distinct yet complementary molecular approaches we have established a gene expression profile of alternatively activated macrophages and identified macrophage genes that are regulated in vivo by IL-4. First, genes abundantly expressed were identified by an expressed sequence tag strategy. Second, an array of 1176 known mouse genes was screened for differential expression between NeMφ from wild type or IL-4 deficient mice. Third, a subtractive library was screened to identify novel IL-4 dependent macrophage genes. Differential expression was confirmed by real time RT-PCR analysis. Our data demonstrate that alternatively activated macrophages generated in vivo have a gene expression profile distinct from any macrophage population described to date. Several of the genes we identified, including those most abundantly expressed, have not previously been associated with macrophages and thus this study provides unique new information regarding the phenotype of macrophages found in Th2-mediated, chronic inflammatory settings. Our data also provide additional in vivo evidence for parallels between the inflammatory processes involved in nematode infection and allergy.
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