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A high-resolution integrated analysis of genetic and expression profiles of breast cancer cell lines | Breast Cancer Research and Treatment
Description:
Tumour cell lines derived from breast cancer patients constitute one of the cornerstones of breast cancer research. To characterise breast cancer cell lines at the genetic level, we have developed a full tiling path bacterial artificial chromosome (BAC) array collection for comparative genomic hybridisation (aCGH). This aCGH BAC collection covers 98% of the entire human genome at a resolution of 40–60 kbp. We have used this platform alongside an in-house produced 17 K cDNA microarray set to characterise the genetic and transcriptomic profiles of 24 breast cancer cell lines, as well as cell types derived from non-diseased breast. We demonstrate that breast cancer cell lines have genomic and transcriptomic features that recapitulate those of primary breast cancers and can be reliably subclassified into basal-like and luminal subgroups. By overlaying aCGH and transcriptomic data, we have identified 753 genes whose expression correlate with copy number; this list comprised numerous oncogenes recurrently amplified and overexpressed in breast cancer (e.g., HER2, MYC, CCND1 and AURKA). Finally, we demonstrate that although breast cancer cell lines have genomic features usually found in grade III breast cancers (i.e., gains of 1q, 8q and 20q), basal-like and luminal cell lines are characterised by distinct genomic aberrations.
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Keywords {🔍}
cancer, breast, article, google, scholar, pubmed, cas, cell, genomic, res, lines, expression, gene, analysis, research, profiling, author, reisfilho, human, cancers, molecular, amplification, carcinomas, nat, access, clin, genet, profiles, comparative, basallike, privacy, cookies, content, data, mackay, grigoriadis, array, acgh, luminal, genes, cells, lambros, pathol, doipath, esr, common, reply, identification, doican, publish,
Topics {✒️}
reis-filho & alan ashworth month download article/chapter reis-filho js high-resolution genomic profiles 20q13-amplified breast cancers high-resolution integrated analysis full article pdf her2-amplified breast cancers luminal cell lines high-resolution acgh cell types derived anita grigoriadis breast cancer research invasive breast cancer privacy choices/manage cookies human mammary luminal breakthrough breast cancer comparative genomic hybridisation related subjects cancer research uk bmc genomics 7 expression profiling identifies array cgh profiles entire human genome gene-expression subtypes human breast tumours primary breast cancers breast tumor subtypes luminal breast cancers advanced breast cancer breast cancer pathophysiologies primary breast cancer her2/top2a amplicon article mackay false discovery rate gene expression profiling cdna microarray set epigenetically silenced gene metaplastic breast carcinomas lobular breast carcinomas situ hybridization analysis human glioma cells european economic area check access extremely low tumorigenicity transcriptional aberrations linked immunomagnetic separation method large-scale purification pleomorphic lobular carcinoma stat soc [ser
Questions {❓}
- Brenton JD, Carey LA, Ahmed AA et al (2005) Molecular classification and molecular forecasting of breast cancer: ready for clinical application?
- Brown LA, Hoog J, Chin SF et al (2008) ESR1 gene amplification in breast cancer: a common phenomenon?
- Horlings HM, Bergamaschi A, Nordgard SH et al (2008) ESR1 gene amplification in breast cancer: a common phenomenon?
- Reis-Filho JS, Drury S, Lambros MB et al (2008) ESR1 gene amplification in breast cancer: a common phenomenon?
- Vincent-Salomon A, Raynal V, Lucchesi C et al (2008) ESR1 gene amplification in breast cancer: a common phenomenon?
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headline:A high-resolution integrated analysis of genetic and expression profiles of breast cancer cell lines
description:Tumour cell lines derived from breast cancer patients constitute one of the cornerstones of breast cancer research. To characterise breast cancer cell lines at the genetic level, we have developed a full tiling path bacterial artificial chromosome (BAC) array collection for comparative genomic hybridisation (aCGH). This aCGH BAC collection covers 98% of the entire human genome at a resolution of 40–60 kbp. We have used this platform alongside an in-house produced 17 K cDNA microarray set to characterise the genetic and transcriptomic profiles of 24 breast cancer cell lines, as well as cell types derived from non-diseased breast. We demonstrate that breast cancer cell lines have genomic and transcriptomic features that recapitulate those of primary breast cancers and can be reliably subclassified into basal-like and luminal subgroups. By overlaying aCGH and transcriptomic data, we have identified 753 genes whose expression correlate with copy number; this list comprised numerous oncogenes recurrently amplified and overexpressed in breast cancer (e.g., HER2, MYC, CCND1 and AURKA). Finally, we demonstrate that although breast cancer cell lines have genomic features usually found in grade III breast cancers (i.e., gains of 1q, 8q and 20q), basal-like and luminal cell lines are characterised by distinct genomic aberrations.
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