We are analyzing https://link.springer.com/article/10.1007/s12253-018-0453-1.

Title:
Non-Coding RNA Pvt1 Promotes Cancer Stem Cell–Like Traits in Nasopharyngeal Cancer via Inhibiting miR-1207 | Pathology & Oncology Research
Description:
Nasopharyngeal carcinoma (NPC) is a kind of head-neck malignant tumor. lncRNA-PVT1 can promote the proliferation of carcinoma cells, and induce cells to have stem cell-like potentials. However, the function of PVT1 in NPC cells is not clear. The expressions of lncRNA-PVT1 and the expressions of the stem cell markers in NPC tissues or cell lines were investigated by qRT-PCR or western blot. The cell proliferation, and the ability of NPC cells to form spherical, clonal colonies were investigated by MTT assay, colony formation assay, and tumor-sphere formation assay. Cancer stem cells surface markers were detected by flow cytometry and western blot. PI3K/AKT signal activation in NPC cells was determined by western blot. PVT1 was significantly up-regulated in both NPC tissues and cell lines and associated with poor prognosis. PVT1 knockdown reduced NPC cells viability, clonogenicity, the cell surface CD44+/CD24− stem phenotype, and the expressions of the stem cell markers in NPC cells, including Oct4, c-Myc, SOX2, and ALDH. Furthermore, PVT1 negatively regulates the expression levels of miR-1207 in NPC cells and spheres cells, which is critical for NPC stemness. Knockdown of miR-1207 promoted stem phenotype and the expressions of the stem cell markers in NPC cells. Moreover, phosphor-PI3K (p-PI3K) and phosphor-AKT (p-AKT) were found to be down-regulated after PVT1 siRNAs transfection in NPC cells. And miR-1207 inhibitor transfection reversed the all the effects brought by PVT1 knockdown. Pvt1 promotes cancer stem cell–like properties in NPC cells via inhibiting miR-1207 and activating the PI3K/AKT signal pathway.
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Keywords {🔍}

pubmed, google, scholar, cells, cancer, stem, pvt, cas, central, cell, noncoding, rna, long, npc, article, nasopharyngeal, carcinoma, res, promotes, cui, human, research, expression, access, int, zhengzhou, privacy, cookies, content, celllike, chang, wang, tumor, sox, biol, mol, information, publish, search, oncology, proliferation, expressions, markers, prognosis, rnas, clinical, oncol, breast, affiliated, data,

Topics {✒️}

pi3k/akt/mtor signaling pathway month download article/chapter su-xia luo pi3k/akt signal pathway noncoding rna revolution-trashing ji-heng wang tumor-sphere formation assay pi3k/akt signal activation mek/erk pathways involved head-neck malignant tumor twist-positive breast cancer myc copy-number increase recurrent nasopharyngeal carcinoma pvt1 negatively regulates coding rna pvt1 nasopharyngeal carcinoma--review stem cell markers related subjects cancer stem cells hepatocellular carcinoma cells akt/mtor signaling full article pdf privacy choices/manage cookies metastasis stem cells osteosarcoma stem cells long noncoding rnas human gastric cancer hedgehog signaling pathway pvt1 sirnas transfection bladder cancer cells human breast cancer colorectal cancer correlates colony formation assay cerna-cerna network affiliated cancer hospital european economic area jun-fu wu murine pvt-1 locus genomically unstable region genomic context impact human chromosome 8q24 poor clinical outcome article cui noncoding rnas conditions privacy policy carcinoma cells sox2-oct4 connection regulating dna repair regulating apoptotic pathways coding rna

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         description:Nasopharyngeal carcinoma (NPC) is a kind of head-neck malignant tumor. lncRNA-PVT1 can promote the proliferation of carcinoma cells, and induce cells to have stem cell-like potentials. However, the function of PVT1 in NPC cells is not clear. The expressions of lncRNA-PVT1 and the expressions of the stem cell markers in NPC tissues or cell lines were investigated by qRT-PCR or western blot. The cell proliferation, and the ability of NPC cells to form spherical, clonal colonies were investigated by MTT assay, colony formation assay, and tumor-sphere formation assay. Cancer stem cells surface markers were detected by flow cytometry and western blot. PI3K/AKT signal activation in NPC cells was determined by western blot. PVT1 was significantly up-regulated in both NPC tissues and cell lines and associated with poor prognosis. PVT1 knockdown reduced NPC cells viability, clonogenicity, the cell surface CD44+/CD24− stem phenotype, and the expressions of the stem cell markers in NPC cells, including Oct4, c-Myc, SOX2, and ALDH. Furthermore, PVT1 negatively regulates the expression levels of miR-1207 in NPC cells and spheres cells, which is critical for NPC stemness. Knockdown of miR-1207 promoted stem phenotype and the expressions of the stem cell markers in NPC cells. Moreover, phosphor-PI3K (p-PI3K) and phosphor-AKT (p-AKT) were found to be down-regulated after PVT1 siRNAs transfection in NPC cells. And miR-1207 inhibitor transfection reversed the all the effects brought by PVT1 knockdown. Pvt1 promotes cancer stem cell–like properties in NPC cells via inhibiting miR-1207 and activating the PI3K/AKT signal pathway.
         datePublished:2018-08-23T00:00:00Z
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      headline:Non-Coding RNA Pvt1 Promotes Cancer Stem Cell–Like Traits in Nasopharyngeal Cancer via Inhibiting miR-1207
      description:Nasopharyngeal carcinoma (NPC) is a kind of head-neck malignant tumor. lncRNA-PVT1 can promote the proliferation of carcinoma cells, and induce cells to have stem cell-like potentials. However, the function of PVT1 in NPC cells is not clear. The expressions of lncRNA-PVT1 and the expressions of the stem cell markers in NPC tissues or cell lines were investigated by qRT-PCR or western blot. The cell proliferation, and the ability of NPC cells to form spherical, clonal colonies were investigated by MTT assay, colony formation assay, and tumor-sphere formation assay. Cancer stem cells surface markers were detected by flow cytometry and western blot. PI3K/AKT signal activation in NPC cells was determined by western blot. PVT1 was significantly up-regulated in both NPC tissues and cell lines and associated with poor prognosis. PVT1 knockdown reduced NPC cells viability, clonogenicity, the cell surface CD44+/CD24− stem phenotype, and the expressions of the stem cell markers in NPC cells, including Oct4, c-Myc, SOX2, and ALDH. Furthermore, PVT1 negatively regulates the expression levels of miR-1207 in NPC cells and spheres cells, which is critical for NPC stemness. Knockdown of miR-1207 promoted stem phenotype and the expressions of the stem cell markers in NPC cells. Moreover, phosphor-PI3K (p-PI3K) and phosphor-AKT (p-AKT) were found to be down-regulated after PVT1 siRNAs transfection in NPC cells. And miR-1207 inhibitor transfection reversed the all the effects brought by PVT1 knockdown. Pvt1 promotes cancer stem cell–like properties in NPC cells via inhibiting miR-1207 and activating the PI3K/AKT signal pathway.
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         Nasopharyngeal carcinoma
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         Cancer Research
         Oncology
         Pathology
         Immunology
         Biomedicine
         general
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      name:Department of Head & Neck and Thyroid, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China
      name:Department of Head & Neck and Thyroid, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China
      name:Department of Head & Neck and Thyroid, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China
      name:Department of Head & Neck and Thyroid, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China
      name:Department of Head & Neck and Thyroid, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China
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