
NCBI . NLM . NIH . GOV {
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Title[redir]:
MapSplice: Accurate mapping of RNA-seq reads for splice junction discovery - PMC
Description:
The accurate mapping of reads that span splice junctions is a critical component of all analytic techniques that work with RNA-seq data. We introduce a second generation splice detection algorithm, MapSplice, whose focus is high sensitivity and ...
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Keywords {🔍}
splice, alignment, junctions, doi, junction, data, mapsplice, tags, splicing, alternative, alignments, exon, tag, pubmed, google, scholar, specificity, sensitivity, genome, events, set, pmc, sets, spliced, segment, article, tophat, number, segments, rnaseq, transcript, synthetic, anchor, length, figure, coverage, free, quality, sequence, transcripts, search, breast, cancer, cell, exons, skipping, exonic, total, aligned, samples,
Topics {✒️}
fam/mgb-labeled oligonucleotide probes c-terminal proline-rich region paired-end rna-seq data n-terminal ptb domain trans-acting splicing regulators gtpase-activating protein activity previous rna-seq study drug-sensitive fgfr2 mutations rna-seq data sets simulated rna-seq data �single-anchored' alignment starting pmc beta search 10k false-positive junctions long rna-seq reads double-anchored spliced alignments �double-anchored' spliced alignment double-anchored spliced alignment 10k true-positive junctions double-anchor search method mapsplice detected 10k–18k rna-seq protocol samples single-anchored spliced alignment typical h-mer size supplementary data supp_38_18_e178__index hypothetical exon–exon combinations fewer false-positive junctions proline-rich region model single-nucleotide variations distinct splicing event rna-seq data illumina genome analyzer approximate sequence similarity rna-seq reads filtering criteria adopted synthetic data generation license information pmcid nucleic acids res splicing isoform proportions nucleic acids research differential exon usage ptb domain exon cis-acting sequences alternative rna splicing exon skipping events full-length transcripts synthetic data sets rna-seq experiments chimeric splicing events alternative splicing events 10k false junctions
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