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We began analyzing https://www.nature.com/articles/srep09015, but it redirected us to https://www.nature.com/articles/srep09015. The analysis below is for the second page.

Title[redir]:
In vivo CaspaseTracker biosensor system for detecting anastasis and non-apoptotic caspase activity | Scientific Reports
Description:
The discovery that mammalian cells can survive late-stage apoptosis challenges the general assumption that active caspases are markers of impending death. However, tools have not been available to track healthy cells that have experienced caspase activity at any time in the past. Therefore, to determine if cells in whole animals can undergo reversal of apoptosis, known as anastasis, we developed a dual color CaspaseTracker system for Drosophila to identify cells with ongoing or past caspase activity. Transient exposure of healthy females to environmental stresses such as cold shock or starvation activated the CaspaseTracker coincident with caspase activity and apoptotic morphologies in multiple cell types of developing egg chambers. Importantly, when stressed flies were returned to normal conditions, morphologically healthy egg chambers and new progeny flies were labeled by the biosensor, suggesting functional recovery from apoptotic caspase activation. In striking contrast to developing egg chambers, which lack basal caspase biosensor activation under normal conditions, many adult tissues of normal healthy flies exhibit robust caspase biosensor activity in a portion of cells, including neurons. The widespread persistence of CaspaseTracker-positivity implies that healthy cells utilize active caspases for non-apoptotic physiological functions during and after normal development.

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Keywords {🔍}

caspase, cell, article, biosensor, cells, google, scholar, activity, cas, flies, death, drosophila, caspasetracker, apoptosis, caspases, fig, egg, chambers, anastasis, normal, activation, nature, healthy, days, nonapoptotic, nat, system, dqvd, rfp, tang, cold, gal, gfp, biol, apoptotic, functions, development, supplementary, shock, adult, tissues, fly, animals, cancer, protein, dqva, image, newly, eclosed, molecular,

Topics {✒️}

nature portfolio privacy policy author information authors c-terminal 3x-myc tag mcd8-dqvd/a-gal4-myc caspasetracker biosensors advertising nature 471 nature social media tools 0/ reprints cancer research soybean research mcd8-rfp-dqvd-nls-egfp n-end rule pathway caspase-sensitive natural substrate nes-devd-yfp-nls demonstrate caspase-dependent death fluorescence-resonance energy transfer nutri-fly™ bloomington formulation virus-induced neuronal apoptosis drosophila neuron-specific protein drosophila neuron-specific protein44 ho lam tang hong kong aoe/b-07/99 distinguishing long-term recovery full size image bcl-2 protein family apoptotic enteroblast-specific role anti-active caspase immunoreactivity ho man tang ho man tang n-end rule cleaved/active caspase immunoreactivity extensive basal caspasetracker healthy biosensor-positive cells flp recombinase leading gal4 transcription factor middle left panel starvation-induced apoptotic morphologies rare biosensor-positive cells permissions expressed similarly based bcl-2 family proteins basic biological phenomenon indicating caspase-specific cleavage normal day-job functions cleaved-caspase immuno-staining biosensor-positive egg chambers newly eclosed flies

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