Here's how DOI.ORG makes money* and how much!

*Please read our disclaimer before using our estimates.
Loading...

DOI . ORG {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Doi.org Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. Social Networks
  11. External Links
  12. Analytics And Tracking
  13. Libraries
  14. Hosting Providers
  15. CDN Services

We began analyzing https://www.nature.com/articles/s41419-022-04952-0, but it redirected us to https://www.nature.com/articles/s41419-022-04952-0. The analysis below is for the second page.

Title[redir]:
FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2’-deoxycytidine and 5-hydroxymethyl-2’-deoxyuridine | Cell Death & Disease
Description:
Fanconi anemia (FA) is a rare hereditary disorder caused by mutations in any one of the FANC genes. FA cells are mainly characterized by extreme hypersensitivity to interstrand crosslink (ICL) agents. Additionally, the FA proteins play a crucial role in concert with homologous recombination (HR) factors to protect stalled replication forks. Here, we report that the 5-methyl-2’-deoxycytidine (5mdC) demethylation (pathway) intermediate 5-hydroxymethyl-2’-deoxycytidine (5hmdC) and its deamination product 5-hydroxymethyl-2’-deoxyuridine (5hmdU) elicit a DNA damage response, chromosome aberrations, replication fork impairment and cell viability loss in the absence of FANCD2. Interestingly, replication fork instability by 5hmdC or 5hmdU was associated to the presence of Poly(ADP-ribose) polymerase 1 (PARP1) on chromatin, being both phenotypes exacerbated by olaparib treatment. Remarkably, Parp1−/− cells did not show any replication fork defects or sensitivity to 5hmdC or 5hmdU, suggesting that retained PARP1 at base excision repair (BER) intermediates accounts for the observed replication fork defects upon 5hmdC or 5hmdU incorporation in the absence of FANCD2. We therefore conclude that 5hmdC is deaminated in vivo to 5hmdU, whose fixation by PARP1 during BER, hinders replication fork progression and contributes to genomic instability in FA cells.

Matching Content Categories {📚}

  • Science
  • Telecommunications
  • Education

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,420 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Doi.org Make Money? {💸}

We don't see any clear sign of profit-making.

Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Doi.org could be getting rich in stealth mode, or the way it's monetizing isn't detectable.

Keywords {🔍}

hmdc, cells, fancd, dna, replication, pubmed, hmdu, parp, cell, fork, article, fig, wild, type, google, scholar, cas, central, olaparib, min, exposure, supplementary, exposed, test, nature, pathway, plot, genomic, represents, damage, repair, dose, line, suggesting, γhax, increased, hmdcd, instability, kbp, mefs, left, forks, absence, incorporation, data, defects, progression, bases, mdc, chromatin,

Topics {✒️}

figure legends nature portfolio integrated hplc/esi-ms/ms system privacy policy supplementary information files advertising crispr/cas9-mediated gene disruptions ung-backup uracil-excision activities thermo scientific 5hmdc-induced γ-h2ax foci universidad de sevilla maría josé peña-gómez supplementary fig reprints goat anti-rat alexa-488 nature nature 1993 nuclear γ-h2ax foci hplc-ms/ms quantitation fancd2− crispr-cas9 knocked article peña-gómez streptavidin-magnetic sepharose beads van rossum-fikkert se γ-h2ax foci formation mouse anti-brdu antibody replication fork-structured dna prevents double-strand breaks accumulate single-stranded dna rat anti-brdu antibody functional fa/brca pathway deamination product 5-hydroxymethyl-2’-deoxyuridine social media fresh media cell line-dependent manner dna repair-deficient fancc hplc-ms/ms cytosine-5-methyltransferases add aldehydes central line represents 5hmdc-mediated fork impairment olaparib dose-dependent death transcription-replication conflicts permissions 5hmdc-mediated dna damage dot plot representing trapped parp1-replication conflicts replication fork stability 5hmdc-derived 5hmdu accumulates fbh1-dependent rad51 regulation regulates gene transcription cell death dis

Questions {❓}

  • Why does the bone marrow fail in Fanconi anemia?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2’-deoxycytidine and 5-hydroxymethyl-2’-deoxyuridine
         description:Fanconi anemia (FA) is a rare hereditary disorder caused by mutations in any one of the FANC genes. FA cells are mainly characterized by extreme hypersensitivity to interstrand crosslink (ICL) agents. Additionally, the FA proteins play a crucial role in concert with homologous recombination (HR) factors to protect stalled replication forks. Here, we report that the 5-methyl-2’-deoxycytidine (5mdC) demethylation (pathway) intermediate 5-hydroxymethyl-2’-deoxycytidine (5hmdC) and its deamination product 5-hydroxymethyl-2’-deoxyuridine (5hmdU) elicit a DNA damage response, chromosome aberrations, replication fork impairment and cell viability loss in the absence of FANCD2. Interestingly, replication fork instability by 5hmdC or 5hmdU was associated to the presence of Poly(ADP-ribose) polymerase 1 (PARP1) on chromatin, being both phenotypes exacerbated by olaparib treatment. Remarkably, Parp1−/− cells did not show any replication fork defects or sensitivity to 5hmdC or 5hmdU, suggesting that retained PARP1 at base excision repair (BER) intermediates accounts for the observed replication fork defects upon 5hmdC or 5hmdU incorporation in the absence of FANCD2. We therefore conclude that 5hmdC is deaminated in vivo to 5hmdU, whose fixation by PARP1 during BER, hinders replication fork progression and contributes to genomic instability in FA cells.
         datePublished:2022-05-27T00:00:00Z
         dateModified:2022-05-27T00:00:00Z
         pageStart:1
         pageEnd:11
         license:http://creativecommons.org/licenses/by/4.0/
         sameAs:https://doi.org/10.1038/s41419-022-04952-0
         keywords:
            DNA damage and repair
            DNA replication
            Life Sciences
            general
            Biochemistry
            Cell Biology
            Immunology
            Cell Culture
            Antibodies
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig4_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig5_HTML.png
         isPartOf:
            name:Cell Death & Disease
            issn:
               2041-4889
            volumeNumber:13
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Nature Publishing Group UK
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:María José Peña-Gómez
               affiliation:
                     name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
                     address:
                        name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Paula Moreno-Gordillo
               affiliation:
                     name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
                     address:
                        name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Milda Narmontė
               url:http://orcid.org/0000-0001-8568-0516
               affiliation:
                     name:Vilnius University
                     address:
                        name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Clara B. García-Calderón
               affiliation:
                     name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío
                     address:
                        name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío, Seville, Spain
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Audronė Rukšėnaitė
               affiliation:
                     name:Vilnius University
                     address:
                        name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Saulius KlimaÅ¡auskas
               url:http://orcid.org/0000-0002-1395-2030
               affiliation:
                     name:Vilnius University
                     address:
                        name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Iván V. Rosado
               url:http://orcid.org/0000-0001-7409-7341
               affiliation:
                     name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
                     address:
                        name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
                        type:PostalAddress
                     type:Organization
                     name:Universidad de Sevilla
                     address:
                        name:Departamento de Genética, Universidad de Sevilla, Seville, Spain
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:1
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2’-deoxycytidine and 5-hydroxymethyl-2’-deoxyuridine
      description:Fanconi anemia (FA) is a rare hereditary disorder caused by mutations in any one of the FANC genes. FA cells are mainly characterized by extreme hypersensitivity to interstrand crosslink (ICL) agents. Additionally, the FA proteins play a crucial role in concert with homologous recombination (HR) factors to protect stalled replication forks. Here, we report that the 5-methyl-2’-deoxycytidine (5mdC) demethylation (pathway) intermediate 5-hydroxymethyl-2’-deoxycytidine (5hmdC) and its deamination product 5-hydroxymethyl-2’-deoxyuridine (5hmdU) elicit a DNA damage response, chromosome aberrations, replication fork impairment and cell viability loss in the absence of FANCD2. Interestingly, replication fork instability by 5hmdC or 5hmdU was associated to the presence of Poly(ADP-ribose) polymerase 1 (PARP1) on chromatin, being both phenotypes exacerbated by olaparib treatment. Remarkably, Parp1−/− cells did not show any replication fork defects or sensitivity to 5hmdC or 5hmdU, suggesting that retained PARP1 at base excision repair (BER) intermediates accounts for the observed replication fork defects upon 5hmdC or 5hmdU incorporation in the absence of FANCD2. We therefore conclude that 5hmdC is deaminated in vivo to 5hmdU, whose fixation by PARP1 during BER, hinders replication fork progression and contributes to genomic instability in FA cells.
      datePublished:2022-05-27T00:00:00Z
      dateModified:2022-05-27T00:00:00Z
      pageStart:1
      pageEnd:11
      license:http://creativecommons.org/licenses/by/4.0/
      sameAs:https://doi.org/10.1038/s41419-022-04952-0
      keywords:
         DNA damage and repair
         DNA replication
         Life Sciences
         general
         Biochemistry
         Cell Biology
         Immunology
         Cell Culture
         Antibodies
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig4_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1038%2Fs41419-022-04952-0/MediaObjects/41419_2022_4952_Fig5_HTML.png
      isPartOf:
         name:Cell Death & Disease
         issn:
            2041-4889
         volumeNumber:13
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Nature Publishing Group UK
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:María José Peña-Gómez
            affiliation:
                  name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
                  address:
                     name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Paula Moreno-Gordillo
            affiliation:
                  name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
                  address:
                     name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Milda Narmontė
            url:http://orcid.org/0000-0001-8568-0516
            affiliation:
                  name:Vilnius University
                  address:
                     name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Clara B. García-Calderón
            affiliation:
                  name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío
                  address:
                     name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío, Seville, Spain
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Audronė Rukšėnaitė
            affiliation:
                  name:Vilnius University
                  address:
                     name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Saulius KlimaÅ¡auskas
            url:http://orcid.org/0000-0002-1395-2030
            affiliation:
                  name:Vilnius University
                  address:
                     name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Iván V. Rosado
            url:http://orcid.org/0000-0001-7409-7341
            affiliation:
                  name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
                  address:
                     name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
                     type:PostalAddress
                  type:Organization
                  name:Universidad de Sevilla
                  address:
                     name:Departamento de Genética, Universidad de Sevilla, Seville, Spain
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:1
["Periodical","PublicationVolume"]:
      name:Cell Death & Disease
      issn:
         2041-4889
      volumeNumber:13
Organization:
      name:Nature Publishing Group UK
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
      address:
         name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
         type:PostalAddress
      name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
      address:
         name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
         type:PostalAddress
      name:Vilnius University
      address:
         name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
         type:PostalAddress
      name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío
      address:
         name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío, Seville, Spain
         type:PostalAddress
      name:Vilnius University
      address:
         name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
         type:PostalAddress
      name:Vilnius University
      address:
         name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
         type:PostalAddress
      name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
      address:
         name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
         type:PostalAddress
      name:Universidad de Sevilla
      address:
         name:Departamento de Genética, Universidad de Sevilla, Seville, Spain
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:María José Peña-Gómez
      affiliation:
            name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
            address:
               name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
               type:PostalAddress
            type:Organization
      name:Paula Moreno-Gordillo
      affiliation:
            name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
            address:
               name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
               type:PostalAddress
            type:Organization
      name:Milda Narmontė
      url:http://orcid.org/0000-0001-8568-0516
      affiliation:
            name:Vilnius University
            address:
               name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
               type:PostalAddress
            type:Organization
      name:Clara B. García-Calderón
      affiliation:
            name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío
            address:
               name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío, Seville, Spain
               type:PostalAddress
            type:Organization
      name:Audronė Rukšėnaitė
      affiliation:
            name:Vilnius University
            address:
               name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
               type:PostalAddress
            type:Organization
      name:Saulius KlimaÅ¡auskas
      url:http://orcid.org/0000-0002-1395-2030
      affiliation:
            name:Vilnius University
            address:
               name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
               type:PostalAddress
            type:Organization
      name:Iván V. Rosado
      url:http://orcid.org/0000-0001-7409-7341
      affiliation:
            name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide
            address:
               name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
               type:PostalAddress
            type:Organization
            name:Universidad de Sevilla
            address:
               name:Departamento de Genética, Universidad de Sevilla, Seville, Spain
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
      name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
      name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
      name:Instituto de Biomedicina de Sevilla (IBiS)/CSIC/Universidad de Sevilla/Campus Hospital Universitario Virgen del Rocío, Seville, Spain
      name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
      name:Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius, Lithuania
      name:Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER) Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain
      name:Departamento de Genética, Universidad de Sevilla, Seville, Spain

Social Networks {👍}(1)

External Links {🔗}(383)

Analytics and Tracking {📊}

  • Google Tag Manager

Libraries {📚}

  • Prism.js
  • Zoom.js

Emails and Hosting {✉️}

Mail Servers:

  • mx.zoho.eu
  • mx2.zoho.eu
  • mx3.zoho.eu

Name Servers:

  • josh.ns.cloudflare.com
  • zita.ns.cloudflare.com

CDN Services {📦}

  • Crossref

5.49s.