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  1. Analyzed Page
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We began analyzing https://www.nature.com/articles/s41366-018-0276-x, but it redirected us to https://www.nature.com/articles/s41366-018-0276-x. The analysis below is for the second page.

Title[redir]:
Dual targeting of Nur77 and AMPKα by isoalantolactone inhibits adipogenesis in vitro and decreases body fat mass in vivo | International Journal of Obesity
Description:
Suppression of adipogenesis has been considered as a potential target for the prevention and treatment of obesity and associated metabolic disorders, and the nuclear receptor 4A1 (NR4A1/Nur77) and AMPKα are known to play important roles during early and intermediate stages of adipogenesis. Therefore, we hypothesized that dual targeting Nur77 and AMPKα would show strong inhibitory effect on adipogenesis. We screened a herbal medicine-based small molecule library to identify novel natural compounds dual targeting Nur77 and AMPKα, and the antiadipogenic effects and mechanisms of action of a “hit” compound were studied in 3T3-L1 cells. In vivo antiobesity effects of the compound were also investigated in high-fat diet (HFD)-induced obese mice. We identified isoalantolactone (ISO) as a new NR4A1 inactivator that also activates AMPKα in 3T3-L1 cells. ISO, as expected, inhibited adipogenic differentiation of 3T3-L1 preadipocytes, accompanied by reduced mitotic clonal expansion (MCE) which occurs in the early stage of adipogenesis and decreased expression of genes required for MCE and cell cycle markers including cyclin A, cyclin D1. Furthermore, ISO reduced body weight gain and fat mass (epididymal, subcutaneous, perirenal, and inguinal white adipose tissues) in the high-fat diet-fed C57BL/6 N mice. Serum levels of triglycerides, aspartate transaminase, and alanine transaminase and hepatic steatosis were also significantly improved in the ISO-treated group compared to the high-fat diet control group. These results suggest that ISO dual targeting Nur77 and AMPKα during adipogenesis represents a novel class of mechanism-based antiadipogenic agents for treatment of obesity and associated metabolic disorders, including hyperlipidemia and fatty liver.

Matching Content Categories {📚}

  • Science
  • Education
  • Health & Fitness

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

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Traffic Estimate {📈}

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🏙️ Massive Traffic: 50M - 100M visitors per month


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Keywords {🔍}

article, google, scholar, cas, nature, adipogenesis, lee, obesity, nuclear, access, receptor, mice, adipose, content, ampkα, kim, cells, ampk, tissue, cancer, development, cookies, data, targeting, inhibits, fat, safe, chen, research, korea, privacy, journal, cell, dual, body, metabolic, highfat, preadipocytes, metab, endocrinol, orphan, physiol, republic, information, adipocyte, effects, diet, iso, differentiation, mitotic,

Topics {✒️}

nature portfolio permissions reprints privacy policy cooperative research program nature ccaat/enhancer-binding protein beta advertising portal theory social media high-fat diet-fed c57bl/6 hye-rin cho syng-ook lee amp-activated protein kinase mechanism-based antiadipogenic agents author information authors iso-treated group compared keuk-jun kim joung-hee kim vivo yeon-seop jung insulin-resistant black men orphan receptor tr3 3t3-l1 preadipocytes depends springerlink instant access rural development administration orphan nuclear receptors author correspondence permissions technology development [project adipocyte development nuclear receptor 4a1 nuclear receptor atlas white adipose tissue physiol cell physiol dual targeting nur77 high-fat diet endocr relat cancer personal data mitotic clonal expansion privacy nat cell biol arctiin inhibits adipogenesis pancreatic cancer cells visceral adipose tissue isoalantolactone inhibits adipogenesis 3t3-l1 cells proc nutr soc breast cancer chemotherapy data protection black adzuki bean explore content

Schema {🗺️}

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         description:Suppression of adipogenesis has been considered as a potential target for the prevention and treatment of obesity and associated metabolic disorders, and the nuclear receptor 4A1 (NR4A1/Nur77) and AMPKα are known to play important roles during early and intermediate stages of adipogenesis. Therefore, we hypothesized that dual targeting Nur77 and AMPKα would show strong inhibitory effect on adipogenesis. We screened a herbal medicine-based small molecule library to identify novel natural compounds dual targeting Nur77 and AMPKα, and the antiadipogenic effects and mechanisms of action of a “hit” compound were studied in 3T3-L1 cells. In vivo antiobesity effects of the compound were also investigated in high-fat diet (HFD)-induced obese mice. We identified isoalantolactone (ISO) as a new NR4A1 inactivator that also activates AMPKα in 3T3-L1 cells. ISO, as expected, inhibited adipogenic differentiation of 3T3-L1 preadipocytes, accompanied by reduced mitotic clonal expansion (MCE) which occurs in the early stage of adipogenesis and decreased expression of genes required for MCE and cell cycle markers including cyclin A, cyclin D1. Furthermore, ISO reduced body weight gain and fat mass (epididymal, subcutaneous, perirenal, and inguinal white adipose tissues) in the high-fat diet-fed C57BL/6 N mice. Serum levels of triglycerides, aspartate transaminase, and alanine transaminase and hepatic steatosis were also significantly improved in the ISO-treated group compared to the high-fat diet control group. These results suggest that ISO dual targeting Nur77 and AMPKα during adipogenesis represents a novel class of mechanism-based antiadipogenic agents for treatment of obesity and associated metabolic disorders, including hyperlipidemia and fatty liver.
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Social Networks {👍}(1)

External Links {🔗}(183)

Analytics and Tracking {📊}

  • Google Tag Manager

Libraries {📚}

  • Prism.js
  • Zoom.js

Emails and Hosting {✉️}

Mail Servers:

  • mx.zoho.eu
  • mx2.zoho.eu
  • mx3.zoho.eu

Name Servers:

  • josh.ns.cloudflare.com
  • zita.ns.cloudflare.com
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