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We began analyzing https://www.nature.com/articles/nature02516, but it redirected us to https://www.nature.com/articles/nature02516. The analysis below is for the second page.

Title[redir]:
Regulation of ethylene gas biosynthesis by the Arabidopsis ETO1 protein | Nature
Description:
Ethylene gas is used as a hormone by plants, in which it acts as a critical growth regulator. Its synthesis is also rapidly evoked in response to a variety of biotic and abiotic stresses1,2. The Arabidopsis ethylene-overproducer mutants eto2 and eto3 have previously been identified as having mutations in two genes, ACS5 and ACS9, respectively; these encode isozymes of 1-aminocyclopropane-1-carboxylic acid synthase (ACS), which catalyse the rate-limiting step in ethylene biosynthesis3,4. Here we report that another ethylene-overproducer mutation, eto1, is in a gene that negatively regulates ACS activity and ethylene production. The ETO1 protein directly interacts with and inhibits the enzyme activity of full-length ACS5 but not of a truncated form of the enzyme, resulting in a marked accumulation of ACS5 protein and ethylene. Overexpression of ETO1 inhibited induction of ethylene production by the plant growth regulator cytokinin, and promoted ACS5 degradation by a proteasome-dependent pathway. ETO1 also interacts with CUL3, a constituent of ubiquitin ligase complexes in which we propose that ETO1 serves as a substrate-specific adaptor protein. ETO1 thus has a dual mechanism, inhibiting ACS enzyme activity and targeting it for protein degradation. This permits rapid modulation of the concentration of ethylene.

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Keywords {🔍}

article, cas, google, scholar, ethylene, nature, plant, arabidopsis, eto, protein, cell, biosynthesis, access, acs, biol, ecker, synthase, ads, research, content, response, cookies, regulation, wang, yoshida, lurin, enzyme, aminocyclopropanecarboxylate, science, privacy, analysis, data, information, gas, hitoshi, claire, gene, pathway, ubiquitin, family, open, mol, usa, kevin, joseph, plants, acid, activity, cul, support,

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nature portfolio permissions reprints privacy policy advertising nature cell biol social media subscribe nature nature 425 nature 402 nature 428 nature rice research 1-aminocyclopropane-1-carboxylate synthase reveals 1-aminocyclopropane-1-carboxylic acid synthase plant development author correspondence identify ethylene-related mutants univector plasmid-fusion system research scfebf1/ebf2-dependent proteolysis springerlink instant access substrate-specific adaptor protein btb/poz domain proteins permissions supplementary data personal data 1-aminocyclopropane-1-carboxylate synthase stress response pathway 1-aminocyclopropane-1-carboxylic acid ethylene gas biosynthesis data protection ethylene-overproducer mutation privacy substrate-specific adaptor shared active sites competing financial interests plant hormone ethylene ethylene precursor acc ethylene signal transduction substrate-specific adaptors ethylene biosynthesis pathway ethylene response pathway explore content subscription content proteasome-dependent pathway arabidopsis gene family putative substrate adaptors ubiquitin ligase complexes modular ubiquitin ligase ubiquitin–proteasome pathway

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