
DOI . ORG {
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Title[redir]:
Engineering humanized mice for improved hematopoietic reconstitution | Cellular & Molecular Immunology
Description:
Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.
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pubmed, google, scholar, cas, human, cells, blood, mice, central, stem, cell, hematopoietic, gene, humanized, nature, mouse, development, cord, article, chen, research, expansion, vivo, engraftment, science, ther, access, immunol, expression, usa, therapy, lentiviral, model, immune, nodscid, content, reconstitution, shultz, proc, natl, acad, sci, nat, regulatory, virus, med, transfer, adenoassociated, vectors, mol,
Topics {✒️}
nature portfolio permissions reprints privacy policy nod/ltsz-scid il2rγnull mice granulocyte–macrophage colony-stimulating factor long-term culture-initiating cells nature 2006 nature advertising human il-3/gm-csf knock t-cell-dependent antibody responses social media translational biomedical research specific research applications nod-scid il2rγnull mice long-term cytokine production nod/scid-repopulating cells antigen-presenting cells derived humanized rag2−/−γc−/− mice hla-restricted immune responses integrative cancer research multipotent hematopoietic progenitors immunocompetent scid-hu mice human t-cell reconstitution dna methylation-mediated silencing multiple ets factors nod/scid/γcnull mouse cd34+ac133+ cells isolated patient-derived xenografts eugenia implantable pre-metastatic niches lentivirus-mediated gene therapy human cd4+foxp3+ regulatory cell type-specific manner long-term multilineage engraftment hematopoietic progenitor cells wiskott–aldrich syndrome gene murine bernard-soulier syndrome immunodeficient mouse model—rag2 virus-mediated gene transfer human hematopoietic stem human hematopoietic cells personal data hematopoietic stem cells anti-cd47 antibody synergizes springerlink instant access umbilical cord blood human immune system nod/scid/γcnull nod-scid mouse human immune responses
Questions {❓}
- Gene therapy: is IL2RG oncogenic in T-cell development?
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WebPage:
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headline:Engineering humanized mice for improved hematopoietic reconstitution
description:Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.
datePublished:2012-03-19T00:00:00Z
dateModified:2012-03-19T00:00:00Z
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expansion
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humanized mice
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Medical Microbiology
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headline:Engineering humanized mice for improved hematopoietic reconstitution
description:Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.
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pageStart:215
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cytokines
expansion
genetic modification
hematopoietic stem cells
humanized mice
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