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Title[redir]:
Estrogen-Related Receptor Alpha (ERRα) Promotes Cancer Stem Cell-Like Characteristics in Breast Cancer | Stem Cell Reviews and Reports
Description:
Cancer stem cells drive tumor initiation, invasion, metastasis and recurrence. In the present study, we have evaluated the role of ERRα in the maintenance of breast cancer stem cells (BCSCs) using breast cancer cell lines. The inhibition of ERRα with the inverse agonist, XCT-790, or the knockdown of ERRα in breast cancer cells significantly reduced the mammosphere formation efficiency and mammosphere size along with a significant reduction in the CD44+/CD24ā BCSCs. Treatment with XCT-790 significantly downregulated expression of the transcription factors involved in stem cell maintenance such as Oct4, Klf4, Sox2, Nanog and c-Myc in the mammosphere forming stem cells of MCF7 and MDA-MB-231. In addition, XCT-790 induced cell cycle arrest and apoptosis in the mammosphere-forming cells. The knockdown or inhibition of ERRα downregulated the expression of Notch1 and β-catenin, whereas the overexpression of ERRα in MCF7 cells upregulated the expression of these proteins. Moreover, the inhibition of ERRα synergistically enhanced the efficacy of paclitaxel in inhibiting the BCSCs. These results show that ERRα is crucial for the maintenance of BCSCs and suggest that ERRα could be a potential target for breast cancer treatment. Graphical Abstract
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Keywords {š}
cancer, pubmed, article, breast, google, scholar, stem, cells, cas, cell, central, research, errα, receptor, estrogenrelated, alpha, growth, factor, journal, data, nature, therapy, author, clinical, privacy, cookies, content, information, reviews, xct, mammosphere, expression, notch, target, access, applicable, human, molecular, httpsdoiorgcan, authors, springer, publish, search, manuscript, bcscs, oct, targeting, stemlike, biology, cellular,
Topics {āļø}
estrogen-related receptor alpha egfr-creb/grβ-il-6 axis notch1-induced mammary tumorigenesis er-nfĸb-driven stem month download article/chapter stem/progenitor cell properties triple-negative breast cancer her2-negative breast cancer cancer stem cells b-cell lymphoma predicted differential β-catenin expression breast cancer stem estrogen-related receptors stem cells transform muc1-positive cervical cancer mammosphere-forming cells stem cell properties ovarian cancer cells stem cell rev human breast carcinoma nature reviews cancer stem cell maintenance related subjects full article pdf breast cancer research stem cells code availability oestrogen-related receptors breast cancer published breast cancer treatment privacy choices/manage cookies mcf7 cells upregulated mammosphere formation efficiency flow cytometry data nuclear factor kappa potent prognostic factor selvakumar elangovan cancer cells clinical cancer research detecting/monitoring cancer transcription factors involved β-catenin article muduli breast cancer cancer cell her2/igf-1r holds exclusive rights engineering research board wnt pathways european economic area
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headline:Estrogen-Related Receptor Alpha (ERRα) Promotes Cancer Stem Cell-Like Characteristics in Breast Cancer
description:Cancer stem cells drive tumor initiation, invasion, metastasis and recurrence. In the present study, we have evaluated the role of ERRα in the maintenance of breast cancer stem cells (BCSCs) using breast cancer cell lines. The inhibition of ERRα with the inverse agonist, XCT-790, or the knockdown of ERRα in breast cancer cells significantly reduced the mammosphere formation efficiency and mammosphere size along with a significant reduction in the CD44+/CD24ā BCSCs. Treatment with XCT-790 significantly downregulated expression of the transcription factors involved in stem cell maintenance such as Oct4, Klf4, Sox2, Nanog and c-Myc in the mammosphere forming stem cells of MCF7 and MDA-MB-231. In addition, XCT-790 induced cell cycle arrest and apoptosis in the mammosphere-forming cells. The knockdown or inhibition of ERRα downregulated the expression of Notch1 and β-catenin, whereas the overexpression of ERRα in MCF7 cells upregulated the expression of these proteins. Moreover, the inhibition of ERRα synergistically enhanced the efficacy of paclitaxel in inhibiting the BCSCs. These results show that ERRα is crucial for the maintenance of BCSCs and suggest that ERRα could be a potential target for breast cancer treatment.
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description:Cancer stem cells drive tumor initiation, invasion, metastasis and recurrence. In the present study, we have evaluated the role of ERRα in the maintenance of breast cancer stem cells (BCSCs) using breast cancer cell lines. The inhibition of ERRα with the inverse agonist, XCT-790, or the knockdown of ERRα in breast cancer cells significantly reduced the mammosphere formation efficiency and mammosphere size along with a significant reduction in the CD44+/CD24ā BCSCs. Treatment with XCT-790 significantly downregulated expression of the transcription factors involved in stem cell maintenance such as Oct4, Klf4, Sox2, Nanog and c-Myc in the mammosphere forming stem cells of MCF7 and MDA-MB-231. In addition, XCT-790 induced cell cycle arrest and apoptosis in the mammosphere-forming cells. The knockdown or inhibition of ERRα downregulated the expression of Notch1 and β-catenin, whereas the overexpression of ERRα in MCF7 cells upregulated the expression of these proteins. Moreover, the inhibition of ERRα synergistically enhanced the efficacy of paclitaxel in inhibiting the BCSCs. These results show that ERRα is crucial for the maintenance of BCSCs and suggest that ERRα could be a potential target for breast cancer treatment.
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