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Title[redir]:
Hormone-Responsive Model of Primary Human Breast Epithelium | Journal of Mammary Gland Biology and Neoplasia
Description:
Retention of hormone responsiveness in primary culture models of human breast is essential for studies aimed at understanding the mechanisms of action of the ovarian hormones in the human breast. In this chapter we describe the development of a culture model of primary human breast that retains critical features of the tissue in vivo. We find that primary normal human breast tissue in embedded culture recapitulates the morphology, cell lineages, functional gene expression characteristics and estrogen and progesterone receptor responsiveness of the breast in vivo. The ratio of luminal to myoepithelial cells after culture recapitulates that observed in the uncultured tissue, highlighting the fact that progenitor cells capable of giving rise to both epithelial cell lineages are retained in this model system. By contrast, primary cells placed into monolayer culture, even for a single passage, lose bipotent progenitors, and the myoepithelial lineage predominates, demonstrating the rapidity with which phenotypic changes and selection occur in normal breast cells, unless cultured under conditions that prevent this outcome. Primary matrix-embedded culture of normal human breast cells provides researchers with a new opportunity to understand ovarian hormone action in the human breast.
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Keywords {š}
breast, article, google, scholar, human, cas, pubmed, cells, culture, normal, cancer, mammary, clarke, progesterone, receptor, primary, mote, research, graham, salagame, tissue, cell, estrogen, privacy, cookies, content, journal, gland, neoplasia, model, rosemary, balleine, christine, models, development, access, westmead, south, wales, australia, data, information, publish, search, biology, november, dinny, patricia, usha, myoepithelial,
Topics {āļø}
month download article/chapter mammary myoepithelial cellācinderella primary culture models dimensional culture models primary matrix-embedded culture ļæ½knockinā mouse models stem cells hormone-responsive model mammary gland biology normal breast cells full article pdf embedded culture recapitulates epithelial cell lineages privacy choices/manage cookies normal human breast human mammary development progesterone receptor āknockoutā culture model animal tissue techniques primary human breast phosphate-buffered saline breast cancer res progesterone receptor responsiveness semin cancer biol medical research council progenitor cells capable european economic area basement membrane deposition normal breast development medical research institute human breast tissues myoepithelial lineage predominates human breast development breast cancer risk scope submit manuscript lose bipotent progenitors related subjects modeling dynamic reciprocity dna replication licensing cutting paraffin sections retains critical features performing pas staining primary cells accepting optional cookies culture recapitulates cancer research myoepithelial cells breast tissue article journal journal finder publish
Questions {ā}
- Estrogen receptor null mice: what have we learned and where will they lead us?
- The mammary myoepithelial cellāCinderella or ugly sister?
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headline:Hormone-Responsive Model of Primary Human Breast Epithelium
description:Retention of hormone responsiveness in primary culture models of human breast is essential for studies aimed at understanding the mechanisms of action of the ovarian hormones in the human breast. In this chapter we describe the development of a culture model of primary human breast that retains critical features of the tissue in vivo. We find that primary normal human breast tissue in embedded culture recapitulates the morphology, cell lineages, functional gene expression characteristics and estrogen and progesterone receptor responsiveness of the breast in vivo. The ratio of luminal to myoepithelial cells after culture recapitulates that observed in the uncultured tissue, highlighting the fact that progenitor cells capable of giving rise to both epithelial cell lineages are retained in this model system. By contrast, primary cells placed into monolayer culture, even for a single passage, lose bipotent progenitors, and the myoepithelial lineage predominates, demonstrating the rapidity with which phenotypic changes and selection occur in normal breast cells, unless cultured under conditions that prevent this outcome. Primary matrix-embedded culture of normal human breast cells provides researchers with a new opportunity to understand ovarian hormone action in the human breast.
datePublished:2009-11-21T00:00:00Z
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Progesterone receptor
Normal breast
Proliferation
Oncology
Cancer Research
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headline:Hormone-Responsive Model of Primary Human Breast Epithelium
description:Retention of hormone responsiveness in primary culture models of human breast is essential for studies aimed at understanding the mechanisms of action of the ovarian hormones in the human breast. In this chapter we describe the development of a culture model of primary human breast that retains critical features of the tissue in vivo. We find that primary normal human breast tissue in embedded culture recapitulates the morphology, cell lineages, functional gene expression characteristics and estrogen and progesterone receptor responsiveness of the breast in vivo. The ratio of luminal to myoepithelial cells after culture recapitulates that observed in the uncultured tissue, highlighting the fact that progenitor cells capable of giving rise to both epithelial cell lineages are retained in this model system. By contrast, primary cells placed into monolayer culture, even for a single passage, lose bipotent progenitors, and the myoepithelial lineage predominates, demonstrating the rapidity with which phenotypic changes and selection occur in normal breast cells, unless cultured under conditions that prevent this outcome. Primary matrix-embedded culture of normal human breast cells provides researchers with a new opportunity to understand ovarian hormone action in the human breast.
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Normal breast
Proliferation
Oncology
Cancer Research
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