DOI . ORG {}

Analyzed Page
Matching Content Categories
CMS
Monthly Traffic Estimate
How Does Doi.org Make Money
Keywords
Topics
Schema
External Links
Analytics And Tracking
Libraries
Hosting Providers
CDN Services

We began analyzing https://link.springer.com/article/10.1007/BF02623435, but it redirected us to https://link.springer.com/article/10.1007/BF02623435. The analysis below is for the second page.

Title[redir]:
A simplified method for passage and long-term growth of human mammary epithelial cells | In Vitro Cellular & Developmental Biology - Plant
Description:
A method is described for culturing human mammary epithelial cells in primary culture and allowing more than 50 generations and a 1000-fold increase from starting inocula without need of enzymatic transfers. Organoids dissociated from breast tissue are plated in medium containing 1.05 mM Ca++ to effect attachment and growth to monolayer density. Medium is then switched to one containing 0.06 mM Ca++ to overcome “renewal inhibition” and to stimulate growth. In low Ca++ media, primary cultures become a long-term, continuous source of free-floating viable cells free of fibroblasts. A fundamental requirement for extended growth in primary culture is maintaining calcium levels at approximately 0.06 mM. Above 0.06 mM Ca++, cells divide only 3 to 4 times in primary cultures before terminal differentiation occurs. At 0.06 mM Ca++, cells continue to divide for periods of time determined partly by feeding schedule, but up to 6 mo. and 50 generations of (linear) growth. Cells released from monolayer were greater than 90% viable and yielded 105 cells/cm2 of attached cells every 72 h. Free-floating single cells readily replated and cloned, when transferred, without need of trypsin for dissociation. Long-term free-floating cells were typical mammary epithelium: (a) they formed domes and exhibited renewal inhibition, (b) they produced ductlike formations in collagen gels, (c) they contained epithelium-specific keratin filaments, and (d) they were diploid.

Matching Content Categories {📚}

Science
Education
Careers

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Doi.org Make Money? {💸}

We find it hard to spot revenue streams.

The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Doi.org might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

cells, article, growth, mammary, google, scholar, culture, cell, human, pubmed, cas, vitro, epithelial, soule, medium, calcium, privacy, cookies, content, mcgrath, primary, publish, search, method, longterm, access, stampfer, normal, cancer, data, information, log, journal, research, cellular, biology, passage, tissue, monolayer, renewal, inhibition, cultures, freefloating, epithelium, chapter, discover, proliferation, proc, natl, usa,

Topics {✒️}

month download article/chapter long-term free-floating cells human cell line tissue culture medium human mammary epithelium cell culture related subjects typical mammary epithelium mouse mammary epithelium privacy choices/manage cookies full article pdf tumor-specific properties yielded 105 cells/cm2 serum-free growth maintaining calcium levels cell cycle cell physiol long-term growth european economic area time determined partly produced ductlike formations government printing office epithelial cells pleural effusion derived cholera toxin stimulation conditions privacy policy breast tissue rapid clonal growth overcome “renewal inhibition” exhibited renewal inhibition accepting optional cookies terminal differentiation occurs lymphoblast proliferation mcgrath rights michigan cancer foundation low ca++ media tumor biology primary culture main content log hormonally defined medium monolayer cultures suitable journal finder publish extended serial passage article soule organoids dissociated culture medium article log check access instant access cells continue

Schema {🗺️}

WebPage:
      mainEntity:
         headline:A simplified method for passage and long-term growth of human mammary epithelial cells
         description:A method is described for culturing human mammary epithelial cells in primary culture and allowing more than 50 generations and a 1000-fold increase from starting inocula without need of enzymatic transfers. Organoids dissociated from breast tissue are plated in medium containing 1.05 mM Ca++ to effect attachment and growth to monolayer density. Medium is then switched to one containing 0.06 mM Ca++ to overcome “renewal inhibition” and to stimulate growth. In low Ca++ media, primary cultures become a long-term, continuous source of free-floating viable cells free of fibroblasts. A fundamental requirement for extended growth in primary culture is maintaining calcium levels at approximately 0.06 mM. Above 0.06 mM Ca++, cells divide only 3 to 4 times in primary cultures before terminal differentiation occurs. At 0.06 mM Ca++, cells continue to divide for periods of time determined partly by feeding schedule, but up to 6 mo. and 50 generations of (linear) growth. Cells released from monolayer were greater than 90% viable and yielded 105 cells/cm2 of attached cells every 72 h. Free-floating single cells readily replated and cloned, when transferred, without need of trypsin for dissociation. Long-term free-floating cells were typical mammary epithelium: (a) they formed domes and exhibited renewal inhibition, (b) they produced ductlike formations in collagen gels, (c) they contained epithelium-specific keratin filaments, and (d) they were diploid.
         datePublished:
         dateModified:
         pageStart:6
         pageEnd:12
         sameAs:https://doi.org/10.1007/BF02623435
         keywords:
            mammary
            calcium
            cell culture
            Plant Sciences
            Cell Biology
            Developmental Biology
            Plant Breeding/Biotechnology
            Plant Genetics and Genomics
         image:
         isPartOf:
            name:In Vitro Cellular & Developmental Biology
            issn:
               1475-2689
               0883-8364
            volumeNumber:22
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Herbert D. Soule
               affiliation:
                     name:Michigan Cancer Foundation
                     address:
                        name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Charles M. McGrath
               affiliation:
                     name:Michigan Cancer Foundation
                     address:
                        name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:A simplified method for passage and long-term growth of human mammary epithelial cells
      description:A method is described for culturing human mammary epithelial cells in primary culture and allowing more than 50 generations and a 1000-fold increase from starting inocula without need of enzymatic transfers. Organoids dissociated from breast tissue are plated in medium containing 1.05 mM Ca++ to effect attachment and growth to monolayer density. Medium is then switched to one containing 0.06 mM Ca++ to overcome “renewal inhibition” and to stimulate growth. In low Ca++ media, primary cultures become a long-term, continuous source of free-floating viable cells free of fibroblasts. A fundamental requirement for extended growth in primary culture is maintaining calcium levels at approximately 0.06 mM. Above 0.06 mM Ca++, cells divide only 3 to 4 times in primary cultures before terminal differentiation occurs. At 0.06 mM Ca++, cells continue to divide for periods of time determined partly by feeding schedule, but up to 6 mo. and 50 generations of (linear) growth. Cells released from monolayer were greater than 90% viable and yielded 105 cells/cm2 of attached cells every 72 h. Free-floating single cells readily replated and cloned, when transferred, without need of trypsin for dissociation. Long-term free-floating cells were typical mammary epithelium: (a) they formed domes and exhibited renewal inhibition, (b) they produced ductlike formations in collagen gels, (c) they contained epithelium-specific keratin filaments, and (d) they were diploid.
      datePublished:
      dateModified:
      pageStart:6
      pageEnd:12
      sameAs:https://doi.org/10.1007/BF02623435
      keywords:
         mammary
         calcium
         cell culture
         Plant Sciences
         Cell Biology
         Developmental Biology
         Plant Breeding/Biotechnology
         Plant Genetics and Genomics
      image:
      isPartOf:
         name:In Vitro Cellular & Developmental Biology
         issn:
            1475-2689
            0883-8364
         volumeNumber:22
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Herbert D. Soule
            affiliation:
                  name:Michigan Cancer Foundation
                  address:
                     name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Charles M. McGrath
            affiliation:
                  name:Michigan Cancer Foundation
                  address:
                     name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:In Vitro Cellular & Developmental Biology
      issn:
         1475-2689
         0883-8364
      volumeNumber:22
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Michigan Cancer Foundation
      address:
         name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
         type:PostalAddress
      name:Michigan Cancer Foundation
      address:
         name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Herbert D. Soule
      affiliation:
            name:Michigan Cancer Foundation
            address:
               name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
               type:PostalAddress
            type:Organization
      name:Charles M. McGrath
      affiliation:
            name:Michigan Cancer Foundation
            address:
               name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
      name:Department of Tumor Biology, Michigan Cancer Foundation, Detroit
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(103)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

Emails and Hosting {✉️}

Mail Servers:

mx.zoho.eu
mx2.zoho.eu
mx3.zoho.eu

Name Servers:

josh.ns.cloudflare.com
zita.ns.cloudflare.com

CDN Services {📦}

Crossref

4.71s.