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We began analyzing https://link.springer.com/article/10.1007/BF00420224, but it redirected us to https://link.springer.com/article/10.1007/BF00420224. The analysis below is for the second page.

Title[redir]:
Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe | Current Genetics
Description:
The major rRNA genes of the fission yeast Schizosaccharomyces pombe were mapped on chromosome III by plasmid integration. The integration vector YIp33 containing S. cerevisiae LEU2 gene was combined with the S. pombe rDNA. Since LEU2 complements S. pombe leu1 deficiency, it could be used as the genetic marker for integration. The 10.4 kb rDNA repeat contained ARS sequence, and therefore 2.4 kb and 0.7 kb subfragments not containing ARS were subcloned into YIp33 and transformed leu1 S. pombe cells to Leu+. Genetic analyses of the transformants indicated that the integrated rDNA resides in the long arm of the shortest chromosome III, tightly linked to ade5 (1.4 cM). This result is consistent with our previous finding that the DAPI-stained smallest chromosomes were associated with the nucleolus (Umesono et al. 1983).

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Keywords {🔍}

article, google, scholar, cas, pombe, integration, schizosaccharomyces, access, privacy, cookies, content, rrna, genes, toda, leu, genet, publish, search, genetics, yanagida, fission, rdna, pubmed, data, information, log, journal, research, mapping, february, nakaseko, niwa, yeast, chromosome, gene, ars, chromosomes, open, rna, discover, nurse, nature, cell, res, curr, sci, usa, download, springer, optional,

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month download article/chapter dapi-stained smallest chromosomes cerevisiae leu2 gene schizosaccharomyces cryophilus chromosomes schizosaccharomyces pombe published integration vector yip33 related subjects major rrna genes full article pdf privacy choices/manage cookies schizosaccharomyces pombe pombe leu1 deficiency integrated rdna resides check access dna segment instant access rrna genes european economic area 6-diamidino-2-phenylindole kb pombe rdna conditions privacy policy chromosome segregation defects article toda shortest chromosome iii accepting optional cookies petes td article log journal finder publish mapping pombe cells long arm article cite ars cell sci 52 privacy policy gene 8 personal data genetic analyses books a chromosome iii optional cookies manage preferences plasmid integration 7 kb subfragments subscription content similar content data protection essential cookies cookies skip leu2 complements

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         headline:Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe
         description:The major rRNA genes of the fission yeast Schizosaccharomyces pombe were mapped on chromosome III by plasmid integration. The integration vector YIp33 containing S. cerevisiae LEU2 gene was combined with the S. pombe rDNA. Since LEU2 complements S. pombe leu1 deficiency, it could be used as the genetic marker for integration. The 10.4 kb rDNA repeat contained ARS sequence, and therefore 2.4 kb and 0.7 kb subfragments not containing ARS were subcloned into YIp33 and transformed leu1 S. pombe cells to Leu+. Genetic analyses of the transformants indicated that the integrated rDNA resides in the long arm of the shortest chromosome III, tightly linked to ade5 (1.4 cM). This result is consistent with our previous finding that the DAPI-stained smallest chromosomes were associated with the nucleolus (Umesono et al. 1983).
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      headline:Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe
      description:The major rRNA genes of the fission yeast Schizosaccharomyces pombe were mapped on chromosome III by plasmid integration. The integration vector YIp33 containing S. cerevisiae LEU2 gene was combined with the S. pombe rDNA. Since LEU2 complements S. pombe leu1 deficiency, it could be used as the genetic marker for integration. The 10.4 kb rDNA repeat contained ARS sequence, and therefore 2.4 kb and 0.7 kb subfragments not containing ARS were subcloned into YIp33 and transformed leu1 S. pombe cells to Leu+. Genetic analyses of the transformants indicated that the integrated rDNA resides in the long arm of the shortest chromosome III, tightly linked to ade5 (1.4 cM). This result is consistent with our previous finding that the DAPI-stained smallest chromosomes were associated with the nucleolus (Umesono et al. 1983).
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         Nucleolar DNA
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         Microbial Genetics and Genomics
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