We are analyzing https://link.springer.com/article/10.1007/bf00331138.

Title:
Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation | Molecular Genetics and Genomics
Description:
A gene bank of partial Sau3A restriction fragments of S. pombe DNA has been constructed in the plasmid vector, pDB248′, which is capable of high frequency transformation of S. pombe. Procedures are described which enable plasmids to be recovered from S. pombe by their reintroduction into E. coli. These methods have been used to detect the S. pombe genes lys 1+, ade 6+ and his 2+ in the gene bank by complementation of mutant gene functions, and to physically isolate the lys 1+ gene.
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Keywords {🔍}

pombe, article, gene, yeast, schizosaccharomyces, google, scholar, nature, genes, nurse, privacy, cookies, content, beach, transformation, access, information, publish, search, bank, vector, data, log, journal, research, molecular, genetics, construction, bacterial, isolate, complementation, piper, plasmid, methods, isolation, fission, discover, cell, download, springer, function, optional, analysis, personal, parties, policy, find, track, general, mgg,

Topics {✒️}

mutant gene functions month download article/chapter pombe dna high frequency transformation high-frequency transformation mating-type genes related subjects replicating hybrid plasmid plasmid vector privacy choices/manage cookies schizosaccharomyces pombe full article pdf article beach gene bank lys 1+ gene european economic area isolate genes check access instant access conditions privacy policy fission yeast accepting optional cookies main content log journal finder publish function article molecular reproductive isolation cell size cell division auerbach rights article log privacy policy personal data article cite books a yeast centromere optional cookies manage preferences molecular rearrangement piper pombe isolation data protection essential cookies cookies skip subscription content similar content institution subscribe journal publish transformation

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         description:A gene bank of partial Sau3A restriction fragments of S. pombe DNA has been constructed in the plasmid vector, pDB248′, which is capable of high frequency transformation of S. pombe. Procedures are described which enable plasmids to be recovered from S. pombe by their reintroduction into E. coli. These methods have been used to detect the S. pombe genes lys 1+, ade 6+ and his 2+ in the gene bank by complementation of mutant gene functions, and to physically isolate the lys 1+ gene.
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            Mutant Gene
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            Plasmid Vector
            Frequency Transformation
            Plant Genetics and Genomics
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            Microbial Genetics and Genomics
            Animal Genetics and Genomics
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      description:A gene bank of partial Sau3A restriction fragments of S. pombe DNA has been constructed in the plasmid vector, pDB248′, which is capable of high frequency transformation of S. pombe. Procedures are described which enable plasmids to be recovered from S. pombe by their reintroduction into E. coli. These methods have been used to detect the S. pombe genes lys 1+, ade 6+ and his 2+ in the gene bank by complementation of mutant gene functions, and to physically isolate the lys 1+ gene.
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         Mutant Gene
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         Microbial Genetics and Genomics
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