
DOI . ORG {
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Title[redir]:
Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils | SpringerLink
Description:
Neutrophils are one of the first cells to respond to an inflammatory stimulus, and are equipped with an assortment of antimicrobial and proteolytic enzymes to disarm and degrade bacterial pathogens. A novel mechanism of bacterial trapping, termed neutrophil...
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Keywords {π}
neutrophil, extracellular, traps, neutrophils, article, pubmed, google, scholar, protocol, bone, cas, privacy, cookies, content, information, publish, mouse, search, immunity, nets, murine, marrowderived, glogauer, access, chapter, marrow, biol, download, toronto, springer, usd, personal, data, log, journal, research, models, innate, quantification, visualization, vong, sherman, book, brinkmann, goosmann, zychlinsky, cell, ebook, optional, media,
Topics {βοΈ}
month download article/chapter mouse bone marrow bone marrow neutrophils neutrophil extracellular traps protocol mouse models privacy choices/manage cookies device instant download functionally competent neutrophils european economic area immobilizing invading pathogens mouse models journal finder publish conditions privacy policy degrade bacterial pathogens accepting optional cookies visualize net structures main content log chapter log describe protocols protocol vong protocol usdΒ 49 neutrophil elastase social media permissions reprints innate immunity check access humana press ethics access protocol cite journal publish privacy policy allen rights books a marrow optional cookies information personal data manage preferences online nj data protection book series cell biol 191 cell biol 176 essential cookies cookies skip subscription content similar content bacterial trapping canada philip
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ScholarlyArticle:
headline:Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils
pageEnd:50
pageStart:41
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-481-4.jpg
genre:
Springer Protocols
isPartOf:
name:Mouse Models of Innate Immunity
isbn:
978-1-62703-481-4
978-1-62703-480-7
type:Book
publisher:
name:Humana Press
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Linda Vong
affiliation:
name:University of Toronto
address:
name:Faculty of Medicine, University of Toronto, Toronto, Canada
type:PostalAddress
type:Organization
type:Person
name:Philip M. Sherman
affiliation:
name:University of Toronto
address:
name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
type:PostalAddress
type:Organization
type:Person
name:Michael Glogauer
affiliation:
name:University of Toronto
address:
name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
type:PostalAddress
type:Organization
type:Person
keywords:Neutrophil extracellular trap, Bone marrow neutrophil, Nucleic acid stain, Histone H3, Elastase, Fluorescence microscopy
description:Neutrophils are one of the first cells to respond to an inflammatory stimulus, and are equipped with an assortment of antimicrobial and proteolytic enzymes to disarm and degrade bacterial pathogens. A novel mechanism of bacterial trapping, termed neutrophil extracellular traps (NETs), was recently described whereby neutrophils were shown to cast out web-like structures of chromatin, capturing and immobilizing invading pathogens. Herein we describe protocols to isolate murine bone marrow-derived neutrophils, and spectrophotometrically quantify, immunolabel, and visualize NET structures in vitro.
datePublished:2013
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