We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-481-4_5.

Title:
Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils | SpringerLink
Description:
Neutrophils are one of the first cells to respond to an inflammatory stimulus, and are equipped with an assortment of antimicrobial and proteolytic enzymes to disarm and degrade bacterial pathogens. A novel mechanism of bacterial trapping, termed neutrophil...
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Keywords {🔍}

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Schema {🗺️}

ScholarlyArticle:
      headline:Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils
      pageEnd:50
      pageStart:41
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-481-4.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Mouse Models of Innate Immunity
         isbn:
            978-1-62703-481-4
            978-1-62703-480-7
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Linda Vong
            affiliation:
                  name:University of Toronto
                  address:
                     name:Faculty of Medicine, University of Toronto, Toronto, Canada
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Philip M. Sherman
            affiliation:
                  name:University of Toronto
                  address:
                     name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Michael Glogauer
            affiliation:
                  name:University of Toronto
                  address:
                     name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Neutrophil extracellular trap, Bone marrow neutrophil, Nucleic acid stain, Histone H3, Elastase, Fluorescence microscopy
      description:Neutrophils are one of the first cells to respond to an inflammatory stimulus, and are equipped with an assortment of antimicrobial and proteolytic enzymes to disarm and degrade bacterial pathogens. A novel mechanism of bacterial trapping, termed neutrophil extracellular traps (NETs), was recently described whereby neutrophils were shown to cast out web-like structures of chromatin, capturing and immobilizing invading pathogens. Herein we describe protocols to isolate murine bone marrow-derived neutrophils, and spectrophotometrically quantify, immunolabel, and visualize NET structures in vitro.
      datePublished:2013
      isAccessibleForFree:
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Book:
      name:Mouse Models of Innate Immunity
      isbn:
         978-1-62703-481-4
         978-1-62703-480-7
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Toronto
      address:
         name:Faculty of Medicine, University of Toronto, Toronto, Canada
         type:PostalAddress
      name:University of Toronto
      address:
         name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
         type:PostalAddress
      name:University of Toronto
      address:
         name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
         type:PostalAddress
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      affiliation:
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            address:
               name:Faculty of Medicine, University of Toronto, Toronto, Canada
               type:PostalAddress
            type:Organization
      name:Philip M. Sherman
      affiliation:
            name:University of Toronto
            address:
               name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
               type:PostalAddress
            type:Organization
      name:Michael Glogauer
      affiliation:
            name:University of Toronto
            address:
               name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Faculty of Medicine, University of Toronto, Toronto, Canada
      name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
      name:Faculties of Medicine & Dentistry, University of Toronto, Toronto, Canada
WebPageElement:
      isAccessibleForFree:
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