DOI . ORG {
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Title[redir]:
Ultrastructural Analysis of Testicular Tissue and Sperm by Transmission and Scanning Electron Microscopy | SpringerLink
Description:
Transmission electron microscopy (TEM) studies have provided the basis for an in-depth understanding of the cell biology and normal functioning of the testis and male gametes and have opened the way to characterize the functional role played by specific organelles in...
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Keywords {🔍}
google, scholar, article, pubmed, cas, chemes, electron, ultrastructural, sperm, microscopy, cell, protocol, research, spermatogenesis, chapter, reprod, privacy, cookies, content, analysis, information, publish, biology, study, biol, author, function, search, molecular, normal, cells, men, university, download, utah, springer, usd, personal, data, log, journal, testicular, transmission, scanning, hector, book, methods, organelles, development, structural,
Topics {✒️}
month download article/chapter heat-mediated antigen retrieval privacy choices/manage cookies device instant download national research council scanning electron microscope scanning electron microscopy european economic area resin-embedded sections acid phosphatase localization head-neck attachment patho-physiological observations editor information editors salt lake city sperm tail protein stem cell spheroids journal finder publish conditions privacy policy organ surface features harvard medical school buenos aires children protocol spermatogenesis hector author information authors molecular genetic analysis accepting optional cookies sertoli cell differentiation severely asthenozoospermic men blood-testis barrier abnormal sperm phenotypes protocol chemes main content log functional role played fibrous sheath protocol usd 49 buenos aires university social media permissions reprints chicago press translational research ultrastructural analysis protocol cite electron microscopy chemes part present chapter antigen retrieval chapter log journal publish privacy policy check access ethics access
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ScholarlyArticle:
headline:Ultrastructural Analysis of Testicular Tissue and Sperm by Transmission and Scanning Electron Microscopy
pageEnd:348
pageStart:321
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-038-0.jpg
genre:
Springer Protocols
isPartOf:
name:Spermatogenesis
isbn:
978-1-62703-038-0
978-1-62703-037-3
type:Book
publisher:
name:Humana Press
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Hector E. Chemes
affiliation:
name:National Research Council (CONICET), Buenos Aires Children’s Hospital
address:
name:Center for Research in Endocrinology (CEDIE), National Research Council (CONICET), Buenos Aires Children’s Hospital, Buenos Aires, Argentina
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:Transmission electron microscopy, Ultrastructural immunocytochemistry, Ultrastructural histochemistry, Scanning electron microscopy, Testes, Semen, Spermatozoa, Spermatogenesis
description:Transmission electron microscopy (TEM) studies have provided the basis for an in-depth understanding of the cell biology and normal functioning of the testis and male gametes and have opened the way to characterize the functional role played by specific organelles in spermatogenesis and sperm function. The development of the scanning electron microscope (SEM) extended these boundaries to the recognition of cell and organ surface features and the architectural array of cells and tissues. The merging of immunocytochemical and histochemical approaches with electron microscopy has completed a series of technical improvements that integrate structural and functional features to provide a broad understanding of cell biology in health and disease. With these advances the detailed study of the intricate structural and molecular organization as well as the chemical composition of cellular organelles is now possible. Immunocytochemistry is used to identify proteins or other components and localize them in specific cells or organelles with high specificity and sensitivity, and histochemistry can be used to understand their function (i.e., enzyme activity). When these techniques are used in conjunction with electron microscopy their resolving power is further increased to subcellular levels. In the present chapter we will describe in detail various ultrastructural techniques that are now available for basic or translational research in reproductive biology and reproductive medicine. These include TEM, ultrastructural immunocytochemistry, ultrastructural histochemistry, and SEM.
datePublished:2013
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