We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-038-0_29.

Title:
Ultrastructural Analysis of Testicular Tissue and Sperm by Transmission and Scanning Electron Microscopy | SpringerLink
Description:
Transmission electron microscopy (TEM) studies have provided the basis for an in-depth understanding of the cell biology and normal functioning of the testis and male gametes and have opened the way to characterize the functional role played by specific organelles in...
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Schema {🗺️}

ScholarlyArticle:
      headline:Ultrastructural Analysis of Testicular Tissue and Sperm by Transmission and Scanning Electron Microscopy
      pageEnd:348
      pageStart:321
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-038-0.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Spermatogenesis
         isbn:
            978-1-62703-038-0
            978-1-62703-037-3
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Hector E. Chemes
            affiliation:
                  name:National Research Council (CONICET), Buenos Aires Children’s Hospital
                  address:
                     name:Center for Research in Endocrinology (CEDIE), National Research Council (CONICET), Buenos Aires Children’s Hospital, Buenos Aires, Argentina
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Transmission electron microscopy, Ultrastructural immunocytochemistry, Ultrastructural histochemistry, Scanning electron microscopy, Testes, Semen, Spermatozoa, Spermatogenesis
      description:Transmission electron microscopy (TEM) studies have provided the basis for an in-depth understanding of the cell biology and normal functioning of the testis and male gametes and have opened the way to characterize the functional role played by specific organelles in spermatogenesis and sperm function. The development of the scanning electron microscope (SEM) extended these boundaries to the recognition of cell and organ surface features and the architectural array of cells and tissues. The merging of immunocytochemical and histochemical approaches with electron microscopy has completed a series of technical improvements that integrate structural and functional features to provide a broad understanding of cell biology in health and disease. With these advances the detailed study of the intricate structural and molecular organization as well as the chemical composition of cellular organelles is now possible. Immunocytochemistry is used to identify proteins or other components and localize them in specific cells or organelles with high specificity and sensitivity, and histochemistry can be used to understand their function (i.e., enzyme activity). When these techniques are used in conjunction with electron microscopy their resolving power is further increased to subcellular levels. In the present chapter we will describe in detail various ultrastructural techniques that are now available for basic or translational research in reproductive biology and reproductive medicine. These include TEM, ultrastructural immunocytochemistry, ultrastructural histochemistry, and SEM.
      datePublished:2013
      isAccessibleForFree:
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Book:
      name:Spermatogenesis
      isbn:
         978-1-62703-038-0
         978-1-62703-037-3
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:National Research Council (CONICET), Buenos Aires Children’s Hospital
      address:
         name:Center for Research in Endocrinology (CEDIE), National Research Council (CONICET), Buenos Aires Children’s Hospital, Buenos Aires, Argentina
         type:PostalAddress
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Person:
      name:Hector E. Chemes
      affiliation:
            name:National Research Council (CONICET), Buenos Aires Children’s Hospital
            address:
               name:Center for Research in Endocrinology (CEDIE), National Research Council (CONICET), Buenos Aires Children’s Hospital, Buenos Aires, Argentina
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Center for Research in Endocrinology (CEDIE), National Research Council (CONICET), Buenos Aires Children’s Hospital, Buenos Aires, Argentina
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